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Protein

Probable 3',5'-cyclic phosphodiesterase pde-4

Gene

pde-4

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei407 – 4071Proton donorBy similarity
Metal bindingi411 – 4111Divalent metal cation 1By similarity
Metal bindingi447 – 4471Divalent metal cation 1By similarity
Metal bindingi448 – 4481Divalent metal cation 1By similarity
Metal bindingi448 – 4481Divalent metal cation 2By similarity
Metal bindingi565 – 5651Divalent metal cation 1By similarity

GO - Molecular functioni

  • 3',5'-cyclic-AMP phosphodiesterase activity Source: WormBase
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cAMP catabolic process Source: WormBase
  • negative regulation of cAMP-mediated signaling Source: WormBase
  • negative regulation of locomotion Source: WormBase
  • signal transduction Source: InterPro
  • sleep Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cAMP, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_329728. DARPP-32 events.
REACT_354932. G alpha (s) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 3',5'-cyclic phosphodiesterase pde-4 (EC:3.1.4.17)
Gene namesi
Name:pde-4
ORF Names:R153.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiR153.1a; CE02038; WBGene00020114; pde-4.
R153.1b; CE28641; WBGene00020114; pde-4.
R153.1c; CE32060; WBGene00020114; pde-4.
R153.1d; CE33438; WBGene00020114; pde-4.
R153.1e; CE33439; WBGene00020114; pde-4.
R153.1f; CE33440; WBGene00020114; pde-4.

Subcellular locationi

GO - Cellular componenti

  • synapse Source: WormBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 674674Probable 3',5'-cyclic phosphodiesterase pde-4PRO_0000198847Add
BLAST

Proteomic databases

PaxDbiQ22000.
PRIDEiQ22000.

Expressioni

Gene expression databases

ExpressionAtlasiQ22000. baseline and differential.

Interactioni

Protein-protein interaction databases

STRINGi6239.R153.1d.

Structurei

3D structure databases

ProteinModelPortaliQ22000.
SMRiQ22000. Positions 340-658.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG122287.
GeneTreeiENSGT00760000118889.
HOGENOMiHOG000236297.
InParanoidiQ22000.
KOiK13293.
PhylomeDBiQ22000.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
InterProiIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform d (identifier: Q22000-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPRRRGSSSS SSAAGGSGGG GGFGFSSLRR ELHLHNFFRT SSPSASSTSR
60 70 80 90 100
TPPAALPPRT SAVTIPGSNH KLTSSASSYH PPRELTVSTF SAGSATAADG
110 120 130 140 150
LGGAHLTPSL SSSVHARRES FLYRASDDLR EASSLRPVSR ASSIASNEHG
160 170 180 190 200
HGDDLIVTPF AQLLASLRNV RSNLISITNI QNSDDSRHAN RSAKRPPLHN
210 220 230 240 250
IELPDDVVHC AHDTLEELDW CLDQLETIQT HRSVSEMASS KFRKMLNKEL
260 270 280 290 300
SHFAESSKSG TQVSKFLITT YMDKEEDEPS IEIEVPTEVQ GPSTSGPMTL
310 320 330 340 350
SILKKAQTAA MNKISGVRKL RAPSHDGHVP EYGVNCAREI AVHMQRLDDW
360 370 380 390 400
GPDVFKIDEL SKNHSLTVVT FSLLRQRNLF KTFEIHQSTL VTYLLNLEHH
410 420 430 440 450
YRNNHYHNFI HAADVAQSMH VLLMSPVLTE VFTDLEVLAA IFAGAVHDVD
460 470 480 490 500
HPGFTNQYLI NSNNELAIMY NDESVLEQHH LAVAFKLLQD SNCDFLANLS
510 520 530 540 550
RKQRLQFRKI VIDMVLATDM SKHMSLLADL KTMVEAKKVA GNNVIVLDKY
560 570 580 590 600
NDKIQVLQSM IHLADLSNPT KPIELYQQWN QRIMEEYWRQ GDKEKELGLE
610 620 630 640 650
ISPMCDRGNV TIEKSQVGFI DYIVHPLYET WADLVYPDAQ NILDQLEENR
660 670
EWYQSRIPEE PDTARTVTED DEHK

Note: No experimental confirmation available.

Length:674
Mass (Da):75,389
Last modified:July 5, 2005 - v2
Checksum:i87BA310BE45DBD30
GO
Isoform a (identifier: Q22000-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-125: Missing.
     126-150: SDDLREASSLRPVSRASSIASNEHG → MSISLINNNNRSVRRKFGESGFTLR

Note: No experimental confirmation available.

Show »
Length:549
Mass (Da):62,904
Checksum:iDF2EE6F03925DE87
GO
Isoform b (identifier: Q22000-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-62: Missing.
     63-91: VTIPGSNHKLTSSASSYHPPRELTVSTFS → MNGVARRKQFKLRPWQSTALPSRHDHYSC

Note: No experimental confirmation available.

Show »
Length:612
Mass (Da):69,498
Checksum:i5E75F83B7D539255
GO
Isoform c (identifier: Q22000-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-75: Missing.
     76-90: ASSYHPPRELTVSTF → MTSRRHTVWFVPGSR

Note: No experimental confirmation available.

Show »
Length:599
Mass (Da):67,932
Checksum:i30F3EC75C7A51CA6
GO
Isoform e (identifier: Q22000-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-48: Missing.
     49-91: SRTPPAALPP...PRELTVSTFS → MCGSRRHLRR...LPSRHDHYSC

Note: No experimental confirmation available.

Show »
Length:626
Mass (Da):71,167
Checksum:i548A274EC8D470D6
GO
Isoform f (identifier: Q22000-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     151-158: HGDDLIVT → YVILYIFL
     159-674: Missing.

Note: No experimental confirmation available.

Show »
Length:158
Mass (Da):16,407
Checksum:i119F39EF1F6BEC58
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 125125Missing in isoform a. CuratedVSP_014345Add
BLAST
Alternative sequencei1 – 7575Missing in isoform c. CuratedVSP_014346Add
BLAST
Alternative sequencei1 – 6262Missing in isoform b. CuratedVSP_014347Add
BLAST
Alternative sequencei1 – 4848Missing in isoform e. CuratedVSP_014348Add
BLAST
Alternative sequencei49 – 9143SRTPP…VSTFS → MCGSRRHLRRNEDGMNGVAR RKQFKLRPWQSTALPSRHDH YSC in isoform e. CuratedVSP_014349Add
BLAST
Alternative sequencei63 – 9129VTIPG…VSTFS → MNGVARRKQFKLRPWQSTAL PSRHDHYSC in isoform b. CuratedVSP_014350Add
BLAST
Alternative sequencei76 – 9015ASSYH…TVSTF → MTSRRHTVWFVPGSR in isoform c. CuratedVSP_014351Add
BLAST
Alternative sequencei126 – 15025SDDLR…SNEHG → MSISLINNNNRSVRRKFGES GFTLR in isoform a. CuratedVSP_014352Add
BLAST
Alternative sequencei151 – 1588HGDDLIVT → YVILYIFL in isoform f. CuratedVSP_014353
Alternative sequencei159 – 674516Missing in isoform f. CuratedVSP_014354Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081170 Genomic DNA. Translation: CCD69643.1.
FO081170 Genomic DNA. Translation: CCD69647.1.
FO081170 Genomic DNA. Translation: CCD69649.1.
FO081170 Genomic DNA. Translation: CCD69644.1.
FO081170 Genomic DNA. Translation: CCD69648.1.
FO081170 Genomic DNA. Translation: CCD69645.1.
PIRiT16769.
RefSeqiNP_495600.1. NM_063199.3. [Q22000-5]
NP_495601.1. NM_063200.1. [Q22000-2]
NP_871944.1. NM_182144.3. [Q22000-4]
NP_871945.1. NM_182145.3. [Q22000-1]
NP_871946.1. NM_182146.3. [Q22000-3]
NP_871947.1. NM_182147.3. [Q22000-6]
UniGeneiCel.17824.

Genome annotation databases

EnsemblMetazoaiR153.1d; R153.1d; WBGene00020114. [Q22000-1]
GeneIDi174235.
KEGGicel:CELE_R153.1.
UCSCiR153.1b. c. elegans. [Q22000-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081170 Genomic DNA. Translation: CCD69643.1.
FO081170 Genomic DNA. Translation: CCD69647.1.
FO081170 Genomic DNA. Translation: CCD69649.1.
FO081170 Genomic DNA. Translation: CCD69644.1.
FO081170 Genomic DNA. Translation: CCD69648.1.
FO081170 Genomic DNA. Translation: CCD69645.1.
PIRiT16769.
RefSeqiNP_495600.1. NM_063199.3. [Q22000-5]
NP_495601.1. NM_063200.1. [Q22000-2]
NP_871944.1. NM_182144.3. [Q22000-4]
NP_871945.1. NM_182145.3. [Q22000-1]
NP_871946.1. NM_182146.3. [Q22000-3]
NP_871947.1. NM_182147.3. [Q22000-6]
UniGeneiCel.17824.

3D structure databases

ProteinModelPortaliQ22000.
SMRiQ22000. Positions 340-658.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.R153.1d.

Proteomic databases

PaxDbiQ22000.
PRIDEiQ22000.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiR153.1d; R153.1d; WBGene00020114. [Q22000-1]
GeneIDi174235.
KEGGicel:CELE_R153.1.
UCSCiR153.1b. c. elegans. [Q22000-1]

Organism-specific databases

CTDi174235.
WormBaseiR153.1a; CE02038; WBGene00020114; pde-4.
R153.1b; CE28641; WBGene00020114; pde-4.
R153.1c; CE32060; WBGene00020114; pde-4.
R153.1d; CE33438; WBGene00020114; pde-4.
R153.1e; CE33439; WBGene00020114; pde-4.
R153.1f; CE33440; WBGene00020114; pde-4.

Phylogenomic databases

eggNOGiNOG122287.
GeneTreeiENSGT00760000118889.
HOGENOMiHOG000236297.
InParanoidiQ22000.
KOiK13293.
PhylomeDBiQ22000.

Enzyme and pathway databases

ReactomeiREACT_329728. DARPP-32 events.
REACT_354932. G alpha (s) signalling events.

Miscellaneous databases

NextBioi883133.
PROiQ22000.

Gene expression databases

ExpressionAtlasiQ22000. baseline and differential.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
InterProiIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.

Entry informationi

Entry nameiPDE4_CAEEL
AccessioniPrimary (citable) accession number: Q22000
Secondary accession number(s): Q86NE7
, Q86NE8, Q86NE9, Q8IFZ3, Q95ZQ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 5, 2005
Last modified: April 1, 2015
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.