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Q21H65

- PNP_SACD2

UniProt

Q21H65 - PNP_SACD2

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Protein
Polyribonucleotide nucleotidyltransferase
Gene
pnp, Sde_2704
Organism
Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi498 – 4981Magnesium By similarity
Metal bindingi504 – 5041Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. RNA binding Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA processing Source: InterPro
  2. mRNA catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciSDEG203122:GI2M-2749-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:Sde_2704
OrganismiSaccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)
Taxonomic identifieri203122 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeSaccharophagus
ProteomesiUP000001947: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 722722Polyribonucleotide nucleotidyltransferaseUniRule annotation
PRO_0000329824Add
BLAST

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation By similarity.

Protein-protein interaction databases

STRINGi203122.Sde_2704.

Structurei

3D structure databases

ProteinModelPortaliQ21H65.
SMRiQ21H65. Positions 629-703.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini565 – 62460KH
Add
BLAST
Domaini634 – 70269S1 motif
Add
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OMAiPRWDWVA.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q21H65-1 [UniParc]FASTAAdd to Basket

« Hide

MQWGTPSQKD KKVNPVIKKF QYGNDTVTLE TGRIARQASG AVLASMGQTS    50
VLVAVTGSKE AKPGQDFFPL SVHYQEKAYA AGKIPGGFFK REARPSEKET 100
LTSRLIDRPI RPLFPNGFLN EVQVVCTVIS AEKDVDPDIV AMIGTSAALA 150
ISGIPFNGPI GAARVGYTQE SGYLLNPTYS TLKTSELDMV VAGTADAVLM 200
VESEAKELPE DIMLGAVLYA HQEMQAVVQA VAELAKDAAK PVWDWKPEEV 250
NAELKAKVEH GFADSVGVAY RITDKQKRYD RLSELRNQAV AELATEESGV 300
SADEVKGLFA KIEKQIVRSR VVAGEPRIDG RDSKTVRPLH VEVGVLPKVH 350
GSALFTRGET QALVVSTLGS ARDAQIIDAL EGERKDPFML HYNFPPYSVG 400
ECGRMGATGR REIGHGRLAR RGIGAVLPKE EDFPYTMRVV SEITESNGSS 450
SMASVCGASL ALMDAGVPLK APVAGIAMGL VKEDNGFAVL TDILGDEDHL 500
GDMDFKVAGT ADGVTALQMD IKIEGITEEI MEIALGQAMD ARLHILAEMN 550
RVIASPRKEL SENAPQFHTM KIDPDKIRDI IGKGGATIRS ITEETGASID 600
IDDNGTIKIY ADDGDGMQAA IARIEEITAE AEIGAVYQGK VVRIVDFGAF 650
VNFLPGKDGL VHISQIAHER VQNVSDYLKE GQDIEVKCMD IDQRGRIKLS 700
IKELLPAPEA ETAEAPAGEG EE 722
Length:722
Mass (Da):77,810
Last modified:April 18, 2006 - v1
Checksum:i026FCB55E7C644BD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000282 Genomic DNA. Translation: ABD81964.1.
RefSeqiWP_011469181.1. NC_007912.1.
YP_528176.1. NC_007912.1.

Genome annotation databases

EnsemblBacteriaiABD81964; ABD81964; Sde_2704.
GeneIDi3968446.
KEGGisde:Sde_2704.
PATRICi23404494. VBISacDeg56404_2912.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000282 Genomic DNA. Translation: ABD81964.1 .
RefSeqi WP_011469181.1. NC_007912.1.
YP_528176.1. NC_007912.1.

3D structure databases

ProteinModelPortali Q21H65.
SMRi Q21H65. Positions 629-703.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 203122.Sde_2704.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABD81964 ; ABD81964 ; Sde_2704 .
GeneIDi 3968446.
KEGGi sde:Sde_2704.
PATRICi 23404494. VBISacDeg56404_2912.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218327.
KOi K00962.
OMAi PRWDWVA.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci SDEG203122:GI2M-2749-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 2-40 / ATCC 43961 / DSM 17024.

Entry informationi

Entry nameiPNP_SACD2
AccessioniPrimary (citable) accession number: Q21H65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 18, 2006
Last modified: September 3, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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