Q22000 (PDE4_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable 3',5'-cyclic phosphodiesterase pde-4
EC=3.1.4.17

Gene names

Name:	pde-4
ORF Names:	R153.1

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	674 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.
Cofactor	Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Metal-binding cAMP
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cAMP catabolic process Inferred from sequence or structural similarity PubMed 16624912. Source: WormBase negative regulation of cAMP-mediated signaling Inferred from genetic interaction PubMed 16624912. Source: WormBase negative regulation of locomotion Inferred from mutant phenotype PubMed 16624912. Source: WormBase signal transduction Inferred from electronic annotation. Source: InterPro sleep Inferred from mutant phenotype PubMed 18185515. Source: WormBase
Cellular_component	synapse Inferred from direct assay PubMed 16624912. Source: WormBase
Molecular_function	3',5'-cyclic-AMP phosphodiesterase activity Inferred from sequence or structural similarity PubMed 16624912. Source: WormBase metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform d (identifier: Q22000-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform a (identifier: Q22000-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-125: Missing.
     126-150: SDDLREASSLRPVSRASSIASNEHG → MSISLINNNNRSVRRKFGESGFTLR

Note: No experimental confirmation available.

Isoform b (identifier: Q22000-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-62: Missing.
     63-91: VTIPGSNHKLTSSASSYHPPRELTVSTFS → MNGVARRKQFKLRPWQSTALPSRHDHYSC

Note: No experimental confirmation available.

Isoform c (identifier: Q22000-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-75: Missing.
     76-90: ASSYHPPRELTVSTF → MTSRRHTVWFVPGSR

Note: No experimental confirmation available.

Isoform e (identifier: Q22000-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-48: Missing.
     49-91: SRTPPAALPP...PRELTVSTFS → MCGSRRHLRR...LPSRHDHYSC

Note: No experimental confirmation available.

Isoform f (identifier: Q22000-6)

The sequence of this isoform differs from the canonical sequence as follows:
     151-158: HGDDLIVT → YVILYIFL
     159-674: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 674	674	Probable 3',5'-cyclic phosphodiesterase pde-4		PRO_0000198847
Sites
	Active site	407	1	Proton donor By similarity
	Metal binding	411	1	Divalent metal cation 1 By similarity
	Metal binding	447	1	Divalent metal cation 1 By similarity
	Metal binding	448	1	Divalent metal cation 1 By similarity
	Metal binding	448	1	Divalent metal cation 2 By similarity
	Metal binding	565	1	Divalent metal cation 1 By similarity
Natural variations
	Alternative sequence	1 – 125	125	Missing in isoform a.		VSP_014345
	Alternative sequence	1 – 75	75	Missing in isoform c.		VSP_014346
	Alternative sequence	1 – 62	62	Missing in isoform b.		VSP_014347
	Alternative sequence	1 – 48	48	Missing in isoform e.		VSP_014348
	Alternative sequence	49 – 91	43	SRTPP…VSTFS → MCGSRRHLRRNEDGMNGVAR RKQFKLRPWQSTALPSRHDH YSC in isoform e.		VSP_014349
	Alternative sequence	63 – 91	29	VTIPG…VSTFS → MNGVARRKQFKLRPWQSTAL PSRHDHYSC in isoform b.		VSP_014350
	Alternative sequence	76 – 90	15	ASSYH…TVSTF → MTSRRHTVWFVPGSR in isoform c.		VSP_014351
	Alternative sequence	126 – 150	25	SDDLR…SNEHG → MSISLINNNNRSVRRKFGES GFTLR in isoform a.		VSP_014352
	Alternative sequence	151 – 158	8	HGDDLIVT → YVILYIFL in isoform f.		VSP_014353
	Alternative sequence	159 – 674	516	Missing in isoform f.		VSP_014354

Sequences

	Sequence		Length	Mass (Da)
	Isoform d [UniParc]. Last modified July 5, 2005. Version 2. Checksum: 87BA310BE45DBD30	FASTA	674	75,389
	10 20 30 40 50 60 MPRRRGSSSS SSAAGGSGGG GGFGFSSLRR ELHLHNFFRT SSPSASSTSR TPPAALPPRT 70 80 90 100 110 120 SAVTIPGSNH KLTSSASSYH PPRELTVSTF SAGSATAADG LGGAHLTPSL SSSVHARRES 130 140 150 160 170 180 FLYRASDDLR EASSLRPVSR ASSIASNEHG HGDDLIVTPF AQLLASLRNV RSNLISITNI 190 200 210 220 230 240 QNSDDSRHAN RSAKRPPLHN IELPDDVVHC AHDTLEELDW CLDQLETIQT HRSVSEMASS 250 260 270 280 290 300 KFRKMLNKEL SHFAESSKSG TQVSKFLITT YMDKEEDEPS IEIEVPTEVQ GPSTSGPMTL 310 320 330 340 350 360 SILKKAQTAA MNKISGVRKL RAPSHDGHVP EYGVNCAREI AVHMQRLDDW GPDVFKIDEL 370 380 390 400 410 420 SKNHSLTVVT FSLLRQRNLF KTFEIHQSTL VTYLLNLEHH YRNNHYHNFI HAADVAQSMH 430 440 450 460 470 480 VLLMSPVLTE VFTDLEVLAA IFAGAVHDVD HPGFTNQYLI NSNNELAIMY NDESVLEQHH 490 500 510 520 530 540 LAVAFKLLQD SNCDFLANLS RKQRLQFRKI VIDMVLATDM SKHMSLLADL KTMVEAKKVA 550 560 570 580 590 600 GNNVIVLDKY NDKIQVLQSM IHLADLSNPT KPIELYQQWN QRIMEEYWRQ GDKEKELGLE 610 620 630 640 650 660 ISPMCDRGNV TIEKSQVGFI DYIVHPLYET WADLVYPDAQ NILDQLEENR EWYQSRIPEE 670 PDTARTVTED DEHK
	Isoform a [UniParc]. Checksum: DF2EE6F03925DE87	FASTA	549	62,904
	10 20 30 40 50 60 MSISLINNNN RSVRRKFGES GFTLRHGDDL IVTPFAQLLA SLRNVRSNLI SITNIQNSDD 70 80 90 100 110 120 SRHANRSAKR PPLHNIELPD DVVHCAHDTL EELDWCLDQL ETIQTHRSVS EMASSKFRKM 130 140 150 160 170 180 LNKELSHFAE SSKSGTQVSK FLITTYMDKE EDEPSIEIEV PTEVQGPSTS GPMTLSILKK 190 200 210 220 230 240 AQTAAMNKIS GVRKLRAPSH DGHVPEYGVN CAREIAVHMQ RLDDWGPDVF KIDELSKNHS 250 260 270 280 290 300 LTVVTFSLLR QRNLFKTFEI HQSTLVTYLL NLEHHYRNNH YHNFIHAADV AQSMHVLLMS 310 320 330 340 350 360 PVLTEVFTDL EVLAAIFAGA VHDVDHPGFT NQYLINSNNE LAIMYNDESV LEQHHLAVAF 370 380 390 400 410 420 KLLQDSNCDF LANLSRKQRL QFRKIVIDMV LATDMSKHMS LLADLKTMVE AKKVAGNNVI 430 440 450 460 470 480 VLDKYNDKIQ VLQSMIHLAD LSNPTKPIEL YQQWNQRIME EYWRQGDKEK ELGLEISPMC 490 500 510 520 530 540 DRGNVTIEKS QVGFIDYIVH PLYETWADLV YPDAQNILDQ LEENREWYQS RIPEEPDTAR TVTEDDEHK
	Isoform b [UniParc]. Checksum: 5E75F83B7D539255	FASTA	612	69,498
	10 20 30 40 50 60 MNGVARRKQF KLRPWQSTAL PSRHDHYSCA GSATAADGLG GAHLTPSLSS SVHARRESFL 70 80 90 100 110 120 YRASDDLREA SSLRPVSRAS SIASNEHGHG DDLIVTPFAQ LLASLRNVRS NLISITNIQN 130 140 150 160 170 180 SDDSRHANRS AKRPPLHNIE LPDDVVHCAH DTLEELDWCL DQLETIQTHR SVSEMASSKF 190 200 210 220 230 240 RKMLNKELSH FAESSKSGTQ VSKFLITTYM DKEEDEPSIE IEVPTEVQGP STSGPMTLSI 250 260 270 280 290 300 LKKAQTAAMN KISGVRKLRA PSHDGHVPEY GVNCAREIAV HMQRLDDWGP DVFKIDELSK 310 320 330 340 350 360 NHSLTVVTFS LLRQRNLFKT FEIHQSTLVT YLLNLEHHYR NNHYHNFIHA ADVAQSMHVL 370 380 390 400 410 420 LMSPVLTEVF TDLEVLAAIF AGAVHDVDHP GFTNQYLINS NNELAIMYND ESVLEQHHLA 430 440 450 460 470 480 VAFKLLQDSN CDFLANLSRK QRLQFRKIVI DMVLATDMSK HMSLLADLKT MVEAKKVAGN 490 500 510 520 530 540 NVIVLDKYND KIQVLQSMIH LADLSNPTKP IELYQQWNQR IMEEYWRQGD KEKELGLEIS 550 560 570 580 590 600 PMCDRGNVTI EKSQVGFIDY IVHPLYETWA DLVYPDAQNI LDQLEENREW YQSRIPEEPD 610 TARTVTEDDE HK
	Isoform c [UniParc]. Checksum: 30F3EC75C7A51CA6	FASTA	599	67,932
	10 20 30 40 50 60 MTSRRHTVWF VPGSRSAGSA TAADGLGGAH LTPSLSSSVH ARRESFLYRA SDDLREASSL 70 80 90 100 110 120 RPVSRASSIA SNEHGHGDDL IVTPFAQLLA SLRNVRSNLI SITNIQNSDD SRHANRSAKR 130 140 150 160 170 180 PPLHNIELPD DVVHCAHDTL EELDWCLDQL ETIQTHRSVS EMASSKFRKM LNKELSHFAE 190 200 210 220 230 240 SSKSGTQVSK FLITTYMDKE EDEPSIEIEV PTEVQGPSTS GPMTLSILKK AQTAAMNKIS 250 260 270 280 290 300 GVRKLRAPSH DGHVPEYGVN CAREIAVHMQ RLDDWGPDVF KIDELSKNHS LTVVTFSLLR 310 320 330 340 350 360 QRNLFKTFEI HQSTLVTYLL NLEHHYRNNH YHNFIHAADV AQSMHVLLMS PVLTEVFTDL 370 380 390 400 410 420 EVLAAIFAGA VHDVDHPGFT NQYLINSNNE LAIMYNDESV LEQHHLAVAF KLLQDSNCDF 430 440 450 460 470 480 LANLSRKQRL QFRKIVIDMV LATDMSKHMS LLADLKTMVE AKKVAGNNVI VLDKYNDKIQ 490 500 510 520 530 540 VLQSMIHLAD LSNPTKPIEL YQQWNQRIME EYWRQGDKEK ELGLEISPMC DRGNVTIEKS 550 560 570 580 590 QVGFIDYIVH PLYETWADLV YPDAQNILDQ LEENREWYQS RIPEEPDTAR TVTEDDEHK
	Isoform e [UniParc]. Checksum: 548A274EC8D470D6	FASTA	626	71,167
	10 20 30 40 50 60 MCGSRRHLRR NEDGMNGVAR RKQFKLRPWQ STALPSRHDH YSCAGSATAA DGLGGAHLTP 70 80 90 100 110 120 SLSSSVHARR ESFLYRASDD LREASSLRPV SRASSIASNE HGHGDDLIVT PFAQLLASLR 130 140 150 160 170 180 NVRSNLISIT NIQNSDDSRH ANRSAKRPPL HNIELPDDVV HCAHDTLEEL DWCLDQLETI 190 200 210 220 230 240 QTHRSVSEMA SSKFRKMLNK ELSHFAESSK SGTQVSKFLI TTYMDKEEDE PSIEIEVPTE 250 260 270 280 290 300 VQGPSTSGPM TLSILKKAQT AAMNKISGVR KLRAPSHDGH VPEYGVNCAR EIAVHMQRLD 310 320 330 340 350 360 DWGPDVFKID ELSKNHSLTV VTFSLLRQRN LFKTFEIHQS TLVTYLLNLE HHYRNNHYHN 370 380 390 400 410 420 FIHAADVAQS MHVLLMSPVL TEVFTDLEVL AAIFAGAVHD VDHPGFTNQY LINSNNELAI 430 440 450 460 470 480 MYNDESVLEQ HHLAVAFKLL QDSNCDFLAN LSRKQRLQFR KIVIDMVLAT DMSKHMSLLA 490 500 510 520 530 540 DLKTMVEAKK VAGNNVIVLD KYNDKIQVLQ SMIHLADLSN PTKPIELYQQ WNQRIMEEYW 550 560 570 580 590 600 RQGDKEKELG LEISPMCDRG NVTIEKSQVG FIDYIVHPLY ETWADLVYPD AQNILDQLEE 610 620 NREWYQSRIP EEPDTARTVT EDDEHK
	Isoform f [UniParc]. Checksum: 119F39EF1F6BEC58	FASTA	158	16,407
	10 20 30 40 50 60 MPRRRGSSSS SSAAGGSGGG GGFGFSSLRR ELHLHNFFRT SSPSASSTSR TPPAALPPRT 70 80 90 100 110 120 SAVTIPGSNH KLTSSASSYH PPRELTVSTF SAGSATAADG LGGAHLTPSL SSSVHARRES 130 140 150 FLYRASDDLR EASSLRPVSR ASSIASNEHG YVILYIFL

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO081170 Genomic DNA. Translation: CCD69643.1. FO081170 Genomic DNA. Translation: CCD69647.1. FO081170 Genomic DNA. Translation: CCD69649.1. FO081170 Genomic DNA. Translation: CCD69644.1. FO081170 Genomic DNA. Translation: CCD69648.1. FO081170 Genomic DNA. Translation: CCD69645.1.
PIR	T16769.
RefSeq	NP_495600.1. NM_063199.3. NP_495601.1. NM_063200.1. NP_871944.1. NM_182144.3. NP_871945.1. NM_182145.3. NP_871946.1. NM_182146.3. NP_871947.1. NM_182147.3.
UniGene	Cel.17824.
3D structure databases
ProteinModelPortal	Q22000.
SMR	Q22000. Positions 340-658.
ModBase	Search...
Protein-protein interaction databases
STRING	6239.R153.1d.
Proteomic databases
PaxDb	Q22000.
PRIDE	Q22000.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	R153.1d; R153.1d; R153.1.
GeneID	174235.
KEGG	cel:CELE_R153.1.
UCSC	R153.1b. c. elegans.
Organism-specific databases
CTD	174235.
WormBase	R153.1a; CE02038; WBGene00020114; pde-4. R153.1b; CE28641; WBGene00020114; pde-4. R153.1c; CE32060; WBGene00020114; pde-4. R153.1d; CE33438; WBGene00020114; pde-4. R153.1e; CE33439; WBGene00020114; pde-4. R153.1f; CE33440; WBGene00020114; pde-4.
Phylogenomic databases
eggNOG	NOG122287.
GeneTree	ENSGT00690000101692.
HOGENOM	HOG000236297.
InParanoid	Q22000.
KO	K01120.
Gene expression databases
ArrayExpress	Q22000.
Family and domain databases
Gene3D	1.10.1300.10. 1 hit.
InterPro	IPR003607. HD/PDEase_dom. IPR023088. PDEase. IPR002073. PDEase_catalytic_dom. IPR023174. PDEase_CS. [Graphical view]
Pfam	PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	883133.

Entry information

Entry name

PDE4_CAEEL

Accession

Primary (citable) accession number: Q22000
Secondary accession number(s): Q86NE7 , Q86NE8, Q86NE9, Q8IFZ3, Q95ZQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 5, 2005
Last modified:	April 3, 2013
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project