Q21D57 (GFA_RHOPB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione-dependent formaldehyde-activating enzyme
EC=4.4.1.22

Alternative name(s):
S-(hydroxymethyl)glutathione synthase

Gene names

Name:	gfa
Ordered Locus Names:	RPC_0100

Organism

Rhodopseudomonas palustris (strain BisB18) [Complete proteome] [HAMAP]

Taxonomic identifier

316056 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	189 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation of formaldehyde and glutathione to S-hydroxymethylglutathione By similarity. HAMAP MF_00723
Catalytic activity	S-(hydroxymethyl)glutathione = glutathione + formaldehyde. HAMAP MF_00723
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_00723
Pathway	One-carbon metabolism; formaldehyde degradation; formate from formaldehyde (glutathione route): step 1/3. HAMAP MF_00723
Sequence similarities	Belongs to the gfa family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	formaldehyde catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	S-(hydroxymethyl)glutathione synthase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 189	189	Glutathione-dependent formaldehyde-activating enzyme HAMAP MF_00723		PRO_1000045840
Sites
	Metal binding	27	1	Zinc 1; structural By similarity
	Metal binding	29	1	Zinc 1; structural By similarity
	Metal binding	48	1	Zinc 2; catalytic By similarity
	Metal binding	50	1	Zinc 2; catalytic By similarity
	Metal binding	53	1	Zinc 2; catalytic By similarity
	Metal binding	95	1	Zinc 1; structural By similarity
	Metal binding	98	1	Zinc 1; structural By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q21D57 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: 26C883D531979A63	FASTA	189	20,028
	10 20 30 40 50 60 MTVHIHPAVD QGVKQGSGSF AGGTLVCKCQ DRPVKVGIKG DVAHNHACGC TKCWKPDGAT 70 80 90 100 110 120 FSVVAVVPRD NVTVLENGDK LHVVDASATI QRHACKVCGT HMYGRIENKS HPFYGLDFIH 130 140 150 160 170 180 PELFQEGGSA APEFAAFVSS VIESGVDPSE MPGIRARLKE LGLEPYDCLS PGLMDAIATS VAKSQQKAA

References

[1]

"Complete sequence of Rhodopseudomonas palustris BisB18." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Pelletier D.A., Kyrpides N., Anderson I., Oda Y., Harwood C.S., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BisB18.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000301 Genomic DNA. Translation: ABD85679.1.
RefSeq	YP_529998.1. NC_007925.1.
3D structure databases
ProteinModelPortal	Q21D57.
SMR	Q21D57. Positions 3-183.
ModBase	Search...
Protein-protein interaction databases
STRING	Q21D57.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3971320.
GenomeReviews	Gene locus RPC_0100 in contig CP000301_GR.
KEGG	rpc:RPC_0100.
PATRIC	23264797. VBIRhoPal29154_0114.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3791.
HOGENOM	HBG294467.
OMA	PALMDAI.
ProtClustDB	PRK05417.
Enzyme and pathway databases
BioCyc	RPAL316056:RPC_0100-MONOMER.
Family and domain databases
HAMAP	MF_00723. Formald_GSH. [Tree]
InterPro	IPR014185. Formald_GSH. IPR006913. GFA/CENP-V. IPR011057. Mss4-like. [Graphical view]
Gene3D	G3DSA:3.90.1590.10. G3DSA:3.90.1590.10. 1 hit.
KO	K03396.
Pfam	PF04828. GFA. 1 hit. [Graphical view]
PIRSF	PIRSF033318. Formald_GSH. 1 hit.
SUPFAM	SSF51316. Mss4_like. 1 hit.
TIGRFAMs	TIGR02820. Formald_GSH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GFA_RHOPB

Accession

Primary (citable) accession number: Q21D57

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 18, 2006
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program