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Q21PN1 (NAPA_SACD2) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:Sde_0084
OrganismSaccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) [Complete proteome] [HAMAP]
Taxonomic identifier203122 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeSaccharophagus

Protein attributes

Sequence length831 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929Tat-type signal Potential
Chain30 – 831802Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_0000256076

Regions

Domain41 – 97574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding481Iron-sulfur (4Fe-4S) By similarity
Metal binding511Iron-sulfur (4Fe-4S) By similarity
Metal binding551Iron-sulfur (4Fe-4S) By similarity
Metal binding831Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q21PN1 [UniParc].

Last modified April 18, 2006. Version 1.
Checksum: 8B6EA1C70B7AF770

FASTA83193,164
        10         20         30         40         50         60 
MTVTRRDFVR HQALATAAAA AGVAVPAAAT NIVTTAADNK LVWSKAPCRF CGTGCSVNVA 

        70         80         90        100        110        120 
TKEGRVVATH GDIKSPVNRG LNCVKGYFLS KVMYGEDRLT QPLLRKKNGV YAKDGEFEPV 

       130        140        150        160        170        180 
TWEEAFTIMA EKFKKTLKEK GPEGIGMFGS GQWTVWEGYA ASKLMKAGFR SNNIDPNARH 

       190        200        210        220        230        240 
CMASAVGGFM RTFGIDEPMG CYDDFEHADA FVLWGSNMAE MHPILWTRIA DRRLSYPHVQ 

       250        260        270        280        290        300 
VAVMSTYEHR SFDLADLGVV FTPQSDLAIA NFIANYIIQN KKVNWDFVKK HTNFRVGTTD 

       310        320        330        340        350        360 
IGYGLRPEHP LQKAAKNADS AGASEPIDFE TYAKFVADYT VEKASEISGV SEDKLIKLAK 

       370        380        390        400        410        420 
LYADPNIKVM SLWTMGVNQH TRGVWMNNLI YNIHLLTGKI SEPGNSPFSL TGQPSACGTA 

       430        440        450        460        470        480 
REVGTFSHRL PADMVVTNPK HRAYAEKIWK LPEGSIPEKV GAHAVLQSRK LKDGEINAYW 

       490        500        510        520        530        540 
VQVNNNVQAG PNINEEVLPG YRNPQNFIVV SDAYPTVTAQ AADLILPSAM WVEKEGAYGN 

       550        560        570        580        590        600 
AERRTQFWHQ LVNAPGDARS DLWQLVEFSK YFKVEEVWPA DLIAKMPEAK GKTLFDILYK 

       610        620        630        640        650        660 
NGKVNKFPLA QVSKDYENKE ADAFGFYIQK GLFEEYAEFG RGHGHDLADF DRYHEERGLR 

       670        680        690        700        710        720 
WPVVNGEETL WRYREGSDPY VEKGKGVQFY GKPDGKAIIF ALPYEPPAES PNKEYPFWLC 

       730        740        750        760        770        780 
TGRVIEHWHS GSMTQRVPEL YKAFPDAVCF MHPEDARSAG LRRGDKVKLQ SIRGHIITRI 

       790        800        810        820        830 
ETRGRNKPPK GLVFVPWFDA RQLINKVTLD ATDPISKQTD FKKCAVKVER V 

« Hide

References

[1]"Complete genome sequence of the complex carbohydrate-degrading marine bacterium, Saccharophagus degradans strain 2-40 T."
Weiner R.M., Taylor L.E. II, Henrissat B., Hauser L., Land M., Coutinho P.M., Rancurel C., Saunders E.H., Longmire A.G., Zhang H., Bayer E.A., Gilbert H.J., Larimer F., Zhulin I.B., Ekborg N.A., Lamed R., Richardson P.M., Borovok I., Hutcheson S.
PLoS Genet. 4:E1000087-E1000087(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 2-40 / ATCC 43961 / DSM 17024.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000282 Genomic DNA. Translation: ABD79348.1.
RefSeqYP_525560.1. NC_007912.1.

3D structure databases

ProteinModelPortalQ21PN1.
SMRQ21PN1. Positions 43-829.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING203122.Sde_0084.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD79348; ABD79348; Sde_0084.
GeneID3967323.
KEGGsde:Sde_0084.
PATRIC23398763. VBISacDeg56404_0090.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMALVYMHPE.
OrthoDBEOG6CVV7G.

Enzyme and pathway databases

BioCycSDEG203122:GI2M-84-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_SACD2
AccessionPrimary (citable) accession number: Q21PN1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: April 18, 2006
Last modified: May 14, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families