Reviewed, UniProtKB/Swiss-Prot Q21VT3 (MDH2_RHOFD)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase 2
    EC=1.1.1.37

Gene names

Name:	mdh2
Ordered Locus Names:	Rfer_2403

Organism

Rhodoferax ferrireducens (strain DSM 15236 / ATCC BAA-621 / T118) [Complete proteome] [HAMAP]

Taxonomic identifier

338969 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Rhodoferax

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP MF_01517
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP MF_01517
Subunit structure	Homodimer By similarity. HAMAP MF_01517
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Malate dehydrogenase 2 HAMAP MF_01517		PRO_0000294404
Regions
	Nucleotide binding	12 – 18	7	NAD By similarity
	Nucleotide binding	130 – 132	3	NAD By similarity
Sites
	Active site	191	1	Proton acceptor By similarity
	Binding site	93	1	Substrate By similarity
	Binding site	99	1	Substrate By similarity
	Binding site	106	1	NAD By similarity
	Binding site	113	1	NAD By similarity
	Binding site	132	1	Substrate By similarity
	Binding site	166	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q21VT3-1 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: ED1746FB0D5A4D6C	FASTA	334	35,806
	10 20 30 40 50 60 MNQAPVRVTV TGAAGRVAYA LLFRIASGDM LGADQPVQLV LFDLPHAMKA MQGVVMELED 70 80 90 100 110 120 CAFPLLTEII ATDDPVLAFS DTQIALLVSA RPRAVGAERL EVLADNAKIF AAQGAVIGRH 130 140 150 160 170 180 ANPDCKVLVV GNPCNTNACV AMKAAQKFSR IPARNFAALL RLDHNRALAQ LALKTGRPVG 190 200 210 220 230 240 GVKRLAVWGN HSPTVYADDR FTTIDGDSVP AIIDNIAWHH DTLVRTVDKR GEAILAARGL 250 260 270 280 290 300 YAEASAASAA IDQMRDWWLG TRGEWTTMGV VSDGAYGVPA GLVFGFPVIT DCRDYRIVPG 310 320 330 LAVDAFARGM IDVNVRELTA ELEIVKPLLP ELFG

References

[1]

"Complete sequence of chromosome of Rhodoferax ferrireducens DSM 15236." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Ivanova N., Richardson P. Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000267 Genomic DNA. Translation: ABD70120.1.
RefSeq	YP_523651.1.
3D structure databases
SMR	Q21VT3. Positions 4-329.
ModBase	Search...
Protein-protein interaction databases
STRING	Q21VT3.
Genome annotation databases
GeneID	3962073.
GenomeReviews	Gene locus Rfer_2403 in contig CP000267_GR.
KEGG	rfr:Rfer_2403.
NMPDR	fig|338969.3.peg.2508.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HBG289884.
Enzyme and pathway databases
BioCyc	RFER338969:RFER_2403-MONOMER.
Family and domain databases
HAMAP	MF_01517. Malate_dehydrog_2. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR010945. Malate_DH_SF1. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23382. MDH_SF1. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
TIGRFAMs	TIGR01759. MalateDH-SF1. 1 hit.
PROSITE	PS00068. MDH. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH2_RHOFD

Accession

Primary (citable) accession number: Q21VT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	April 18, 2006
Last modified:	February 9, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)