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Q22000

- PDE4_CAEEL

UniProt

Q22000 - PDE4_CAEEL

Protein

Probable 3',5'-cyclic phosphodiesterase pde-4

Gene

pde-4

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 2 (05 Jul 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

    Cofactori

    Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei407 – 4071Proton donorBy similarity
    Metal bindingi411 – 4111Divalent metal cation 1By similarity
    Metal bindingi447 – 4471Divalent metal cation 1By similarity
    Metal bindingi448 – 4481Divalent metal cation 1By similarity
    Metal bindingi448 – 4481Divalent metal cation 2By similarity
    Metal bindingi565 – 5651Divalent metal cation 1By similarity

    GO - Molecular functioni

    1. 3',5'-cyclic-AMP phosphodiesterase activity Source: WormBase
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cAMP catabolic process Source: WormBase
    2. negative regulation of cAMP-mediated signaling Source: WormBase
    3. negative regulation of locomotion Source: WormBase
    4. signal transduction Source: InterPro
    5. sleep Source: WormBase

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    cAMP, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable 3',5'-cyclic phosphodiesterase pde-4 (EC:3.1.4.17)
    Gene namesi
    Name:pde-4
    ORF Names:R153.1
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome II

    Organism-specific databases

    WormBaseiR153.1a; CE02038; WBGene00020114; pde-4.
    R153.1b; CE28641; WBGene00020114; pde-4.
    R153.1c; CE32060; WBGene00020114; pde-4.
    R153.1d; CE33438; WBGene00020114; pde-4.
    R153.1e; CE33439; WBGene00020114; pde-4.
    R153.1f; CE33440; WBGene00020114; pde-4.

    Subcellular locationi

    GO - Cellular componenti

    1. synapse Source: WormBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 674674Probable 3',5'-cyclic phosphodiesterase pde-4PRO_0000198847Add
    BLAST

    Proteomic databases

    PaxDbiQ22000.
    PRIDEiQ22000.

    Interactioni

    Protein-protein interaction databases

    STRINGi6239.R153.1d.

    Structurei

    3D structure databases

    ProteinModelPortaliQ22000.
    SMRiQ22000. Positions 340-658.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG122287.
    GeneTreeiENSGT00660000095451.
    HOGENOMiHOG000236297.
    InParanoidiQ22000.
    KOiK01120.
    OMAiKASHINN.
    PhylomeDBiQ22000.

    Family and domain databases

    Gene3Di1.10.1300.10. 1 hit.
    InterProiIPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view]
    PfamiPF00233. PDEase_I. 1 hit.
    [Graphical view]
    PRINTSiPR00387. PDIESTERASE1.
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    PROSITEiPS00126. PDEASE_I. 1 hit.
    [Graphical view]

    Sequences (6)i

    Sequence statusi: Complete.

    This entry describes 6 isoformsi produced by alternative splicing. Align

    Isoform d (identifier: Q22000-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPRRRGSSSS SSAAGGSGGG GGFGFSSLRR ELHLHNFFRT SSPSASSTSR    50
    TPPAALPPRT SAVTIPGSNH KLTSSASSYH PPRELTVSTF SAGSATAADG 100
    LGGAHLTPSL SSSVHARRES FLYRASDDLR EASSLRPVSR ASSIASNEHG 150
    HGDDLIVTPF AQLLASLRNV RSNLISITNI QNSDDSRHAN RSAKRPPLHN 200
    IELPDDVVHC AHDTLEELDW CLDQLETIQT HRSVSEMASS KFRKMLNKEL 250
    SHFAESSKSG TQVSKFLITT YMDKEEDEPS IEIEVPTEVQ GPSTSGPMTL 300
    SILKKAQTAA MNKISGVRKL RAPSHDGHVP EYGVNCAREI AVHMQRLDDW 350
    GPDVFKIDEL SKNHSLTVVT FSLLRQRNLF KTFEIHQSTL VTYLLNLEHH 400
    YRNNHYHNFI HAADVAQSMH VLLMSPVLTE VFTDLEVLAA IFAGAVHDVD 450
    HPGFTNQYLI NSNNELAIMY NDESVLEQHH LAVAFKLLQD SNCDFLANLS 500
    RKQRLQFRKI VIDMVLATDM SKHMSLLADL KTMVEAKKVA GNNVIVLDKY 550
    NDKIQVLQSM IHLADLSNPT KPIELYQQWN QRIMEEYWRQ GDKEKELGLE 600
    ISPMCDRGNV TIEKSQVGFI DYIVHPLYET WADLVYPDAQ NILDQLEENR 650
    EWYQSRIPEE PDTARTVTED DEHK 674

    Note: No experimental confirmation available.

    Length:674
    Mass (Da):75,389
    Last modified:July 5, 2005 - v2
    Checksum:i87BA310BE45DBD30
    GO
    Isoform a (identifier: Q22000-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-125: Missing.
         126-150: SDDLREASSLRPVSRASSIASNEHG → MSISLINNNNRSVRRKFGESGFTLR

    Note: No experimental confirmation available.

    Show »
    Length:549
    Mass (Da):62,904
    Checksum:iDF2EE6F03925DE87
    GO
    Isoform b (identifier: Q22000-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-62: Missing.
         63-91: VTIPGSNHKLTSSASSYHPPRELTVSTFS → MNGVARRKQFKLRPWQSTALPSRHDHYSC

    Note: No experimental confirmation available.

    Show »
    Length:612
    Mass (Da):69,498
    Checksum:i5E75F83B7D539255
    GO
    Isoform c (identifier: Q22000-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-75: Missing.
         76-90: ASSYHPPRELTVSTF → MTSRRHTVWFVPGSR

    Note: No experimental confirmation available.

    Show »
    Length:599
    Mass (Da):67,932
    Checksum:i30F3EC75C7A51CA6
    GO
    Isoform e (identifier: Q22000-5) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-48: Missing.
         49-91: SRTPPAALPP...PRELTVSTFS → MCGSRRHLRR...LPSRHDHYSC

    Note: No experimental confirmation available.

    Show »
    Length:626
    Mass (Da):71,167
    Checksum:i548A274EC8D470D6
    GO
    Isoform f (identifier: Q22000-6) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         151-158: HGDDLIVT → YVILYIFL
         159-674: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:158
    Mass (Da):16,407
    Checksum:i119F39EF1F6BEC58
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 125125Missing in isoform a. CuratedVSP_014345Add
    BLAST
    Alternative sequencei1 – 7575Missing in isoform c. CuratedVSP_014346Add
    BLAST
    Alternative sequencei1 – 6262Missing in isoform b. CuratedVSP_014347Add
    BLAST
    Alternative sequencei1 – 4848Missing in isoform e. CuratedVSP_014348Add
    BLAST
    Alternative sequencei49 – 9143SRTPP…VSTFS → MCGSRRHLRRNEDGMNGVAR RKQFKLRPWQSTALPSRHDH YSC in isoform e. CuratedVSP_014349Add
    BLAST
    Alternative sequencei63 – 9129VTIPG…VSTFS → MNGVARRKQFKLRPWQSTAL PSRHDHYSC in isoform b. CuratedVSP_014350Add
    BLAST
    Alternative sequencei76 – 9015ASSYH…TVSTF → MTSRRHTVWFVPGSR in isoform c. CuratedVSP_014351Add
    BLAST
    Alternative sequencei126 – 15025SDDLR…SNEHG → MSISLINNNNRSVRRKFGES GFTLR in isoform a. CuratedVSP_014352Add
    BLAST
    Alternative sequencei151 – 1588HGDDLIVT → YVILYIFL in isoform f. CuratedVSP_014353
    Alternative sequencei159 – 674516Missing in isoform f. CuratedVSP_014354Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FO081170 Genomic DNA. Translation: CCD69643.1.
    FO081170 Genomic DNA. Translation: CCD69647.1.
    FO081170 Genomic DNA. Translation: CCD69649.1.
    FO081170 Genomic DNA. Translation: CCD69644.1.
    FO081170 Genomic DNA. Translation: CCD69648.1.
    FO081170 Genomic DNA. Translation: CCD69645.1.
    PIRiT16769.
    RefSeqiNP_495600.1. NM_063199.3. [Q22000-5]
    NP_495601.1. NM_063200.1. [Q22000-2]
    NP_871944.1. NM_182144.3. [Q22000-4]
    NP_871945.1. NM_182145.3. [Q22000-1]
    NP_871946.1. NM_182146.3. [Q22000-3]
    NP_871947.1. NM_182147.3. [Q22000-6]
    UniGeneiCel.17824.

    Genome annotation databases

    EnsemblMetazoaiR153.1d; R153.1d; WBGene00020114. [Q22000-1]
    GeneIDi174235.
    KEGGicel:CELE_R153.1.
    UCSCiR153.1b. c. elegans. [Q22000-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FO081170 Genomic DNA. Translation: CCD69643.1 .
    FO081170 Genomic DNA. Translation: CCD69647.1 .
    FO081170 Genomic DNA. Translation: CCD69649.1 .
    FO081170 Genomic DNA. Translation: CCD69644.1 .
    FO081170 Genomic DNA. Translation: CCD69648.1 .
    FO081170 Genomic DNA. Translation: CCD69645.1 .
    PIRi T16769.
    RefSeqi NP_495600.1. NM_063199.3. [Q22000-5 ]
    NP_495601.1. NM_063200.1. [Q22000-2 ]
    NP_871944.1. NM_182144.3. [Q22000-4 ]
    NP_871945.1. NM_182145.3. [Q22000-1 ]
    NP_871946.1. NM_182146.3. [Q22000-3 ]
    NP_871947.1. NM_182147.3. [Q22000-6 ]
    UniGenei Cel.17824.

    3D structure databases

    ProteinModelPortali Q22000.
    SMRi Q22000. Positions 340-658.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 6239.R153.1d.

    Proteomic databases

    PaxDbi Q22000.
    PRIDEi Q22000.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai R153.1d ; R153.1d ; WBGene00020114 . [Q22000-1 ]
    GeneIDi 174235.
    KEGGi cel:CELE_R153.1.
    UCSCi R153.1b. c. elegans. [Q22000-1 ]

    Organism-specific databases

    CTDi 174235.
    WormBasei R153.1a ; CE02038 ; WBGene00020114 ; pde-4.
    R153.1b ; CE28641 ; WBGene00020114 ; pde-4.
    R153.1c ; CE32060 ; WBGene00020114 ; pde-4.
    R153.1d ; CE33438 ; WBGene00020114 ; pde-4.
    R153.1e ; CE33439 ; WBGene00020114 ; pde-4.
    R153.1f ; CE33440 ; WBGene00020114 ; pde-4.

    Phylogenomic databases

    eggNOGi NOG122287.
    GeneTreei ENSGT00660000095451.
    HOGENOMi HOG000236297.
    InParanoidi Q22000.
    KOi K01120.
    OMAi KASHINN.
    PhylomeDBi Q22000.

    Miscellaneous databases

    NextBioi 883133.
    PROi Q22000.

    Family and domain databases

    Gene3Di 1.10.1300.10. 1 hit.
    InterProi IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view ]
    Pfami PF00233. PDEase_I. 1 hit.
    [Graphical view ]
    PRINTSi PR00387. PDIESTERASE1.
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    PROSITEi PS00126. PDEASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: Bristol N2.

    Entry informationi

    Entry nameiPDE4_CAEEL
    AccessioniPrimary (citable) accession number: Q22000
    Secondary accession number(s): Q86NE7
    , Q86NE8, Q86NE9, Q8IFZ3, Q95ZQ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: July 5, 2005
    Last modified: October 1, 2014
    This is version 88 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3