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Q21HK3

- SYI_SACD2

UniProt

Q21HK3 - SYI_SACD2

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Protein
Isoleucine--tRNA ligase
Gene
ileS, Sde_2566
Organism
Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei559 – 5591Aminoacyl-adenylate By similarity
Binding sitei603 – 6031ATP By similarity
Metal bindingi895 – 8951Zinc By similarity
Metal bindingi898 – 8981Zinc By similarity
Metal bindingi915 – 9151Zinc By similarity
Metal bindingi918 – 9181Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSDEG203122:GI2M-2611-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Sde_2566
OrganismiSaccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)
Taxonomic identifieri203122 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeSaccharophagus
ProteomesiUP000001947: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 932932Isoleucine--tRNA ligaseUniRule annotation
PRO_1000022114Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi203122.Sde_2566.

Structurei

3D structure databases

ProteinModelPortaliQ21HK3.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotation
Add
BLAST
Motifi600 – 6045"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q21HK3-1 [UniParc]FASTAAdd to Basket

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MTDYKATLNL PQTSFAMKAN LAQREPGMLK QWQQSKLYEQ IREARKGREQ    50
FILHDGPPYA NGDIHIGHAV NKILKDIIIK AKTLSGFDAP YVPGWDCHGL 100
PIEHNVEKKV GKAGVKIDHV AFRQKCRDYA RKQVEGQKKD FIRLGVLGEW 150
DTPYLTMDFK TEADTVRALG KIVENGHLHK GFKPVYWSVV GASALAEAEV 200
EYQDKTSFAI DVCFGVADVE DFAARVGSVA GTGNISVVIW TTTPWTLPAN 250
QAVSVNGELD YVVVQHGEQR LVLAEALVES VAKRLGVEEL ATVATFKGAA 300
IEGLKLNHPF YAKQVPVLLG DHVTTDAGTG CVHTAPDHGA DDFVVSNKYG 350
IETLNYVDEN GIYRDNVEIF AGDHVYKVDE KVIGLLEENG RLLHQAKLVH 400
SFPHCWRTKT PLIFRATPQW FVSMTKNNLL KDVKQAVEGV NWVPDWGRAR 450
IDSMLDSSPD WCISRQRTWG VPITLFVHKE TQELHPNTAA LVEDVAKRIE 500
EKGMDAWFEL DAVELLGDEA DQYQKVTDTL DVWFDSGVTH YSVVNARENL 550
RYPADLYLEG SDQHRGWFQS SLKTSMAING SAPYKQVLTH GFTVDADGKK 600
MSKSIGNTVS PQKVMNDLGA DVLRLWVAAT DFSGDMSVSD EILKRTADSY 650
RRIRNTARFF LSNLTGFNPQ TDLLPAEEML SLDRWAVDRA AALQADILKS 700
YDTYQLHQIY QKLHNFCVVE MGGFYLDIIK DRQYTTKETS HARRSAQSAL 750
YHIVEAFVRW IAPICSFTAD EIWQAMPGEK TGTVFTAEWY NLPRLAEGAE 800
LGNSYWQFIA KVKTAVNKTI EAKRSAGEVG GSLAAEVTLY CSESVAQKLT 850
LLKDELRFVL ICSSVTVVAA SETEGEATEV EGLRVAVAKS SHEKCARCWH 900
HREDVGQNTA HPEICGRCVE NIDGEGESRE FA 932
Length:932
Mass (Da):104,058
Last modified:April 18, 2006 - v1
Checksum:i96EA5D8EC7A224C6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000282 Genomic DNA. Translation: ABD81826.1.
RefSeqiWP_011469043.1. NC_007912.1.
YP_528038.1. NC_007912.1.

Genome annotation databases

EnsemblBacteriaiABD81826; ABD81826; Sde_2566.
GeneIDi3968675.
KEGGisde:Sde_2566.
PATRICi23404196. VBISacDeg56404_2763.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000282 Genomic DNA. Translation: ABD81826.1 .
RefSeqi WP_011469043.1. NC_007912.1.
YP_528038.1. NC_007912.1.

3D structure databases

ProteinModelPortali Q21HK3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 203122.Sde_2566.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABD81826 ; ABD81826 ; Sde_2566 .
GeneIDi 3968675.
KEGGi sde:Sde_2566.
PATRICi 23404196. VBISacDeg56404_2763.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SDEG203122:GI2M-2611-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 2-40 / ATCC 43961 / DSM 17024.

Entry informationi

Entry nameiSYI_SACD2
AccessioniPrimary (citable) accession number: Q21HK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 18, 2006
Last modified: September 3, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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