Reviewed, UniProtKB/Swiss-Prot Q21UY4 (F16A2_RHOFD)

Names and origin

Protein names

Recommended name:
    Fructose-1,6-bisphosphatase class 1 2
      Short name=FBPase class 1 2
    EC=3.1.3.11
Alternative name(s):
    D-fructose-1,6-bisphosphate 1-phosphohydrolase class 1 2

Gene names

Name:	fbp2
Ordered Locus Names:	Rfer_2704

Organism

Rhodoferax ferrireducens (strain DSM 15236 / ATCC BAA-621 / T118) [Complete proteome] [HAMAP]

Taxonomic identifier

338969 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Rhodoferax

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate. HAMAP MF_01855
Cofactor	Binds 2 magnesium ions per subunit By similarity.
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_01855
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the FBPase class 1 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fructose 1,6-bisphosphate 1-phosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Fructose-1,6-bisphosphatase class 1 2 HAMAP MF_01855		PRO_0000364673
Regions
	Region	117 – 120	4	Substrate binding By similarity
Sites
	Metal binding	92	1	Magnesium 1 By similarity
	Metal binding	114	1	Magnesium 1 By similarity
	Metal binding	114	1	Magnesium 2 By similarity
	Metal binding	116	1	Magnesium 1; via carbonyl oxygen By similarity
	Metal binding	117	1	Magnesium 2 By similarity
	Metal binding	280	1	Magnesium 2 By similarity
	Binding site	208	1	Substrate By similarity
	Binding site	274	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q21UY4-1 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: 6566DD3D54740C61	FASTA	334	37,202
	10 20 30 40 50 60 MNKRISLTRY LVEQQRMEGH IPAQLRLLLE VVARACKRIS LTVNKGALGG VLGSAETENI 70 80 90 100 110 120 QGEMQKKLDI IANEVLIEAN EWGGHLAAMA SEEMDGIYIV PNRYPQGEYL LMFDPLDGSS 130 140 150 160 170 180 NIDVNVSIGT IFSVLVKPDG QGVLEQDFLQ PGNQQVAAGY CVFGPQTTLV LTVGDGVAMF 190 200 210 220 230 240 TLDREQGSFI LTAENMRVPV DTQEFAVNMS NQRHWDTPVK RYIDECLQGK DGPRGKDFNM 250 260 270 280 290 300 RWVGSMVADV HRILTRGGVF LYPWDKREPE KPGKLRLMYE ANPMAWLIEQ AGGAASTGKQ 310 320 330 RILDLQPGKL HERVSVFLGS KNEVERIARY HGEA

References

[1]

"Complete sequence of chromosome of Rhodoferax ferrireducens DSM 15236." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Ivanova N., Richardson P. Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000267 Genomic DNA. Translation: ABD70419.1.
RefSeq	YP_523950.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q21UY4.
Genome annotation databases
GeneID	3964250.
GenomeReviews	Gene locus Rfer_2704 in contig CP000267_GR.
KEGG	rfr:Rfer_2704.
NMPDR	fig|338969.3.peg.3187.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q21UY4.
OMA	QRHWEAP.
Enzyme and pathway databases
BioCyc	RFER338969:RFER_2704-MON.
Family and domain databases
HAMAP	MF_01855. [Tree]
InterPro	IPR000146. Fructose_bisphosphatase. IPR020548. Fructose_bisphosphatase_AS. [Graphical view]
PANTHER	PTHR11556. In_FB_phphtase. 1 hit.
Pfam	PF00316. FBPase. 1 hit. [Graphical view]
PRINTS	PR00115. F16BPHPHTASE.
ProDom	PD001491. In_FB_phphtase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00124. FBPASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F16A2_RHOFD

Accession

Primary (citable) accession number: Q21UY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	April 18, 2006
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)