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Q21W43 (BIOB_ALBFT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Rfer_2292
OrganismAlbidiferax ferrireducens (strain ATCC BAA-621 / DSM 15236 / T118) (Rhodoferax ferrireducens) [Complete proteome] [HAMAP]
Taxonomic identifier338969 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeRhodoferax

Protein attributes

Sequence length349 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 349349Biotin synthase HAMAP-Rule MF_01694
PRO_0000381579

Sites

Metal binding751Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding791Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding821Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1191Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1501Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2101Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2821Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q21W43 [UniParc].

Last modified April 18, 2006. Version 1.
Checksum: 4A2A5FE99A60A7F4

FASTA34937,881
        10         20         30         40         50         60 
MSPNPSQQSS AVAFHPSAAP ITLPESATWP LADVLALFEL PFNDLMFQAQ QTHRAHFPKG 

        70         80         90        100        110        120 
DVELATLLSI KTGGCEEDCS YCPQAARYDT GVEAQKILEL EQVLDAAREA RASGATRFCM 

       130        140        150        160        170        180 
GAAWRSPKER DLEKVEAMVR GVKQLGLETC ATLGMLEEGQ ADRLKQAGLD YYNHNLDSAP 

       190        200        210        220        230        240 
EFYSNVISTR EYQDRLDTLG RVRQAGLKIC CGGIVGMGES RQQRAGLIAQ LANLNPYPES 

       250        260        270        280        290        300 
VPVNHLVQVE GTPLYGIEPL DPIEFVRTIA VARITMPKAR VRLSAGRRQM GDAVQAMCFL 

       310        320        330        340 
AGANSIFYGD KLLTTGNPEA GDDLALLAKL GLKTHASTLT EAQKERCGG 

« Hide

References

[1]"Complete sequence of chromosome of Rhodoferax ferrireducens DSM 15236."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. expand/collapse author list , Kyrpides N., Ivanova N., Richardson P.
Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-621 / DSM 15236 / T118.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000267 Genomic DNA. Translation: ABD70010.1.
RefSeqYP_523541.1. NC_007908.1.

3D structure databases

ProteinModelPortalQ21W43.
SMRQ21W43. Positions 29-332.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING338969.Rfer_2292.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD70010; ABD70010; Rfer_2292.
GeneID3963334.
KEGGrfr:Rfer_2292.
PATRIC23237257. VBIRhoFer131161_2634.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMADETQALC.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycAFER338969:GHU9-2316-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_ALBFT
AccessionPrimary (citable) accession number: Q21W43
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 18, 2006
Last modified: June 11, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways