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Q21D57 (GFA_RHOPB) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione-dependent formaldehyde-activating enzyme

EC=4.4.1.22
Alternative name(s):
S-(hydroxymethyl)glutathione synthase
Gene names
Name:gfa
Ordered Locus Names:RPC_0100
OrganismRhodopseudomonas palustris (strain BisB18) [Complete proteome] [HAMAP]
Taxonomic identifier316056 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas

Protein attributes

Sequence length189 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the condensation of formaldehyde and glutathione to S-hydroxymethylglutathione By similarity. HAMAP MF_00723

Catalytic activity

S-(hydroxymethyl)glutathione = glutathione + formaldehyde. HAMAP MF_00723

Cofactor

Binds 2 zinc ions per subunit By similarity. HAMAP MF_00723

Pathway

One-carbon metabolism; formaldehyde degradation; formate from formaldehyde (glutathione route): step 1/3. HAMAP MF_00723

Sequence similarities

Belongs to the gfa family.

Ontologies

Keywords
   LigandMetal-binding
Zinc
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processformaldehyde catabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular functionS-(hydroxymethyl)glutathione synthase activity

Inferred from electronic annotation. Source: EC

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 189189Glutathione-dependent formaldehyde-activating enzyme HAMAP MF_00723
PRO_1000045840

Sites

Metal binding271Zinc 1; structural By similarity
Metal binding291Zinc 1; structural By similarity
Metal binding481Zinc 2; catalytic By similarity
Metal binding501Zinc 2; catalytic By similarity
Metal binding531Zinc 2; catalytic By similarity
Metal binding951Zinc 1; structural By similarity
Metal binding981Zinc 1; structural By similarity

Sequences

Sequence LengthMass (Da)Tools
Q21D57 [UniParc].

Last modified April 18, 2006. Version 1.
Checksum: 26C883D531979A63

FASTA18920,028
        10         20         30         40         50         60 
MTVHIHPAVD QGVKQGSGSF AGGTLVCKCQ DRPVKVGIKG DVAHNHACGC TKCWKPDGAT 

        70         80         90        100        110        120 
FSVVAVVPRD NVTVLENGDK LHVVDASATI QRHACKVCGT HMYGRIENKS HPFYGLDFIH 

       130        140        150        160        170        180 
PELFQEGGSA APEFAAFVSS VIESGVDPSE MPGIRARLKE LGLEPYDCLS PGLMDAIATS 


VAKSQQKAA 

« Hide

References

[1]"Complete sequence of Rhodopseudomonas palustris BisB18."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Pelletier D.A., Kyrpides N., Anderson I., Oda Y., Harwood C.S., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BisB18.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000301 Genomic DNA. Translation: ABD85679.1.
RefSeqYP_529998.1. NC_007925.1.

3D structure databases

ProteinModelPortalQ21D57.
SMRQ21D57. Positions 3-183.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ21D57.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3971320.
GenomeReviewsGene locus RPC_0100 in contig CP000301_GR.
KEGGrpc:RPC_0100.
PATRIC23264797. VBIRhoPal29154_0114.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3791.
HOGENOMHBG294467.
OMAPALMDAI.
ProtClustDBPRK05417.

Enzyme and pathway databases

BioCycRPAL316056:RPC_0100-MONOMER.

Family and domain databases

HAMAPMF_00723. Formald_GSH.
[Tree]
InterProIPR014185. Formald_GSH.
IPR006913. GFA/CENP-V.
IPR011057. Mss4-like.
[Graphical view]
Gene3DG3DSA:3.90.1590.10. G3DSA:3.90.1590.10. 1 hit.
KOK03396.
PfamPF04828. GFA. 1 hit.
[Graphical view]
PIRSFPIRSF033318. Formald_GSH. 1 hit.
SUPFAMSSF51316. Mss4_like. 1 hit.
TIGRFAMsTIGR02820. Formald_GSH. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGFA_RHOPB
AccessionPrimary (citable) accession number: Q21D57
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 18, 2006
Last modified: January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families