Reviewed, UniProtKB/Swiss-Prot Q21LY9 (DNAE2_SACD2)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	Sde_1028

Organism

Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) [Complete proteome] [HAMAP]

Taxonomic identifier

203122 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Saccharophagus

Protein attributes

Sequence length	1029 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1029	1029	Error-prone DNA polymerase HAMAP MF_01902		PRO_1000073691

Sequences

	Sequence		Length	Mass (Da)
	Q21LY9-1 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: 32D7E9BDFC895488	FASTA	1,029	115,435
	10 20 30 40 50 60 MQYTQLQTLT NYSFLKSASH PQELVEEAKI LGYHALAITD ECSLAGIVKA HVAAKELNLK 70 80 90 100 110 120 LLVGSYFELT NGFKIIAIAP NRQAYAELSG FISLARRRAS KGEYEAHLSD LRFRLQQCLI 130 140 150 160 170 180 IWLPYFNNHI SDVDVTTLAT AFKQRLWIGV SHTLIAAEQR LFSHLNKLAN ALHVPLVASG 190 200 210 220 230 240 LTYMHNKNCK PLLDILTAIR ENTPIQQLGT RLHSNAEVNL KPLHELNQLY PEALIQQTQV 250 260 270 280 290 300 IAQLCNFSLD ELRYQYPKEL VPSNTTPIAH LKKLVKEGEA KRWPQGTPEH AQKIIAMELG 310 320 330 340 350 360 LIEEMQYEYY FLTVHDIVHF ARSKNILCQG RGSAANSVVC YCLFITEIAP GQINVLFERF 370 380 390 400 410 420 ISKERNEPPD IDVDFEHQRR EEVIQYIYQK YGRERAALAA TVITYRSRSA IRDVGKAMGL 430 440 450 460 470 480 EAGLVGQLAK SLAWWDRTGD LIKRMESFGL NPETQKTMQH FFALVQQILG FPRHLSQHVG 490 500 510 520 530 540 GFIITQDKVS DLVPLENASM PDRTIIQWDK YDIEAMGLLK VDVLALGMLT ALRKSLETVS 550 560 570 580 590 600 QYDAAVYSLA TIPREDPATY AMLSKGDSIG VFQVESRAQM SMLPRLRPKC FYDLVIEIAI 610 620 630 640 650 660 VRPGPIQGDM VHPYLRRRDG IEEVHYQNDK IKSVLEPTLG IPIFQEQAIR LAMVAADFSG 670 680 690 700 710 720 GEADQLRRAM ASWGKNGSLL KFEDKFIQGM LNNGYPLDFA HRLFEQIKGF GGYGFPESHS 730 740 750 760 770 780 ASFALLCYAS SWLKCHHPAA FYCALLNSQP MGFYSASQLI QDARRHKVVV LPVEVNASGY 790 800 810 820 830 840 ESHVVLTNHN TSAPPNIIQL GLHMIKGLSI LTAERIVLAK GDKPFTTLKE LSLRAQLSSA 850 860 870 880 890 900 DLQLLASADA LHKLTGNRHN SRWQAAALMP HSPLLDGAEL EEDALNTPAP SIEKNIQTDF 910 920 930 940 950 960 NSTGLSLRLH PMALLRAQQP FNRCKKQSEL ASIHNGGFAQ VAGLVTGRQR PGTAKGTLFL 970 980 990 1000 1010 1020 TLEDETGNIN IVVWTSTQER CRQALLTAKL LLVKGRLETK DNVTHIIAGQ MFDYSHMLSE FDIKSRDFH

References

[1]

"Complete genome sequence of the complex carbohydrate-degrading marine bacterium, Saccharophagus degradans strain 2-40 T." Weiner R.M., Taylor L.E. II, Henrissat B., Hauser L., Land M., Coutinho P.M., Rancurel C., Saunders E.H., Longmire A.G., Zhang H., Bayer E.A., Gilbert H.J., Larimer F., Zhulin I.B., Ekborg N.A., Lamed R., Richardson P.M., Borovok I., Hutcheson S. PLoS Genet. 4:E1000087-E1000087(2008) [PubMed: 18516288] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000282 Genomic DNA. Translation: ABD80290.1.
RefSeq	YP_526502.1.
3D structure databases
SMR	Q21LY9. Positions 2-869.
ModBase	Search...
Protein-protein interaction databases
STRING	Q21LY9.
Genome annotation databases
GeneID	3967782.
GenomeReviews	Gene locus Sde_1028 in contig CP000282_GR.
KEGG	sde:Sde_1028.
NMPDR	fig|203122.1.peg.3184.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0587.
HOGENOM	HBG734490.
OMA	MGFYSPD.
Enzyme and pathway databases
BioCyc	SDEG203122:SDE_1028-MONOMER.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR016195. Pol/histidinol_Pase-like. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_SACD2

Accession

Primary (citable) accession number: Q21LY9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	April 18, 2006
Last modified:	February 9, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)