Skip Header

 
Contribute Send feedback
Read comments (1) or add your own

Reviewed, UniProtKB/Swiss-Prot Q21Y60 (HLDD_RHOFD)

Last modified June 16, 2009. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    ADP-L-glycero-D-manno-heptose-6-epimerase
    EC=5.1.3.20
Alternative name(s):
    ADP-L-glycero-beta-D-manno-heptose-6-epimerase
      Short name=ADP-glyceromanno-heptose 6-epimerase
      Short name=ADP-hep 6-epimerase
      Short name=AGME
Gene names
Name: hldD
Ordered Locus Names: Rfer_1561
OrganismRhodoferax ferrireducens (strain DSM 15236 / ATCC BAA-621 / T118) [Complete proteome] [HAMAP]
Taxonomic identifier338969 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Rhodoferax

Hide | Top

Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

Hide | Top

General annotation (Comments)

Function

Catalyzes the interconversion between ADP-D-glycero-beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose By similarity.

Catalytic activity

ADP-D-glycero-D-manno-heptose = ADP-L-glycero-D-manno-heptose. HAMAP MF_01601

Cofactor

Binds 1 NADP+ per subunit By similarity.

Pathway

Nucleotide-sugar biosynthesis; ADP-L-glycero-beta-D-manno-heptose biosynthesis; ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate: step 4/4. HAMAP MF_01601

Subunit structure

Homopentamer By similarity.

Domain

Contains a large N-terminal NADP-binding domain, and a smaller C-terminal substrate-binding domain By similarity.

Sequence similarities

Belongs to the sugar epimerase family. HldD subfamily.

Hide | Top

Ontologies

Keywords
   Biological processCarbohydrate metabolism
   LigandNADP
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: HAMAP

cellular metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular functionADP-glyceromanno-heptose 6-epimerase activity

Inferred from electronic annotation. Source: HAMAP

NADP or NADPH binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 340340ADP-L-glycero-D-manno-heptose-6-epimerase HAMAP MF_01601
PRO_0000255739

Regions

Nucleotide binding12 – 132NADP By similarity
Nucleotide binding33 – 342NADP By similarity
Nucleotide binding76 – 805NADP By similarity
Region209 – 2124Substrate binding By similarity

Sites

Active site1401Proton acceptor By similarity
Active site1861Proton acceptor By similarity
Binding site401NADP By similarity
Binding site931NADP By similarity
Binding site1441NADP By similarity
Binding site1771Substrate By similarity
Binding site1781NADP; via amide nitrogen By similarity
Binding site1861NADP By similarity
Binding site1881Substrate; via carbonyl oxygen By similarity
Binding site1951Substrate By similarity
Binding site2221Substrate By similarity
Binding site3011Substrate By similarity

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
Q21Y60-1 [UniParc].

Last modified April 18, 2006. Version 1.
Checksum: 83857F2D20C7B210

FASTA34037,384
        10         20         30         40         50         60 
MTRIVVTGAA GFIGSNIIQG LNARGLNDII AIDDLTQGDK FRNLAHLKIS DYVDASVFYD 

        70         80         90        100        110        120 
LFANGAYGQI EAVFHEGACS DTMESNGKYM MDNNYATSVN LFQACQKRGA RLLYASSAAT 

       130        140        150        160        170        180 
YGGSDTFRED PAFERPLNVY GYSKLLFDQR MRRECGNDFR RSVAGKTGQV VGFRYFNVYG 

       190        200        210        220        230        240 
PHEQHKGRMA SVAFHQFHQF QAEGKVKLFA DYGGYAAGAQ MRDFIFIDDV VAVNLWFFDH 

       250        260        270        280        290        300 
PGVSGIFNLG TGRAQPFNDV ASSVVNALRG LSGQTALGLE ALTEAGLIEY IPFPDALRGK 

       310        320        330        340 
YQSYTQADLS ALRATGCDHP FADVQTGVSN YVQWLAQQDS

« Hide

Hide | Top

References

[1]"Complete sequence of chromosome of Rhodoferax ferrireducens DSM 15236."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. expand/collapse author list , Kyrpides N., Ivanova N., Richardson P.
Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Hide | Top

Cross-references

Sequence databases

CP000267 Genomic DNA. Translation: ABD69293.1.
RefSeqYP_522824.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID3963268.
GenomeReviewsGene locus Rfer_1561 in contig CP000267_GR.
KEGGrfr:Rfer_1561.
NMPDRfig|338969.3.peg.3307.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ21Y60.
OMAQ21Y60. FGPNEYH.

Enzyme and pathway databases

BioCycRFER338969:RFER_1561-MON.

Family and domain databases

HAMAPMF_01601.
[Tree]
InterProIPR001509. Epimerase_deHydtase.
IPR011912. Heptose_epim.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHERPTHR10366:SF29. Heptose_epim. 1 hit.
PfamPF01370. Epimerase. 1 hit.
[Graphical view]
TIGRFAMsTIGR02197. heptose_epim. 1 hit.
ProtoNetSearch...

Hide | Top

Entry information

Entry nameHLDD_RHOFD
AccessionPrimary (citable) accession number: Q21Y60
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: April 18, 2006
Last modified: June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents