ID   GFA_RHOPB               Reviewed;         189 AA.
AC   Q21D57;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   18-APR-2006, sequence version 1.
DT   16-JUN-2009, entry version 21.
DE   RecName: Full=Glutathione-dependent formaldehyde-activating enzyme;
DE            EC=4.4.1.22;
DE   AltName: Full=S-(hydroxymethyl)glutathione synthase;
GN   Name=gfa; OrderedLocusNames=RPC_0100;
OS   Rhodopseudomonas palustris (strain BisB18).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Bradyrhizobiaceae; Rhodopseudomonas.
OX   NCBI_TaxID=316056;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA   Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Pelletier D.A., Kyrpides N.,
RA   Anderson I., Oda Y., Harwood C.S., Richardson P.;
RT   "Complete sequence of Rhodopseudomonas palustris BisB18.";
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the condensation of formaldehyde and
CC       glutathione to S-hydroxymethylglutathione (By similarity).
CC   -!- CATALYTIC ACTIVITY: S-(hydroxymethyl)glutathione = glutathione +
CC       formaldehyde.
CC   -!- COFACTOR: Binds 2 zinc ions per subunit (By similarity).
CC   -!- PATHWAY: One-carbon metabolism; formaldehyde degradation; formate
CC       from formaldehyde (glutathione route): step 1/3.
CC   -!- SIMILARITY: Belongs to the gfa family.
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DR   EMBL; CP000301; ABD85679.1; -; Genomic_DNA.
DR   RefSeq; YP_529998.1; -.
DR   GeneID; 3971320; -.
DR   GenomeReviews; CP000301_GR; RPC_0100.
DR   KEGG; rpc:RPC_0100; -.
DR   HOGENOM; Q21D57; -.
DR   OMA; Q21D57; HACGCTK.
DR   BioCyc; RPAL316056:RPC_0100-MON; -.
DR   GO; GO:0051907; F:S-(hydroxymethyl)glutathione synthase activity; IEA:HAMAP.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0046294; P:formaldehyde catabolic process; IEA:HAMAP.
DR   HAMAP; MF_00723; -; 1.
DR   InterPro; IPR014185; Formald_GSH.
DR   InterPro; IPR006913; GFA.
DR   Pfam; PF04828; GFA; 1.
DR   PIRSF; PIRSF033318; Formald_GSH; 1.
DR   TIGRFAMs; TIGR02820; formald_GSH; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Lyase; Metal-binding; Zinc.
FT   CHAIN         1    189       Glutathione-dependent formaldehyde-
FT                                activating enzyme.
FT                                /FTId=PRO_1000045840.
FT   METAL        27     27       Zinc 1; structural (By similarity).
FT   METAL        29     29       Zinc 1; structural (By similarity).
FT   METAL        48     48       Zinc 2; catalytic (By similarity).
FT   METAL        50     50       Zinc 2; catalytic (By similarity).
FT   METAL        53     53       Zinc 2; catalytic (By similarity).
FT   METAL        95     95       Zinc 1; structural (By similarity).
FT   METAL        98     98       Zinc 1; structural (By similarity).
SQ   SEQUENCE   189 AA;  20028 MW;  26C883D531979A63 CRC64;
     MTVHIHPAVD QGVKQGSGSF AGGTLVCKCQ DRPVKVGIKG DVAHNHACGC TKCWKPDGAT
     FSVVAVVPRD NVTVLENGDK LHVVDASATI QRHACKVCGT HMYGRIENKS HPFYGLDFIH
     PELFQEGGSA APEFAAFVSS VIESGVDPSE MPGIRARLKE LGLEPYDCLS PGLMDAIATS
     VAKSQQKAA
//