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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Rhodoferax ferrireducens (strain ATCC BAA-621 / DSM 15236 / T118) (Albidiferax ferrireducens)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei302 – 3021NucleophileUniRule annotation
Active sitei355 – 3551Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciAFER338969:GHU9-2183-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:Rfer_2162
OrganismiRhodoferax ferrireducens (strain ATCC BAA-621 / DSM 15236 / T118) (Albidiferax ferrireducens)
Taxonomic identifieri338969 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeRhodoferax
ProteomesiUP000008332 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6256251,4-alpha-glucan branching enzyme GlgBPRO_0000260688Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi338969.Rfer_2162.

Structurei

3D structure databases

ProteinModelPortaliQ21WG7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiDDADHNT.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q21WG7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSDHDIYLF REGTHAKLYN KLGCHLQSNG GANFAVWAPN AESVSVVGDW
60 70 80 90 100
NYWSGNVDRL DLRDDGSGIW QGFVENAVRG QGYKYRIQSS HGSYGVDKAD
110 120 130 140 150
PFAFYAEPPP ATASRVWSLE HDWKDDQWMS SRGPKNALDA PMSIYEIHLG
160 170 180 190 200
SWRRQDGHFL DYRELAHSLA DYVIEMGFTH VELMPVTEHP FYGSWGYQTT
210 220 230 240 250
GYFAPTSRFG TPQDFMHFVD HLHQRGIGVL LDWVPSHFPT DEHGLGYFDG
260 270 280 290 300
THLFEHSDPR QGFHPEWNSS IFNYGRNEVR SFLISSGLFW LDKYHLDGLR
310 320 330 340 350
VDGVASMLYL DYARKEDEWI PNRHGGRENL EAVDFLQTLN KAVYREYPDT
360 370 380 390 400
LTIAEESTAW PRVSRPTDMD GLGFGMKWNM GWMHDSLAYM QQEPVHRKYH
410 420 430 440 450
HHKLTFSLVY AFNENFVLPL SHDEVVHGKG SLLGKMPGDA WQQFANLRAL
460 470 480 490 500
FGYMWAHPGK KLLFMGGEFG QRREWTHDGE LEWWVTKLEG HAGLQRYVAQ
510 520 530 540 550
LNRVYRSLPA LYQLDFSPAG FEWVEADAAD TSVFAFLRKP REHGAPVLIV
560 570 580 590 600
SNMTPVPRTN YMLGVPLAGF WREVINSDAS EFGGSGWGNL GGVEASPVRF
610 620
HGRPHSVCLT LPPLSTLIFE HVPHA
Length:625
Mass (Da):71,407
Last modified:April 18, 2006 - v1
Checksum:iB90612F59B94E7AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000267 Genomic DNA. Translation: ABD69886.1.
RefSeqiWP_011464454.1. NC_007908.1.
YP_523417.1. NC_007908.1.

Genome annotation databases

EnsemblBacteriaiABD69886; ABD69886; Rfer_2162.
KEGGirfr:Rfer_2162.
PATRICi23236983. VBIRhoFer131161_2500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000267 Genomic DNA. Translation: ABD69886.1.
RefSeqiWP_011464454.1. NC_007908.1.
YP_523417.1. NC_007908.1.

3D structure databases

ProteinModelPortaliQ21WG7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi338969.Rfer_2162.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD69886; ABD69886; Rfer_2162.
KEGGirfr:Rfer_2162.
PATRICi23236983. VBIRhoFer131161_2500.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiDDADHNT.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciAFER338969:GHU9-2183-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome of Rhodoferax ferrireducens DSM 15236."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M.
    , Kyrpides N., Ivanova N., Richardson P.
    Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-621 / DSM 15236 / T118.

Entry informationi

Entry nameiGLGB_RHOFT
AccessioniPrimary (citable) accession number: Q21WG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: April 18, 2006
Last modified: April 1, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.