Q21XF2 (Q21XF2_RHOFD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Glutamine--tRNA ligase HAMAP MF_00126
EC=6.1.1.18 HAMAP MF_00126

Alternative name(s):
Glutaminyl-tRNA synthetase HAMAP MF_00126

Gene names

Name:	glnS HAMAP MF_00126
Ordered Locus Names:	Rfer_1823

Organism

Rhodoferax ferrireducens (strain DSM 15236 / ATCC BAA-621 / T118) [Complete proteome] [HAMAP]

Taxonomic identifier

338969 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Albidiferax

Protein attributes

Sequence length	620 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP MF_00126 SAAS SAAS020059
Subunit structure	Monomer By similarity. HAMAP MF_00126 SAAS SAAS020059
Subcellular location	Cytoplasm By similarity HAMAP MF_00126.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. HAMAP MF_00126 RuleBase RU003489

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP MF_00126 RuleBase RU003489 SAAS SAAS004514
Cellular component	Cytoplasm HAMAP MF_00126 SAAS SAAS020059
Ligand	ATP-binding HAMAP MF_00126 SAAS SAAS004514 RuleBase RU003489 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP MF_00126 RuleBase RU003489 SAAS SAAS004514 EMBL ABD69551.1 Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Motif	87 – 97	11	"HIGH" region By similarity HAMAP MF_00126
	Motif	330 – 334	5	"KMSKS" region By similarity HAMAP MF_00126
Sites
	Binding site	333	1	ATP By similarity HAMAP MF_00126

Sequences

	Sequence		Length	Mass (Da)
	Q21XF2 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: DF7E8D69BD9DA927	FASTA	620	69,493
	10 20 30 40 50 60 MVLQSTAQSK IIRMSTPNPA TPPAHATKAA AAASEPVKPS NFLRQIIEHD LAAGTYATRR 70 80 90 100 110 120 WAGSPGDAAH HAQGQPDPAK IRTRFPPEPN GYLHIGHAKS ICLNFGLARD FGGVCHLRFD 130 140 150 160 170 180 DTNPEKEKLE YVTTIMNSVR WLGFEWNGSP TATPYQASDY FDFMYRAAEY LIEAGHAYVD 190 200 210 220 230 240 EQTVEQIRIN RGDFGKPGVD SPFRARTPAE NLARFREMRD GQHEDGAMVL RAKIDMASPN 250 260 270 280 290 300 INLRDPAIYR IRRATHHNTG DKWCIYPMYT FAHPIEDALE NITHSICTLE FEDQRPFYDW 310 320 330 340 350 360 LMERLIEGGL LSSPPPHQYE FARLNLTYVV TSKRKLAQLV YDHKVKGWDD PRMPTIVGLR 370 380 390 400 410 420 RRGYTPEAIQ LFTERIGVTK SDSWIDYSTL EGCLRETLDV CAPRAMAVLD PVKLVLTNWD 430 440 450 460 470 480 DVMGAGKLDD CSAPLHPHHP ELGKRAFKIG KEVWIDRTDF EETPPKGFFR LFPGNKVRLK 490 500 510 520 530 540 YGHVMECTGC TKDADGRVTE VQATLVPDTK SGTPGSDAVK VKGVITWVAV ADGLPVEVRL 550 560 570 580 590 600 YDRLFLEAQP DAGGKDFIEA LNPNSLKVVT AFVEPSLASA RADQKFQFER HGYFVADGVD 610 620 HTKAKPVFNL AVGLEDSWGR

References

[1]

"Complete sequence of chromosome of Rhodoferax ferrireducens DSM 15236." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Ivanova N., Richardson P. Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000267 Genomic DNA. Translation: ABD69551.1.
RefSeq	YP_523082.1. NC_007908.1.
3D structure databases
ProteinModelPortal	Q21XF2.
SMR	Q21XF2. Positions 40-617.
ModBase	Search...
Protein-protein interaction databases
STRING	Q21XF2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3961064.
GenomeReviews	Gene locus Rfer_1823 in contig CP000267_GR.
KEGG	rfr:Rfer_1823.
NMPDR	fig|338969.3.peg.2145.
PATRIC	23236267. VBIRhoFer131161_2144.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG334108.
OMA	RMPTIAG.
PhylomeDB	Q21XF2.
ProtClustDB	PRK05347.
Enzyme and pathway databases
BioCyc	RFER338969:RFER_1823-MONOMER.
Family and domain databases
HAMAP	MF_00126. Gln_tRNA_synth. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ib. IPR022861. Gln_tRNA_synth_bac. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 2 hits. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01886.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00440. GlnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q21XF2_RHOFD

Accession

Primary (citable) accession number: Q21XF2

Entry history

Integrated into UniProtKB/TrEMBL:	April 18, 2006
Last sequence update:	April 18, 2006
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)