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Q21PN1

- NAPA_SACD2

UniProt

Q21PN1 - NAPA_SACD2

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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi48 – 481Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi55 – 551Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi83 – 831Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciSDEG203122:GI2M-84-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:Sde_0084
OrganismiSaccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)
Taxonomic identifieri203122 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeSaccharophagus
ProteomesiUP000001947: Chromosome

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Tat-type signalUniRule annotationAdd
BLAST
Chaini30 – 831802Periplasmic nitrate reductasePRO_0000256076Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi203122.Sde_0084.

Structurei

3D structure databases

ProteinModelPortaliQ21PN1.
SMRiQ21PN1. Positions 43-829.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 97574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiLVYMHPE.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q21PN1-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MTVTRRDFVR HQALATAAAA AGVAVPAAAT NIVTTAADNK LVWSKAPCRF
60 70 80 90 100
CGTGCSVNVA TKEGRVVATH GDIKSPVNRG LNCVKGYFLS KVMYGEDRLT
110 120 130 140 150
QPLLRKKNGV YAKDGEFEPV TWEEAFTIMA EKFKKTLKEK GPEGIGMFGS
160 170 180 190 200
GQWTVWEGYA ASKLMKAGFR SNNIDPNARH CMASAVGGFM RTFGIDEPMG
210 220 230 240 250
CYDDFEHADA FVLWGSNMAE MHPILWTRIA DRRLSYPHVQ VAVMSTYEHR
260 270 280 290 300
SFDLADLGVV FTPQSDLAIA NFIANYIIQN KKVNWDFVKK HTNFRVGTTD
310 320 330 340 350
IGYGLRPEHP LQKAAKNADS AGASEPIDFE TYAKFVADYT VEKASEISGV
360 370 380 390 400
SEDKLIKLAK LYADPNIKVM SLWTMGVNQH TRGVWMNNLI YNIHLLTGKI
410 420 430 440 450
SEPGNSPFSL TGQPSACGTA REVGTFSHRL PADMVVTNPK HRAYAEKIWK
460 470 480 490 500
LPEGSIPEKV GAHAVLQSRK LKDGEINAYW VQVNNNVQAG PNINEEVLPG
510 520 530 540 550
YRNPQNFIVV SDAYPTVTAQ AADLILPSAM WVEKEGAYGN AERRTQFWHQ
560 570 580 590 600
LVNAPGDARS DLWQLVEFSK YFKVEEVWPA DLIAKMPEAK GKTLFDILYK
610 620 630 640 650
NGKVNKFPLA QVSKDYENKE ADAFGFYIQK GLFEEYAEFG RGHGHDLADF
660 670 680 690 700
DRYHEERGLR WPVVNGEETL WRYREGSDPY VEKGKGVQFY GKPDGKAIIF
710 720 730 740 750
ALPYEPPAES PNKEYPFWLC TGRVIEHWHS GSMTQRVPEL YKAFPDAVCF
760 770 780 790 800
MHPEDARSAG LRRGDKVKLQ SIRGHIITRI ETRGRNKPPK GLVFVPWFDA
810 820 830
RQLINKVTLD ATDPISKQTD FKKCAVKVER V
Length:831
Mass (Da):93,164
Last modified:April 18, 2006 - v1
Checksum:i8B6EA1C70B7AF770
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000282 Genomic DNA. Translation: ABD79348.1.
RefSeqiWP_011466572.1. NC_007912.1.
YP_525560.1. NC_007912.1.

Genome annotation databases

EnsemblBacteriaiABD79348; ABD79348; Sde_0084.
GeneIDi3967323.
KEGGisde:Sde_0084.
PATRICi23398763. VBISacDeg56404_0090.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000282 Genomic DNA. Translation: ABD79348.1 .
RefSeqi WP_011466572.1. NC_007912.1.
YP_525560.1. NC_007912.1.

3D structure databases

ProteinModelPortali Q21PN1.
SMRi Q21PN1. Positions 43-829.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 203122.Sde_0084.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABD79348 ; ABD79348 ; Sde_0084 .
GeneIDi 3967323.
KEGGi sde:Sde_0084.
PATRICi 23398763. VBISacDeg56404_0090.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi LVYMHPE.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci SDEG203122:GI2M-84-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 2-40 / ATCC 43961 / DSM 17024.

Entry informationi

Entry nameiNAPA_SACD2
AccessioniPrimary (citable) accession number: Q21PN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: April 18, 2006
Last modified: November 26, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3