Reviewed, UniProtKB/Swiss-Prot Q21RR4 (ACEK_RHOFD)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	Rfer_3840

Organism

Rhodoferax ferrireducens (strain DSM 15236 / ATCC BAA-621 / T118) [Complete proteome] [HAMAP]

Taxonomic identifier

338969 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Rhodoferax

Protein attributes

Sequence length	615 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity.
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 615	615	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000288297
Regions
	Nucleotide binding	325 – 331	7	ATP By similarity
Sites
	Active site	381	1	By similarity
	Binding site	346	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q21RR4-1 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: 716ED446F0A86DEC	FASTA	615	71,666
	10 20 30 40 50 60 MFPTRLDSSL AYDIAKAMMD GFNRHYRLFR TESARAKHRF ETADWHGQQR AQRDRIEFYD 70 80 90 100 110 120 LRVRECFMRL AREFKADEQP MEVWQQVKLH YIGLLVDHHQ PELAETFFNS VTTKILHHSY 130 140 150 160 170 180 FQNDFIFVRP AVSTEYIEND ESETSPTYRS YYPTHDSLAE VLVQMVLDFD LRCPFADLDS 190 200 210 220 230 240 DAARVQAAMV EQLGEVKLRA NFQLQVLSGL FFRNKGCYIV GKLINGFNEF PFALPVLYGR 250 260 270 280 290 300 AGQLVIDAVL FGEDDLLMLF SFARAYFMVD MEIPSAYVQY LRSMMPRKPR NEIYNALGLA 310 320 330 340 350 360 KQGKTLFYRD FLHHQRHSTD KFRIAPGIKG MVMLVFDLPS FPYVFKVIKD FYPPPKDTTR 370 380 390 400 410 420 EQIKAKYLLV KQHDRVGRMA DTLEYSEVGF PRARFDDELI AELEKFAPSQ LEISDRDGDG 430 440 450 460 470 480 RIEVIIKHVY IERRMIPLNI YLQEAFDAGG ANPDDTSPAA LRAREQIERG VIEYGNALKD 490 500 510 520 530 540 LVAANIFPGD MLWKNFGITR HGKVVFYDYD EIEYITDCNF RKVPQARNEE DEMSGEVWYR 550 560 570 580 590 600 VGPKDVFPET FAPFLLGNPF VREVFMKHHA ELLDAAFWQS HKERIQAGHV YDVFPYDQGK 610 RFHPEISDAQ VPSSI

References

[1]

"Complete sequence of chromosome of Rhodoferax ferrireducens DSM 15236." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Ivanova N., Richardson P. Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000267 Genomic DNA. Translation: ABD71539.1.
RefSeq	YP_525070.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q21RR4.
Genome annotation databases
GeneID	3961442.
GenomeReviews	Gene locus Rfer_3840 in contig CP000267_GR.
KEGG	rfr:Rfer_3840.
NMPDR	fig|338969.3.peg.4025.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q21RR4.
OMA	FYRDFLH.
Enzyme and pathway databases
BioCyc	RFER338969:RFER_3840-MON.
Family and domain databases
HAMAP	MF_00747. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACEK_RHOFD

Accession

Primary (citable) accession number: Q21RR4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	April 18, 2006
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)