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Q21MG7 (DDL_SACD2) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Sde_0850
OrganismSaccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) [Complete proteome] [HAMAP]
Taxonomic identifier203122 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeSaccharophagus

Protein attributes

Sequence length314 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 314314D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000030489

Regions

Domain115 – 310196ATP-grasp
Nucleotide binding141 – 19656ATP By similarity

Sites

Metal binding2641Magnesium or manganese 1 By similarity
Metal binding2771Magnesium or manganese 1 By similarity
Metal binding2771Magnesium or manganese 2 By similarity
Metal binding2791Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q21MG7 [UniParc].

Last modified April 18, 2006. Version 1.
Checksum: A8791F34EF8907D1

FASTA31433,574
        10         20         30         40         50         60 
MTTTYVKKFD GNTAALGRTL VLFGGTSSER EVSLRSGAAV LQALQAGGVD AHGLDVQADA 

        70         80         90        100        110        120 
IAKIEAFAPD RVFIALHGPG GEDGKMQAVL DWLAIPYTGS DHAASALAMD KLKTKQVWQS 

       130        140        150        160        170        180 
VGLVTPEYAS LVENSDWQAV LDSLGGQGFV KPAHEGSSIG MSVVSTAQEL KAAYEKAAHY 

       190        200        210        220        230        240 
DAKVLVERRI VGREFTVAVL NGVALPAIGL KTDHVFYDYD AKYVSNTTQY LCPCGLTQEK 

       250        260        270        280        290        300 
EAELQSIAQQ AFAAVGCKGW GRVDFMQDEA GNFYLLEVNT VPGMTDHSLV PMAARQSGIE 

       310 
FNELVLEILA QTLV 

« Hide

References

[1]"Complete genome sequence of the complex carbohydrate-degrading marine bacterium, Saccharophagus degradans strain 2-40 T."
Weiner R.M., Taylor L.E. II, Henrissat B., Hauser L., Land M., Coutinho P.M., Rancurel C., Saunders E.H., Longmire A.G., Zhang H., Bayer E.A., Gilbert H.J., Larimer F., Zhulin I.B., Ekborg N.A., Lamed R., Richardson P.M., Borovok I., Hutcheson S.
PLoS Genet. 4:E1000087-E1000087(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 2-40 / ATCC 43961 / DSM 17024.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000282 Genomic DNA. Translation: ABD80112.1.
RefSeqYP_526324.1. NC_007912.1.

3D structure databases

ProteinModelPortalQ21MG7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING203122.Sde_0850.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD80112; ABD80112; Sde_0850.
GeneID3965199.
KEGGsde:Sde_0850.
PATRIC23400461. VBISacDeg56404_0934.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMADTQYRIP.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycSDEG203122:GI2M-856-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_SACD2
AccessionPrimary (citable) accession number: Q21MG7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 18, 2006
Last modified: February 19, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways