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Q21HK3

- SYI_SACD2

UniProt

Q21HK3 - SYI_SACD2

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (18 Apr 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei559 – 5591Aminoacyl-adenylateUniRule annotation
    Binding sitei603 – 6031ATPUniRule annotation
    Metal bindingi895 – 8951ZincUniRule annotation
    Metal bindingi898 – 8981ZincUniRule annotation
    Metal bindingi915 – 9151ZincUniRule annotation
    Metal bindingi918 – 9181ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciSDEG203122:GI2M-2611-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Sde_2566
    OrganismiSaccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)
    Taxonomic identifieri203122 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeSaccharophagus
    ProteomesiUP000001947: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 932932Isoleucine--tRNA ligasePRO_1000022114Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi203122.Sde_2566.

    Structurei

    3D structure databases

    ProteinModelPortaliQ21HK3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi600 – 6045"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q21HK3-1 [UniParc]FASTAAdd to Basket

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    MTDYKATLNL PQTSFAMKAN LAQREPGMLK QWQQSKLYEQ IREARKGREQ    50
    FILHDGPPYA NGDIHIGHAV NKILKDIIIK AKTLSGFDAP YVPGWDCHGL 100
    PIEHNVEKKV GKAGVKIDHV AFRQKCRDYA RKQVEGQKKD FIRLGVLGEW 150
    DTPYLTMDFK TEADTVRALG KIVENGHLHK GFKPVYWSVV GASALAEAEV 200
    EYQDKTSFAI DVCFGVADVE DFAARVGSVA GTGNISVVIW TTTPWTLPAN 250
    QAVSVNGELD YVVVQHGEQR LVLAEALVES VAKRLGVEEL ATVATFKGAA 300
    IEGLKLNHPF YAKQVPVLLG DHVTTDAGTG CVHTAPDHGA DDFVVSNKYG 350
    IETLNYVDEN GIYRDNVEIF AGDHVYKVDE KVIGLLEENG RLLHQAKLVH 400
    SFPHCWRTKT PLIFRATPQW FVSMTKNNLL KDVKQAVEGV NWVPDWGRAR 450
    IDSMLDSSPD WCISRQRTWG VPITLFVHKE TQELHPNTAA LVEDVAKRIE 500
    EKGMDAWFEL DAVELLGDEA DQYQKVTDTL DVWFDSGVTH YSVVNARENL 550
    RYPADLYLEG SDQHRGWFQS SLKTSMAING SAPYKQVLTH GFTVDADGKK 600
    MSKSIGNTVS PQKVMNDLGA DVLRLWVAAT DFSGDMSVSD EILKRTADSY 650
    RRIRNTARFF LSNLTGFNPQ TDLLPAEEML SLDRWAVDRA AALQADILKS 700
    YDTYQLHQIY QKLHNFCVVE MGGFYLDIIK DRQYTTKETS HARRSAQSAL 750
    YHIVEAFVRW IAPICSFTAD EIWQAMPGEK TGTVFTAEWY NLPRLAEGAE 800
    LGNSYWQFIA KVKTAVNKTI EAKRSAGEVG GSLAAEVTLY CSESVAQKLT 850
    LLKDELRFVL ICSSVTVVAA SETEGEATEV EGLRVAVAKS SHEKCARCWH 900
    HREDVGQNTA HPEICGRCVE NIDGEGESRE FA 932
    Length:932
    Mass (Da):104,058
    Last modified:April 18, 2006 - v1
    Checksum:i96EA5D8EC7A224C6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000282 Genomic DNA. Translation: ABD81826.1.
    RefSeqiWP_011469043.1. NC_007912.1.
    YP_528038.1. NC_007912.1.

    Genome annotation databases

    EnsemblBacteriaiABD81826; ABD81826; Sde_2566.
    GeneIDi3968675.
    KEGGisde:Sde_2566.
    PATRICi23404196. VBISacDeg56404_2763.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000282 Genomic DNA. Translation: ABD81826.1 .
    RefSeqi WP_011469043.1. NC_007912.1.
    YP_528038.1. NC_007912.1.

    3D structure databases

    ProteinModelPortali Q21HK3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 203122.Sde_2566.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABD81826 ; ABD81826 ; Sde_2566 .
    GeneIDi 3968675.
    KEGGi sde:Sde_2566.
    PATRICi 23404196. VBISacDeg56404_2763.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci SDEG203122:GI2M-2611-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 2-40 / ATCC 43961 / DSM 17024.

    Entry informationi

    Entry nameiSYI_SACD2
    AccessioniPrimary (citable) accession number: Q21HK3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: April 18, 2006
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3