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Q21W43

- BIOB_ALBFT

UniProt

Q21W43 - BIOB_ALBFT

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Protein
Biotin synthase
Gene
bioB, Rfer_2292
Organism
Albidiferax ferrireducens (strain ATCC BAA-621 / DSM 15236 / T118) (Rhodoferax ferrireducens)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi75 – 751Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi79 – 791Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi82 – 821Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi119 – 1191Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi150 – 1501Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi210 – 2101Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi282 – 2821Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAFER338969:GHU9-2316-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Rfer_2292
OrganismiAlbidiferax ferrireducens (strain ATCC BAA-621 / DSM 15236 / T118) (Rhodoferax ferrireducens)
Taxonomic identifieri338969 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeRhodoferax
ProteomesiUP000008332: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 349349Biotin synthaseUniRule annotation
PRO_0000381579Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi338969.Rfer_2292.

Structurei

3D structure databases

ProteinModelPortaliQ21W43.
SMRiQ21W43. Positions 29-332.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiDETQALC.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q21W43-1 [UniParc]FASTAAdd to Basket

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MSPNPSQQSS AVAFHPSAAP ITLPESATWP LADVLALFEL PFNDLMFQAQ    50
QTHRAHFPKG DVELATLLSI KTGGCEEDCS YCPQAARYDT GVEAQKILEL 100
EQVLDAAREA RASGATRFCM GAAWRSPKER DLEKVEAMVR GVKQLGLETC 150
ATLGMLEEGQ ADRLKQAGLD YYNHNLDSAP EFYSNVISTR EYQDRLDTLG 200
RVRQAGLKIC CGGIVGMGES RQQRAGLIAQ LANLNPYPES VPVNHLVQVE 250
GTPLYGIEPL DPIEFVRTIA VARITMPKAR VRLSAGRRQM GDAVQAMCFL 300
AGANSIFYGD KLLTTGNPEA GDDLALLAKL GLKTHASTLT EAQKERCGG 349
Length:349
Mass (Da):37,881
Last modified:April 18, 2006 - v1
Checksum:i4A2A5FE99A60A7F4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000267 Genomic DNA. Translation: ABD70010.1.
RefSeqiYP_523541.1. NC_007908.1.

Genome annotation databases

EnsemblBacteriaiABD70010; ABD70010; Rfer_2292.
GeneIDi3963334.
KEGGirfr:Rfer_2292.
PATRICi23237257. VBIRhoFer131161_2634.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000267 Genomic DNA. Translation: ABD70010.1 .
RefSeqi YP_523541.1. NC_007908.1.

3D structure databases

ProteinModelPortali Q21W43.
SMRi Q21W43. Positions 29-332.
ModBasei Search...

Protein-protein interaction databases

STRINGi 338969.Rfer_2292.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABD70010 ; ABD70010 ; Rfer_2292 .
GeneIDi 3963334.
KEGGi rfr:Rfer_2292.
PATRICi 23237257. VBIRhoFer131161_2634.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi DETQALC.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci AFER338969:GHU9-2316-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome of Rhodoferax ferrireducens DSM 15236."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M.
    , Kyrpides N., Ivanova N., Richardson P.
    Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-621 / DSM 15236 / T118.

Entry informationi

Entry nameiBIOB_ALBFT
AccessioniPrimary (citable) accession number: Q21W43
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 18, 2006
Last modified: June 11, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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