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Q21EN2 (TRMB_SACD2) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:Sde_3592
OrganismSaccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) [Complete proteome] [HAMAP]
Taxonomic identifier203122 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeSaccharophagus

Protein attributes

Sequence length229 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 229229tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288220

Regions

Region140 – 1456Interaction with RNA Potential
Region207 – 2104Substrate binding By similarity

Sites

Active site1341 By similarity
Binding site591S-adenosyl-L-methionine By similarity
Binding site841S-adenosyl-L-methionine By similarity
Binding site1111S-adenosyl-L-methionine By similarity
Binding site1341S-adenosyl-L-methionine By similarity
Binding site1381Substrate By similarity
Binding site1701Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q21EN2 [UniParc].

Last modified April 18, 2006. Version 1.
Checksum: C9406958EB2FBF16

FASTA22925,758
        10         20         30         40         50         60 
MQPEFKKKSI RSFVIRGGRM TEGQKNAFDK WWPHYGVSLF DGPINPAELF GRSAPLVLEI 

        70         80         90        100        110        120 
GFGMGDSLVD MAIADPDSDF IGIEVHPPGV GRLINRAGTE GVPNLRCYMA DANDVLSDCI 

       130        140        150        160        170        180 
PDGSLDRLQL FFPDPWHKKK HNKRRIVQPE FVEKIRAKLK IGGHFHMATD WEAYAEHMME 

       190        200        210        220 
VMTAAPGYEN IAGGSEFAPQ PSYRPTTKFE RRGENLGHGV WDIIFSRSL 

« Hide

References

[1]"Complete genome sequence of the complex carbohydrate-degrading marine bacterium, Saccharophagus degradans strain 2-40 T."
Weiner R.M., Taylor L.E. II, Henrissat B., Hauser L., Land M., Coutinho P.M., Rancurel C., Saunders E.H., Longmire A.G., Zhang H., Bayer E.A., Gilbert H.J., Larimer F., Zhulin I.B., Ekborg N.A., Lamed R., Richardson P.M., Borovok I., Hutcheson S.
PLoS Genet. 4:E1000087-E1000087(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 2-40 / ATCC 43961 / DSM 17024.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000282 Genomic DNA. Translation: ABD82847.1.
RefSeqYP_529059.1. NC_007912.1.

3D structure databases

ProteinModelPortalQ21EN2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING203122.Sde_3592.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD82847; ABD82847; Sde_3592.
GeneID3966454.
KEGGsde:Sde_3592.
PATRIC23406401. VBISacDeg56404_3861.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMALDKDHTI.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycSDEG203122:GI2M-3642-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_SACD2
AccessionPrimary (citable) accession number: Q21EN2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: April 18, 2006
Last modified: April 16, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways