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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei559 – 5591Aminoacyl-adenylateUniRule annotation
Binding sitei603 – 6031ATPUniRule annotation
Metal bindingi895 – 8951ZincUniRule annotation
Metal bindingi898 – 8981ZincUniRule annotation
Metal bindingi915 – 9151ZincUniRule annotation
Metal bindingi918 – 9181ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSDEG203122:GI2M-2611-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Sde_2566
OrganismiSaccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)
Taxonomic identifieri203122 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeSaccharophagus
ProteomesiUP000001947 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 932932Isoleucine--tRNA ligasePRO_1000022114Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi203122.Sde_2566.

Structurei

3D structure databases

ProteinModelPortaliQ21HK3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi600 – 6045"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q21HK3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDYKATLNL PQTSFAMKAN LAQREPGMLK QWQQSKLYEQ IREARKGREQ
60 70 80 90 100
FILHDGPPYA NGDIHIGHAV NKILKDIIIK AKTLSGFDAP YVPGWDCHGL
110 120 130 140 150
PIEHNVEKKV GKAGVKIDHV AFRQKCRDYA RKQVEGQKKD FIRLGVLGEW
160 170 180 190 200
DTPYLTMDFK TEADTVRALG KIVENGHLHK GFKPVYWSVV GASALAEAEV
210 220 230 240 250
EYQDKTSFAI DVCFGVADVE DFAARVGSVA GTGNISVVIW TTTPWTLPAN
260 270 280 290 300
QAVSVNGELD YVVVQHGEQR LVLAEALVES VAKRLGVEEL ATVATFKGAA
310 320 330 340 350
IEGLKLNHPF YAKQVPVLLG DHVTTDAGTG CVHTAPDHGA DDFVVSNKYG
360 370 380 390 400
IETLNYVDEN GIYRDNVEIF AGDHVYKVDE KVIGLLEENG RLLHQAKLVH
410 420 430 440 450
SFPHCWRTKT PLIFRATPQW FVSMTKNNLL KDVKQAVEGV NWVPDWGRAR
460 470 480 490 500
IDSMLDSSPD WCISRQRTWG VPITLFVHKE TQELHPNTAA LVEDVAKRIE
510 520 530 540 550
EKGMDAWFEL DAVELLGDEA DQYQKVTDTL DVWFDSGVTH YSVVNARENL
560 570 580 590 600
RYPADLYLEG SDQHRGWFQS SLKTSMAING SAPYKQVLTH GFTVDADGKK
610 620 630 640 650
MSKSIGNTVS PQKVMNDLGA DVLRLWVAAT DFSGDMSVSD EILKRTADSY
660 670 680 690 700
RRIRNTARFF LSNLTGFNPQ TDLLPAEEML SLDRWAVDRA AALQADILKS
710 720 730 740 750
YDTYQLHQIY QKLHNFCVVE MGGFYLDIIK DRQYTTKETS HARRSAQSAL
760 770 780 790 800
YHIVEAFVRW IAPICSFTAD EIWQAMPGEK TGTVFTAEWY NLPRLAEGAE
810 820 830 840 850
LGNSYWQFIA KVKTAVNKTI EAKRSAGEVG GSLAAEVTLY CSESVAQKLT
860 870 880 890 900
LLKDELRFVL ICSSVTVVAA SETEGEATEV EGLRVAVAKS SHEKCARCWH
910 920 930
HREDVGQNTA HPEICGRCVE NIDGEGESRE FA
Length:932
Mass (Da):104,058
Last modified:April 18, 2006 - v1
Checksum:i96EA5D8EC7A224C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000282 Genomic DNA. Translation: ABD81826.1.
RefSeqiWP_011469043.1. NC_007912.1.
YP_528038.1. NC_007912.1.

Genome annotation databases

EnsemblBacteriaiABD81826; ABD81826; Sde_2566.
KEGGisde:Sde_2566.
PATRICi23404196. VBISacDeg56404_2763.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000282 Genomic DNA. Translation: ABD81826.1.
RefSeqiWP_011469043.1. NC_007912.1.
YP_528038.1. NC_007912.1.

3D structure databases

ProteinModelPortaliQ21HK3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi203122.Sde_2566.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD81826; ABD81826; Sde_2566.
KEGGisde:Sde_2566.
PATRICi23404196. VBISacDeg56404_2763.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciSDEG203122:GI2M-2611-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 2-40 / ATCC 43961 / DSM 17024.

Entry informationi

Entry nameiSYI_SACD2
AccessioniPrimary (citable) accession number: Q21HK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 18, 2006
Last modified: April 29, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.