ID   MDH2_RHOFD              Reviewed;         334 AA.
AC   Q21VT3;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   18-APR-2006, sequence version 1.
DT   16-JUN-2009, entry version 22.
DE   RecName: Full=Malate dehydrogenase 2;
DE            EC=1.1.1.37;
GN   Name=mdh2; OrderedLocusNames=Rfer_2403;
OS   Rhodoferax ferrireducens (strain DSM 15236 / ATCC BAA-621 / T118).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Rhodoferax.
OX   NCBI_TaxID=338969;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T.,
RA   Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F.,
RA   Land M., Kyrpides N., Ivanova N., Richardson P.;
RT   "Complete sequence of chromosome of Rhodoferax ferrireducens DSM
RT   15236.";
RL   Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the reversible oxidation of malate to
CC       oxaloacetate (By similarity).
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = oxaloacetate + NADH.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 2 family.
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DR   EMBL; CP000267; ABD70120.1; -; Genomic_DNA.
DR   RefSeq; YP_523651.1; -.
DR   GeneID; 3962073; -.
DR   GenomeReviews; CP000267_GR; Rfer_2403.
DR   KEGG; rfr:Rfer_2403; -.
DR   NMPDR; fig|338969.3.peg.2508; -.
DR   HOGENOM; Q21VT3; -.
DR   OMA; Q21VT3; VIGRHAN.
DR   BioCyc; RFER338969:RFER_2403-MON; -.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0030060; F:L-malate dehydrogenase activity; IEA:HAMAP.
DR   GO; GO:0006096; P:glycolysis; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:HAMAP.
DR   HAMAP; MF_01517; -; 1.
DR   InterPro; IPR001557; L-lactate/malate_DH.
DR   InterPro; IPR001236; Lactate/malate_DH.
DR   InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR   InterPro; IPR001252; Malate_DH_AS.
DR   InterPro; IPR010945; Malate_DH_SF1.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.90.110.10; lact_mal_DH; 1.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR23382; MDH_SF1; 1.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR   ProDom; PD003052; Mal_dehydrog; 1.
DR   TIGRFAMs; TIGR01759; MalateDH-SF1; 1.
DR   PROSITE; PS00068; MDH; FALSE_NEG.
PE   3: Inferred from homology;
KW   Complete proteome; NAD; Oxidoreductase; Tricarboxylic acid cycle.
FT   CHAIN         1    334       Malate dehydrogenase 2.
FT                                /FTId=PRO_0000294404.
FT   NP_BIND      12     18       NAD (By similarity).
FT   NP_BIND     130    132       NAD (By similarity).
FT   ACT_SITE    191    191       Proton acceptor (By similarity).
FT   BINDING      93     93       Substrate (By similarity).
FT   BINDING      99     99       Substrate (By similarity).
FT   BINDING     106    106       NAD (By similarity).
FT   BINDING     113    113       NAD (By similarity).
FT   BINDING     132    132       Substrate (By similarity).
FT   BINDING     166    166       Substrate (By similarity).
SQ   SEQUENCE   334 AA;  35806 MW;  ED1746FB0D5A4D6C CRC64;
     MNQAPVRVTV TGAAGRVAYA LLFRIASGDM LGADQPVQLV LFDLPHAMKA MQGVVMELED
     CAFPLLTEII ATDDPVLAFS DTQIALLVSA RPRAVGAERL EVLADNAKIF AAQGAVIGRH
     ANPDCKVLVV GNPCNTNACV AMKAAQKFSR IPARNFAALL RLDHNRALAQ LALKTGRPVG
     GVKRLAVWGN HSPTVYADDR FTTIDGDSVP AIIDNIAWHH DTLVRTVDKR GEAILAARGL
     YAEASAASAA IDQMRDWWLG TRGEWTTMGV VSDGAYGVPA GLVFGFPVIT DCRDYRIVPG
     LAVDAFARGM IDVNVRELTA ELEIVKPLLP ELFG
//