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Q21X81 (TRMB_ALBFT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:Rfer_1896
OrganismAlbidiferax ferrireducens (strain ATCC BAA-621 / DSM 15236 / T118) (Rhodoferax ferrireducens) [Complete proteome] [HAMAP]
Taxonomic identifier338969 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeRhodoferax

Protein attributes

Sequence length245 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 245245tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288212

Regions

Region152 – 1576Interaction with RNA Potential
Region224 – 2274Substrate binding By similarity

Sites

Active site1461 By similarity
Binding site711S-adenosyl-L-methionine By similarity
Binding site961S-adenosyl-L-methionine By similarity
Binding site1231S-adenosyl-L-methionine By similarity
Binding site1461S-adenosyl-L-methionine By similarity
Binding site1501Substrate By similarity
Binding site1821Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q21X81 [UniParc].

Last modified April 18, 2006. Version 1.
Checksum: 26B59D4E9B94CCCB

FASTA24527,209
        10         20         30         40         50         60 
MPEGVAFPKE IKSFVRRAGR TTTGQAKALE DMGPQFLLPY QTSAIDFIAT YADSTGARGN 

        70         80         90        100        110        120 
NDINPGPVIL EIGFGMGEAT AHIAALMPEK NFLCCEVHEP GVGALLKRIG EQGLRNIRIV 

       130        140        150        160        170        180 
AHDAVEVIDH MLPLQSLDGV HIFFPDPWHK KKHNKRRLIQ SALIAKLAAR LKVGGYIHCA 

       190        200        210        220        230        240 
TDWQPYAEQI LEVLSKEPLL KNTATQTHPE LAGYAPKPYY RPLTKFENRG IKLGHGVWDI 


VFERV 

« Hide

References

[1]"Complete sequence of chromosome of Rhodoferax ferrireducens DSM 15236."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. expand/collapse author list , Kyrpides N., Ivanova N., Richardson P.
Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-621 / DSM 15236 / T118.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000267 Genomic DNA. Translation: ABD69622.1.
RefSeqYP_523153.1. NC_007908.1.

3D structure databases

ProteinModelPortalQ21X81.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING338969.Rfer_1896.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD69622; ABD69622; Rfer_1896.
GeneID3963225.
KEGGrfr:Rfer_1896.
PATRIC23236425. VBIRhoFer131161_2223.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMAQDNFLCC.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycAFER338969:GHU9-1915-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_ALBFT
AccessionPrimary (citable) accession number: Q21X81
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: April 18, 2006
Last modified: June 11, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways