Q21EP1 (GSH1_SACD2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--cysteine ligase
EC=6.3.2.2

Alternative name(s):
Gamma-ECS
Short name=GCS
Gamma-glutamylcysteine synthetase

Gene names

Name:	gshA
Ordered Locus Names:	Sde_3583

Organism

Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) [Complete proteome] [HAMAP]

Taxonomic identifier

203122 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Saccharophagus

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine. HAMAP MF_00578
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. HAMAP MF_00578
Sequence similarities	Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutathione biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-cysteine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 530	530	Glutamate--cysteine ligase HAMAP MF_00578		PRO_1000025185

Sequences

	Sequence		Length	Mass (Da)
	Q21EP1 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: C55176AC2B39CD30	FASTA	530	60,265
	10 20 30 40 50 60 MYEIQSFHAG LYDSKNAPVL TGIMRGIERE GLRIDRDGKL AQTPHPVALG SALTHPQITT 70 80 90 100 110 120 DFSEALLEFI TPPTHRVEDL FKQLYDIQGY TLSKLDDELI WSSSMPCVLN DDATIPVAQY 130 140 150 160 170 180 GSSNNGTMKT VYRVGLGHRY GRAMQTVAGL HYNFSLPDAF WSFLHREEYS LLDLQDFKDQ 190 200 210 220 230 240 KYFALIRNFR RYYWLLVYLF GASPALCGSF IKGREHNLQP LIDERTLHLP YATSLRMGDL 250 260 270 280 290 300 GYQSSAQESL YVCYNDKQSY ITTLCAAITT PIDEYKAIGL QDEKGEFKQL NTSLLQIENE 310 320 330 340 350 360 FYSSIRPKRT AKHGETALSA LRNRGVEYIE VRCLDIDPFD PLGVNKPQVR FLDTFLLYCA 370 380 390 400 410 420 LQDSPDTSPE ESANILRNQK TVVTEGRSPK ALIESFTKGK IPLKQAGEAL IKAMRPVAEL 430 440 450 460 470 480 LDIAHETEEH TQSLETQLAA ILNPELTPSA RIISHLSAKK LPYAHYALAQ SRHHHETLLA 490 500 510 520 530 KKPSNNTMQQ FEKMAAESLD KQTRLEQKNS GNFSEFLQEY YKQYQMCCDK

References

[1]

"Complete genome sequence of the complex carbohydrate-degrading marine bacterium, Saccharophagus degradans strain 2-40 T." Weiner R.M., Taylor L.E. II, Henrissat B., Hauser L., Land M., Coutinho P.M., Rancurel C., Saunders E.H., Longmire A.G., Zhang H., Bayer E.A., Gilbert H.J., Larimer F., Zhulin I.B., Ekborg N.A., Lamed R., Richardson P.M., Borovok I., Hutcheson S. PLoS Genet. 4:E1000087-E1000087(2008) [PubMed: 18516288] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 2-40 / ATCC 43961 / DSM 17024.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000282 Genomic DNA. Translation: ABD82838.1.
RefSeq	YP_529050.1. NC_007912.1.
3D structure databases
ProteinModelPortal	Q21EP1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q21EP1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3966445.
GenomeReviews	Gene locus Sde_3583 in contig CP000282_GR.
KEGG	sde:Sde_3583.
NMPDR	fig|203122.1.peg.1595.
PATRIC	23406381. VBISacDeg56404_3851.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2918.
HOGENOM	HBG289023.
OMA	SANILRN.
PhylomeDB	Q21EP1.
ProtClustDB	CLSK2528914.
Enzyme and pathway databases
BioCyc	SDEG203122:SDE_3583-MONOMER.
Family and domain databases
HAMAP	MF_00578. Glu_cys_ligase. [Tree]
InterPro	IPR007370. Glu_cys_ligase. IPR006334. Glut_cys_ligase. [Graphical view]
KO	K01919.
Pfam	PF04262. Glu_cys_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01434. Glu_cys_ligase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GSH1_SACD2

Accession

Primary (citable) accession number: Q21EP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 18, 2006
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program