Reviewed, UniProtKB/Swiss-Prot Q21IQ9 (CHEB1_SACD2)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase 1
    EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	Sde_2160

Organism

Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) [Complete proteome] [HAMAP]

Taxonomic identifier

203122 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Saccharophagus

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity.
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm. HAMAP MF_00099
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity.
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	Chemotaxis response regulator protein-glutamate methylesterase 1 HAMAP MF_00099		PRO_0000264315
Regions
	Domain	4 – 121	118	Response regulatory
	Domain	177 – 365	189	CheB-type methylesterase
Sites
	Active site	189	1	By similarity
	Active site	216	1	By similarity
	Active site	310	1	By similarity
Amino acid modifications
	Modified residue	55	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q21IQ9-1 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: 94EB70803D47D30B	FASTA	365	38,984
	10 20 30 40 50 60 MAYKVLVVDD SQFFQVRLKQ IINEHPDLEV VGIAANGQEA IDLEESLRPD IISMDYEMPH 70 80 90 100 110 120 LDGISAVRSI LSKRPIPIVM FSSMTYEGAT ITLEALDAGA VDFIPKNFAE VSRDSVVLKK 130 140 150 160 170 180 RLHEKLLLFA QKAKPSVAAR STTQPSGVRP SALGANLSSS RSPRPASSAP SAPKKRIKGL 190 200 210 220 230 240 VKLVAIGAST GGPVAVSEII TRLPANFPVP VIVAQHMPEN FTKAFSERLN RQSAVEVREA 250 260 270 280 290 300 QDGDLLKSGV VYVAPGGNQL MVDKTGRSIR ILDGDARLTY KPSVDVLFAS AASAMGDKVL 310 320 330 340 350 360 AIVLTGMGAD GCDGAKLLKQ KGATIWGQDK DSCVVYGMPA AVAKAGLTDE VLPLDQVWQR LVSDV

References

[1]

"Complete sequence of Saccharophagus degradans 2-40." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Kyrpides N., Lykidis A., Richardson P., Weiner R. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000282 Genomic DNA. Translation: ABD81420.1.
RefSeq	YP_527632.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q21IQ9.
Genome annotation databases
GeneID	3967544.
GenomeReviews	Gene locus Sde_2160 in contig CP000282_GR.
KEGG	sde:Sde_2160.
NMPDR	fig|203122.1.peg.1125.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q21IQ9.
OMA	ETIGANK.
Enzyme and pathway databases
BioCyc	SDEG203122:SDE_2160-MON.
Family and domain databases
HAMAP	MF_00099. [Tree]
InterPro	IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
ProDom	PD005328. CheB_methylest. 1 hit. PD000039. Response_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_SACD2

Accession

Primary (citable) accession number: Q21IQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	April 18, 2006
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)