ID   GSH1_SACD2              Reviewed;         530 AA.
AC   Q21EP1;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   18-APR-2006, sequence version 1.
DT   16-JUN-2009, entry version 20.
DE   RecName: Full=Glutamate--cysteine ligase;
DE            EC=6.3.2.2;
DE   AltName: Full=Gamma-glutamylcysteine synthetase;
DE   AltName: Full=Gamma-ECS;
DE            Short=GCS;
GN   Name=gshA; OrderedLocusNames=Sde_3583;
OS   Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Alteromonadaceae; Saccharophagus.
OX   NCBI_TaxID=203122;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Saunders E.H., Brettin T., Bruce D.,
RA   Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M.,
RA   Kyrpides N., Lykidis A., Richardson P., Weiner R.;
RT   "Complete sequence of Saccharophagus degradans 2-40.";
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: ATP + L-glutamate + L-cysteine = ADP +
CC       phosphate + gamma-L-glutamyl-L-cysteine.
CC   -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione
CC       from L-cysteine and L-glutamate: step 1/2.
CC   -!- SIMILARITY: Belongs to the glutamate--cysteine ligase type 1
CC       family. Type 1 subfamily.
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DR   EMBL; CP000282; ABD82838.1; -; Genomic_DNA.
DR   RefSeq; YP_529050.1; -.
DR   GeneID; 3966445; -.
DR   GenomeReviews; CP000282_GR; Sde_3583.
DR   KEGG; sde:Sde_3583; -.
DR   NMPDR; fig|203122.1.peg.1595; -.
DR   HOGENOM; Q21EP1; -.
DR   OMA; Q21EP1; DTLYLPY.
DR   BioCyc; SDEG203122:SDE_3583-MON; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004357; F:glutamate-cysteine ligase activity; IEA:HAMAP.
DR   GO; GO:0006750; P:glutathione biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00578; -; 1.
DR   InterPro; IPR007370; Glu_cys_ligase.
DR   InterPro; IPR006334; Glut_cys_ligase.
DR   Pfam; PF04262; Glu_cys_ligase; 1.
DR   TIGRFAMs; TIGR01434; glu_cys_ligase; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Glutathione biosynthesis; Ligase;
KW   Nucleotide-binding.
FT   CHAIN         1    530       Glutamate--cysteine ligase.
FT                                /FTId=PRO_1000025185.
SQ   SEQUENCE   530 AA;  60265 MW;  C55176AC2B39CD30 CRC64;
     MYEIQSFHAG LYDSKNAPVL TGIMRGIERE GLRIDRDGKL AQTPHPVALG SALTHPQITT
     DFSEALLEFI TPPTHRVEDL FKQLYDIQGY TLSKLDDELI WSSSMPCVLN DDATIPVAQY
     GSSNNGTMKT VYRVGLGHRY GRAMQTVAGL HYNFSLPDAF WSFLHREEYS LLDLQDFKDQ
     KYFALIRNFR RYYWLLVYLF GASPALCGSF IKGREHNLQP LIDERTLHLP YATSLRMGDL
     GYQSSAQESL YVCYNDKQSY ITTLCAAITT PIDEYKAIGL QDEKGEFKQL NTSLLQIENE
     FYSSIRPKRT AKHGETALSA LRNRGVEYIE VRCLDIDPFD PLGVNKPQVR FLDTFLLYCA
     LQDSPDTSPE ESANILRNQK TVVTEGRSPK ALIESFTKGK IPLKQAGEAL IKAMRPVAEL
     LDIAHETEEH TQSLETQLAA ILNPELTPSA RIISHLSAKK LPYAHYALAQ SRHHHETLLA
     KKPSNNTMQQ FEKMAAESLD KQTRLEQKNS GNFSEFLQEY YKQYQMCCDK
//