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Reviewed, UniProtKB/Swiss-Prot Q21D57 (GFA_RHOPB)

Last modified June 16, 2009. Version 21. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Glutathione-dependent formaldehyde-activating enzyme
    EC=4.4.1.22
Alternative name(s):
    S-(hydroxymethyl)glutathione synthase
Gene names
Name: gfa
Ordered Locus Names: RPC_0100
OrganismRhodopseudomonas palustris (strain BisB18) [Complete proteome] [HAMAP]
Taxonomic identifier316056 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

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Protein attributes

Sequence length189 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Catalyzes the condensation of formaldehyde and glutathione to S-hydroxymethylglutathione By similarity.

Catalytic activity

S-(hydroxymethyl)glutathione = glutathione + formaldehyde. HAMAP MF_00723

Cofactor

Binds 2 zinc ions per subunit By similarity.

Pathway

One-carbon metabolism; formaldehyde degradation; formate from formaldehyde (glutathione route): step 1/3. HAMAP MF_00723

Sequence similarities

Belongs to the gfa family.

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Ontologies

Keywords
   LigandMetal-binding
Zinc
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processformaldehyde catabolic process

Inferred from electronic annotation. Source: HAMAP

   Molecular functionS-(hydroxymethyl)glutathione synthase activity

Inferred from electronic annotation. Source: HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 189189Glutathione-dependent formaldehyde-activating enzyme HAMAP MF_00723
PRO_1000045840

Sites

Metal binding271Zinc 1; structural By similarity
Metal binding291Zinc 1; structural By similarity
Metal binding481Zinc 2; catalytic By similarity
Metal binding501Zinc 2; catalytic By similarity
Metal binding531Zinc 2; catalytic By similarity
Metal binding951Zinc 1; structural By similarity
Metal binding981Zinc 1; structural By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q21D57-1 [UniParc].

Last modified April 18, 2006. Version 1.
Checksum: 26C883D531979A63

FASTA18920,028
        10         20         30         40         50         60 
MTVHIHPAVD QGVKQGSGSF AGGTLVCKCQ DRPVKVGIKG DVAHNHACGC TKCWKPDGAT 

        70         80         90        100        110        120 
FSVVAVVPRD NVTVLENGDK LHVVDASATI QRHACKVCGT HMYGRIENKS HPFYGLDFIH 

       130        140        150        160        170        180 
PELFQEGGSA APEFAAFVSS VIESGVDPSE MPGIRARLKE LGLEPYDCLS PGLMDAIATS 


VAKSQQKAA

« Hide

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References

[1]"Complete sequence of Rhodopseudomonas palustris BisB18."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Pelletier D.A., Kyrpides N., Anderson I., Oda Y., Harwood C.S., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

CP000301 Genomic DNA. Translation: ABD85679.1.
RefSeqYP_529998.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID3971320.
GenomeReviewsGene locus RPC_0100 in contig CP000301_GR.
KEGGrpc:RPC_0100.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ21D57.
OMAQ21D57. HACGCTK.

Enzyme and pathway databases

BioCycRPAL316056:RPC_0100-MON.

Family and domain databases

HAMAPMF_00723.
[Tree]
InterProIPR014185. Formald_GSH.
IPR006913. GFA.
[Graphical view]
PfamPF04828. GFA. 1 hit.
[Graphical view]
PIRSFPIRSF033318. Formald_GSH. 1 hit.
TIGRFAMsTIGR02820. formald_GSH. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameGFA_RHOPB
AccessionPrimary (citable) accession number: Q21D57
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 18, 2006
Last modified: June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents