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Q21FG6 (SYL_SACD2) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Sde_3308
OrganismSaccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) [Complete proteome] [HAMAP]
Taxonomic identifier203122 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeSaccharophagus

Protein attributes

Sequence length819 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 819819Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009421

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif577 – 5815"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5801ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q21FG6 [UniParc].

Last modified April 18, 2006. Version 1.
Checksum: 513F6286CAE6CF0C

FASTA81992,026
        10         20         30         40         50         60 
MNEYYQPSEI EAQAQKYWEE NKSFNVTEDP TKEKFYCLAM FPYPSGRLHM GHVRNYTISD 

        70         80         90        100        110        120 
VISRFHRMQG KNVLHPMGWD AFGLPAENAA IKNNTAPAKW TYSNTDYMRK QLTELGFGFD 

       130        140        150        160        170        180 
WSREVTTCKP DYYKWEQWFF TRLYEKGLAY KKVASVNWCP NDQTVLANEQ VVDGQCWRCD 

       190        200        210        220        230        240 
TAVERKEIPQ WFIRITDYAE ELLADLDKLP NWPEQVKTMQ RNWIGKSQGV EMRFDLANPI 

       250        260        270        280        290        300 
AGTTGFDVYT TRPDTLMGVT YVSLAAEHPI AKALAETNPA LAAFIQECKV QSVAEADMAN 

       310        320        330        340        350        360 
MEKKGIDTGI KAKHPITGDE VSVWVANYVL MDYGSGAVMA VPAHDQRDWE FAKKYDLEIK 

       370        380        390        400        410        420 
QVIAPEDGSD IDLTKEAFVD KGVLVNSGEY DGLNFNAAFE AISQTLQAAN KGKVTTNFRL 

       430        440        450        460        470        480 
RDWGVSRQRY WGSPIPIFNL PDGGVIPVPA DRLPVLLPED VQMDGVQSPI KADKEWCKAE 

       490        500        510        520        530        540 
LNGQAVEHET DTFDTFMESS WYYARYTSPN ADSMLDPDKA NYWLPVDQYV GGIEHAILHL 

       550        560        570        580        590        600 
LYARFFHKLM RDEGLVECDE PFERLLCQGM VLKDGTKMSK SKGNTVDPED LIKTYGADTV 

       610        620        630        640        650        660 
RLFSMFAAPP EQSLEWTDSG VEGAFRFLKK LWKAVASHLE AGSAGEIDAN SLDEQQKALR 

       670        680        690        700        710        720 
RKTHETISKV SDDYGRRQTF NTAIAAVMEL LNEITRSADR STPNGLAVER EALEAATLLL 

       730        740        750        760        770        780 
APIVPHACHA LWQAFGNEVA VLDAPWPTVD EAALVKDTIT IVAQVNGKVR AKLDAPANAD 

       790        800        810 
KDALEKIALA DESVLKHIDG KMIRKVIVVP GKLVNIVAN 

« Hide

References

[1]"Complete genome sequence of the complex carbohydrate-degrading marine bacterium, Saccharophagus degradans strain 2-40 T."
Weiner R.M., Taylor L.E. II, Henrissat B., Hauser L., Land M., Coutinho P.M., Rancurel C., Saunders E.H., Longmire A.G., Zhang H., Bayer E.A., Gilbert H.J., Larimer F., Zhulin I.B., Ekborg N.A., Lamed R., Richardson P.M., Borovok I., Hutcheson S.
PLoS Genet. 4:E1000087-E1000087(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 2-40 / ATCC 43961 / DSM 17024.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000282 Genomic DNA. Translation: ABD82563.1.
RefSeqYP_528775.1. NC_007912.1.

3D structure databases

ProteinModelPortalQ21FG6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING203122.Sde_3308.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD82563; ABD82563; Sde_3308.
GeneID3965819.
KEGGsde:Sde_3308.
PATRIC23405813. VBISacDeg56404_3567.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycSDEG203122:GI2M-3358-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_SACD2
AccessionPrimary (citable) accession number: Q21FG6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 18, 2006
Last modified: April 16, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries