Q21N29 (METE_SACD2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	Sde_0638

Organism

Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) [Complete proteome] [HAMAP]

Taxonomic identifier

203122 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Saccharophagus

Protein attributes

Sequence length	765 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 765	765	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000017269
Sites
	Metal binding	651	1	Zinc By similarity
	Metal binding	653	1	Zinc By similarity
	Metal binding	736	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q21N29 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: 5B0116A9ABB3BA6F	FASTA	765	85,449
	10 20 30 40 50 60 MTTVHNLGFP RIGAKRELKF ACEAYWRGEI DQTQLDKTAH TLRANHWQTQ LDAGVEWLPV 70 80 90 100 110 120 GDFSYYDHVL DTSFLVGNIP VRAQADGQSK LDTYFKVARG TAKEAAGASC CGGSHAAEMT 130 140 150 160 170 180 KWFDTNYHYL VPELNANTHF KLNASKLLTE LEQARALTSK VKPVILGPLS YLWLAKITDG 190 200 210 220 230 240 STHKLGFLDD LITVYRELLG TLAKEAVEWV QFDEPILALD LPEQWQHAFE RVYHQLQLPN 250 260 270 280 290 300 LSVLLATYFG PLDTNLRLAA RLPTAALHLD ITRGGDDLPR VLDILSPHKI LSVGVIDGRN 310 320 330 340 350 360 IWKADLNKLL AQLKPVKERL GNGLWLAPSC SLLHTPVDLD SEEALDADIK NWLAFAKQKL 370 380 390 400 410 420 QELNILQRAL DNGETSVAEA LVHNAGAIAA RKASAKVNNA KVQSLVNNIS PSLSERESPF 430 440 450 460 470 480 ATRIEKQQAK YNLPTLPTTT IGSFPQTSEI RKNRKAFKSG EISQQQYDRT LRAEIAHAIE 490 500 510 520 530 540 LQEKIGIDVL VHGEAERNDM VEYFGEQLDG YVFTQFGWVQ SYGSRCVKPP IIFGDVSRPR 550 560 570 580 590 600 KITVDWAEYA QSLTSKPVKG MLTGPITMLQ WSFVRDDQPR KITAQQIALA LRSEVNDLEA 610 620 630 640 650 660 AGITIIQVDE PALREGLPLR KKDWQNYLDW AVEAFKISTA GVGDTTQIHT HMCYAEFNDI 670 680 690 700 710 720 IQAIADLDAD VITIETSRSN MELLSAFEQF NYPNDIGPGV YDIHSPNIPA TEQVVGLIKK 730 740 750 760 AAENLPLQRL WINPDCGLKT RKWEDVIPAL ENMVNAAKQL RAEVA

References

[1]

"Complete genome sequence of the complex carbohydrate-degrading marine bacterium, Saccharophagus degradans strain 2-40 T." Weiner R.M., Taylor L.E. II, Henrissat B., Hauser L., Land M., Coutinho P.M., Rancurel C., Saunders E.H., Longmire A.G., Zhang H., Bayer E.A., Gilbert H.J., Larimer F., Zhulin I.B., Ekborg N.A., Lamed R., Richardson P.M., Borovok I., Hutcheson S. PLoS Genet. 4:E1000087-E1000087(2008) [PubMed: 18516288] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 2-40 / ATCC 43961 / DSM 17024.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000282 Genomic DNA. Translation: ABD79900.1.
RefSeq	YP_526112.1. NC_007912.1.
3D structure databases
ProteinModelPortal	Q21N29.
ModBase	Search...
Protein-protein interaction databases
STRING	Q21N29.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3964967.
GenomeReviews	Gene locus Sde_0638 in contig CP000282_GR.
KEGG	sde:Sde_0638.
NMPDR	fig|203122.1.peg.384.
PATRIC	23400001. VBISacDeg56404_0705.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0620.
HOGENOM	HBG287495.
OMA	RNIWRAN.
PhylomeDB	Q21N29.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	SDEG203122:SDE_0638-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_SACD2

Accession

Primary (citable) accession number: Q21N29

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 18, 2006
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program