Reviewed, UniProtKB/Swiss-Prot Q21U05 (GUAC_RHOFD)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	Rfer_3037

Organism

Rhodoferax ferrireducens (strain DSM 15236 / ATCC BAA-621 / T118) [Complete proteome] [HAMAP]

Taxonomic identifier

338969 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Rhodoferax

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity.
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_01511
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	GMP reductase HAMAP MF_01511		PRO_0000294283
Regions
	Nucleotide binding	202 – 225	24	NADP Potential
Sites
	Active site	173	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q21U05-1 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: 0DBB4DB4564FB50D	FASTA	325	35,813
	10 20 30 40 50 60 MEIFDYDNIL LLPRKCRVES RSECDASVEL GRQRFRIPVV PANMKTVVDE SICVWLAQNS 70 80 90 100 110 120 YFYVMHRFDL DNVQFVKNMK ARGLYASISL GVKKPDYDTV DQLLALGLVP DYITIDIAHG 130 140 150 160 170 180 HADSVKNMIG YLKEKMPTVF IIAGNVATPE AVIDLENWGA DATKVGIGPG KVCITKLKTG 190 200 210 220 230 240 FGTGGWQLSA VKWCARVATK PIIADGGIHE HGDIAKSIRF GATMVMIGSM LAGHEESPGK 250 260 270 280 290 300 SVEVDGKLYK EYYGSASDFN KGEYKHVEGK RILEPVKGKL ADTLIEMEQD VQSSISYSGG 310 320 KRLMDIRKVN YVTLGGDNAG EHLLM

References

[1]

"Complete sequence of chromosome of Rhodoferax ferrireducens DSM 15236." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Ivanova N., Richardson P. Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000267 Genomic DNA. Translation: ABD70748.1.
RefSeq	YP_524279.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3961012.
GenomeReviews	Gene locus Rfer_3037 in contig CP000267_GR.
KEGG	rfr:Rfer_3037.
NMPDR	fig|338969.3.peg.3457.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q21U05.
OMA	Q21U05. NSRSECD.
Enzyme and pathway databases
BioCyc	RFER338969:RFER_3037-MON.
Family and domain databases
HAMAP	MF_01511. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_RHOFD

Accession

Primary (citable) accession number: Q21U05

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	April 18, 2006
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)