Q21WY0 (LPXD_RHOFD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	Rfer_1998

Organism

Rhodoferax ferrireducens (strain DSM 15236 / ATCC BAA-621 / T118) [Complete proteome] [HAMAP]

Taxonomic identifier

338969 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Albidiferax

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_0000264423
Sites
	Active site	224	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q21WY0 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: F457CE153EFEBA35	FASTA	329	34,675
	10 20 30 40 50 60 MTLLLGSIVE LLGGDLQGDA SLKIEGLAPL ESATPQQLSF LSHPKYQNQL AASRAACVIV 70 80 90 100 110 120 SPEMRGVALA RGACIVTEQP YLYFARVTQL WKKSLPRTVR PQIHPSAVID PEAFVHPRAC 130 140 150 160 170 180 IGALCVIESG ASVGADTVLK SRVTVGENCV IGERCLLHSG VVIGADGFGF APHAGAWEKI 190 200 210 220 230 240 EQLGAVRIGN DVEIGANTCI DRGALQDTVI EDGVKLDNLI QIGHNVHVGK HTAMAGCAGV 250 260 270 280 290 300 AGSATIGAHC TLGGGAIVLG HLTLADGVNI SAATVVTRSL RKPGHYTGMF PIDDNAAWEK 310 320 NAASLKQLHS LRDRIRALED KLMTLRDRP

References

[1]

"Complete sequence of chromosome of Rhodoferax ferrireducens DSM 15236." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Ivanova N., Richardson P. Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 15236 / ATCC BAA-621 / T118.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000267 Genomic DNA. Translation: ABD69723.1.
RefSeq	YP_523254.1. NC_007908.1.
3D structure databases
ProteinModelPortal	Q21WY0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q21WY0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3962222.
GenomeReviews	Gene locus Rfer_1998 in contig CP000267_GR.
KEGG	rfr:Rfer_1998.
NMPDR	fig|338969.3.peg.1817.
PATRIC	23236641. VBIRhoFer131161_2330.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1044.
HOGENOM	HBG469615.
OMA	CFVGKNT.
PhylomeDB	Q21WY0.
ProtClustDB	PRK00892.
Enzyme and pathway databases
BioCyc	RFER338969:RFER_1998-MONOMER.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 4 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_RHOFD

Accession

Primary (citable) accession number: Q21WY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	April 18, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program