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Q21M30 (GLGB_SACD2) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:Sde_0987
OrganismSaccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) [Complete proteome] [HAMAP]
Taxonomic identifier203122 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeSaccharophagus

Protein attributes

Sequence length729 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7297291,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000260695

Sites

Active site4091Nucleophile By similarity
Active site4621Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q21M30 [UniParc].

Last modified April 18, 2006. Version 1.
Checksum: 958A76433E6BB7BF

FASTA72983,408
        10         20         30         40         50         60 
MLSITPEIIK EIVQGEYHDV FAVLGPHAQK NGYVIRAFFP AAKTLQVKSK LDGSVLAEAV 

        70         80         90        100        110        120 
MRNEEGFFEA NCNCSEKPSY YFTVGYGDKE FDVEDMYRFG STIPEGDLYL FGEGTYEQAY 

       130        140        150        160        170        180 
RLFGAHPTEV DGVPGCRFTV WAPNAKTVAV VGDFNFWQGR AHVMRKHIPS GIWELFIPYL 

       190        200        210        220        230        240 
GEGALYKYEI RNHSGDCLPH KADPYGFAAQ KPPEQASVVS VLDRYEWNDA EWFAKANNWT 

       250        260        270        280        290        300 
QRDRPISIYE VHLGSWKRVV EEDNRYLSYK ELAADLIPYV KSMGFTHIQL MPISEFPFDG 

       310        320        330        340        350        360 
SWGYQPVGLY APTSRFGSPE DFKYFVDCCH QNDLAVLIDW VPGHFPTDSH GLGLFDGTPL 

       370        380        390        400        410        420 
YEHADSRQGF HPDWNTYIYN YGRHEVKSFL MANALFWLEQ YHIDGLRVDA VASMLYLDYS 

       430        440        450        460        470        480 
RKDGEWLPNS YGGRENLEAI DFLRLVNERV YKRFPHAMMV AEESTAWPGV SSPTSCGGLG 

       490        500        510        520        530        540 
FGYKWNMGWM NDSLQYISKE PIHRQYHHHD MTFSLHYAFS ENFVLPLSHD EVVHGKRSLL 

       550        560        570        580        590        600 
GRMPGDAWQQ FANLRAYYAF MWTHPGKKLL FMGGEFAQGM EWNHDTSLSW HQLEIDYHSG 

       610        620        630        640        650        660 
IQTLVKSLNR LYTDVPALYK EDCMSSGFEW VEADDRHNSI FAFLRKAKDE KPVLVVANFT 

       670        680        690        700        710        720 
PVAREGYRVG VNQPGYYREL LNTDSELFGG SNLGNEGGVH SEEIAWHQRP QSVSINIPAL 


AAVVFQLDS 

« Hide

References

[1]"Complete genome sequence of the complex carbohydrate-degrading marine bacterium, Saccharophagus degradans strain 2-40 T."
Weiner R.M., Taylor L.E. II, Henrissat B., Hauser L., Land M., Coutinho P.M., Rancurel C., Saunders E.H., Longmire A.G., Zhang H., Bayer E.A., Gilbert H.J., Larimer F., Zhulin I.B., Ekborg N.A., Lamed R., Richardson P.M., Borovok I., Hutcheson S.
PLoS Genet. 4:E1000087-E1000087(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 2-40 / ATCC 43961 / DSM 17024.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000282 Genomic DNA. Translation: ABD80249.1.
RefSeqYP_526461.1. NC_007912.1.

3D structure databases

ProteinModelPortalQ21M30.
SMRQ21M30. Positions 117-729.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING203122.Sde_0987.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD80249; ABD80249; Sde_0987.
GeneID3966229.
KEGGsde:Sde_0987.
PATRIC23400761. VBISacDeg56404_1079.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAISEGRHE.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycSDEG203122:GI2M-998-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_SACD2
AccessionPrimary (citable) accession number: Q21M30
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: April 18, 2006
Last modified: June 11, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries