Reviewed, UniProtKB/Swiss-Prot Q21XH1 (MDH1_RHOFD)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase 1
    EC=1.1.1.37

Gene names

Name:	mdh1
Ordered Locus Names:	Rfer_1803

Organism

Rhodoferax ferrireducens (strain DSM 15236 / ATCC BAA-621 / T118) [Complete proteome] [HAMAP]

Taxonomic identifier

338969 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Rhodoferax

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity.
Catalytic activity	(S)-malate + NAD(+) = oxaloacetate + NADH.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 328	327	Malate dehydrogenase 1		PRO_0000294403
Regions
	Nucleotide binding	12 – 18	7	NAD By similarity
	Nucleotide binding	132 – 134	3	NAD By similarity
Sites
	Active site	190	1	Proton acceptor By similarity
	Binding site	95	1	Substrate By similarity
	Binding site	101	1	Substrate By similarity
	Binding site	108	1	NAD By similarity
	Binding site	115	1	NAD By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	165	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q21XH1-1 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: 2462BA46D0A8F272	FASTA	328	35,077
	10 20 30 40 50 60 MSKKPVRVAV TGAAGQIGYA ILFRIASGEM LGKDQPVILQ LLEVPVEKAQ QALQGVMMEL 70 80 90 100 110 120 QDCAFPLLAG MEAHSDPMTA FKDVDYALLI GSRPRGPGME RAELLAVNGA IFTAQGKALN 130 140 150 160 170 180 AVASRNVKVL VVGNPANTNA YIAMKSAPDL PAKNFTAMLR LDHNRALSQL ASKTGKAVAD 190 200 210 220 230 240 IEKMAVWGNH SPTMYADYRF ATINGESVKD MINDQDWNAN TFLPTVGKRG AAIIAARGVS 250 260 270 280 290 300 SAASAANAAI DHMRDWALGT NGKWVTMGIP SDGQYGIPKE TMFGFPVTCE GGEYKVVQNL 310 320 PIDAFSQECI NKTLKELQDE QAGVAHLL

References

[1]

"Complete sequence of chromosome of Rhodoferax ferrireducens DSM 15236." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Ivanova N., Richardson P. Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000267 Genomic DNA. Translation: ABD69532.1.
RefSeq	YP_523063.1.
3D structure databases
SMR	Q21XH1. Positions 3-328.
ModBase	Search...
Genome annotation databases
GeneID	3962200.
GenomeReviews	Gene locus Rfer_1803 in contig CP000267_GR.
KEGG	rfr:Rfer_1803.
NMPDR	fig|338969.3.peg.2020.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q21XH1.
Enzyme and pathway databases
BioCyc	RFER338969:RFER_1803-MON.
Family and domain databases
HAMAP	MF_01517. [Tree]
InterPro	IPR001557. L-lactate/malate_DHase. IPR001236. Lactate/malate_DHase. IPR015955. Lactate_DHase/Glyco_Ohase_4_C. IPR001252. Malate_DHase_AS. IPR010945. Malate_DHase_SF1. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23382. MDH_SF1. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
ProDom	PD003052. Mal_dehydrog. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01759. MalateDH-SF1. 1 hit.
PROSITE	PS00068. MDH. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH1_RHOFD

Accession

Primary (citable) accession number: Q21XH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	April 18, 2006
Last modified:	November 25, 2008
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)