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Q21PN1

- NAPA_SACD2

UniProt

Q21PN1 - NAPA_SACD2

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (18 Apr 2006)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi48 – 481Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi55 – 551Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi83 – 831Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciSDEG203122:GI2M-84-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:Sde_0084
    OrganismiSaccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)
    Taxonomic identifieri203122 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeSaccharophagus
    ProteomesiUP000001947: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Tat-type signalUniRule annotationAdd
    BLAST
    Chaini30 – 831802Periplasmic nitrate reductasePRO_0000256076Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi203122.Sde_0084.

    Structurei

    3D structure databases

    ProteinModelPortaliQ21PN1.
    SMRiQ21PN1. Positions 43-829.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini41 – 97574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiLVYMHPE.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q21PN1-1 [UniParc]FASTAAdd to Basket

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    MTVTRRDFVR HQALATAAAA AGVAVPAAAT NIVTTAADNK LVWSKAPCRF    50
    CGTGCSVNVA TKEGRVVATH GDIKSPVNRG LNCVKGYFLS KVMYGEDRLT 100
    QPLLRKKNGV YAKDGEFEPV TWEEAFTIMA EKFKKTLKEK GPEGIGMFGS 150
    GQWTVWEGYA ASKLMKAGFR SNNIDPNARH CMASAVGGFM RTFGIDEPMG 200
    CYDDFEHADA FVLWGSNMAE MHPILWTRIA DRRLSYPHVQ VAVMSTYEHR 250
    SFDLADLGVV FTPQSDLAIA NFIANYIIQN KKVNWDFVKK HTNFRVGTTD 300
    IGYGLRPEHP LQKAAKNADS AGASEPIDFE TYAKFVADYT VEKASEISGV 350
    SEDKLIKLAK LYADPNIKVM SLWTMGVNQH TRGVWMNNLI YNIHLLTGKI 400
    SEPGNSPFSL TGQPSACGTA REVGTFSHRL PADMVVTNPK HRAYAEKIWK 450
    LPEGSIPEKV GAHAVLQSRK LKDGEINAYW VQVNNNVQAG PNINEEVLPG 500
    YRNPQNFIVV SDAYPTVTAQ AADLILPSAM WVEKEGAYGN AERRTQFWHQ 550
    LVNAPGDARS DLWQLVEFSK YFKVEEVWPA DLIAKMPEAK GKTLFDILYK 600
    NGKVNKFPLA QVSKDYENKE ADAFGFYIQK GLFEEYAEFG RGHGHDLADF 650
    DRYHEERGLR WPVVNGEETL WRYREGSDPY VEKGKGVQFY GKPDGKAIIF 700
    ALPYEPPAES PNKEYPFWLC TGRVIEHWHS GSMTQRVPEL YKAFPDAVCF 750
    MHPEDARSAG LRRGDKVKLQ SIRGHIITRI ETRGRNKPPK GLVFVPWFDA 800
    RQLINKVTLD ATDPISKQTD FKKCAVKVER V 831
    Length:831
    Mass (Da):93,164
    Last modified:April 18, 2006 - v1
    Checksum:i8B6EA1C70B7AF770
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000282 Genomic DNA. Translation: ABD79348.1.
    RefSeqiYP_525560.1. NC_007912.1.

    Genome annotation databases

    EnsemblBacteriaiABD79348; ABD79348; Sde_0084.
    GeneIDi3967323.
    KEGGisde:Sde_0084.
    PATRICi23398763. VBISacDeg56404_0090.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000282 Genomic DNA. Translation: ABD79348.1 .
    RefSeqi YP_525560.1. NC_007912.1.

    3D structure databases

    ProteinModelPortali Q21PN1.
    SMRi Q21PN1. Positions 43-829.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 203122.Sde_0084.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABD79348 ; ABD79348 ; Sde_0084 .
    GeneIDi 3967323.
    KEGGi sde:Sde_0084.
    PATRICi 23398763. VBISacDeg56404_0090.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi LVYMHPE.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci SDEG203122:GI2M-84-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 2-40 / ATCC 43961 / DSM 17024.

    Entry informationi

    Entry nameiNAPA_SACD2
    AccessioniPrimary (citable) accession number: Q21PN1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2006
    Last sequence update: April 18, 2006
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3