Reviewed, UniProtKB/Swiss-Prot Q21CW7 (MDH1_RHOPB)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase 1
    EC=1.1.1.37

Gene names

Name:	mdh1
Ordered Locus Names:	RPC_0194

Organism

Rhodopseudomonas palustris (strain BisB18) [Complete proteome] [HAMAP]

Taxonomic identifier

316056 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP MF_00487
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	Malate dehydrogenase 1 HAMAP MF_00487		PRO_0000241962
Regions
	Nucleotide binding	10 – 15	6	NAD By similarity
	Nucleotide binding	119 – 121	3	NAD By similarity
Sites
	Active site	176	1	Proton acceptor By similarity
	Binding site	34	1	NAD By similarity
	Binding site	83	1	Substrate By similarity
	Binding site	89	1	Substrate By similarity
	Binding site	96	1	NAD By similarity
	Binding site	121	1	Substrate By similarity
	Binding site	152	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q21CW7-1 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: 4C73F78BA98959CB	FASTA	322	34,161
	10 20 30 40 50 60 MARDKIALIG SGQIGGTLAH LVGLKELGDV VMFDIAEGIP QGKSLDIAQS SPVDGFDAKF 70 80 90 100 110 120 TGANSYEAIE GARVVIVTAG VPRKPGMSRD DLLSINLKVM EQVGAGIKKY APDAFVICIT 130 140 150 160 170 180 NPLDAMVWAL QKASGLPHRK VVGMAGVLDS ARFRYFLADE FNVSVEDVTA FVLGGHGDTM 190 200 210 220 230 240 VPLVKYSTVA GIPLPDLVKM GWTSQARLDE IVDRTRNGGA EIVNLLKTGS AFYAPAASAI 250 260 270 280 290 300 AMAESYLKDK KRVVPVAAYL NGEYGVKDMY VGVPVVIGDK GVERIVEIEL AGKDREAFDK 310 320 SVAAVQGLVE ACKKIAPELL GH

References

[1]

"Complete sequence of Rhodopseudomonas palustris BisB18." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Pelletier D.A., Kyrpides N., Anderson I., Oda Y., Harwood C.S., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000301 Genomic DNA. Translation: ABD85769.1.
RefSeq	YP_530088.1.
3D structure databases
SMR	Q21CW7. Positions 1-313.
ModBase	Search...
Protein-protein interaction databases
STRING	Q21CW7.
Genome annotation databases
GeneID	3971182.
GenomeReviews	Gene locus RPC_0194 in contig CP000301_GR.
KEGG	rpc:RPC_0194.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HBG566126.
OMA	YFLADEF.
Enzyme and pathway databases
BioCyc	RPAL316056:RPC_0194-MONOMER.
Family and domain databases
HAMAP	MF_00487. Malate_dehydrog_3. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR011275. Malate_DH_NAD-dep_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01763. MalateDH_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH1_RHOPB

Accession

Primary (citable) accession number: Q21CW7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	April 18, 2006
Last modified:	February 9, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)