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Q21AR4

- NAPA_RHOPB

UniProt

Q21AR4 - NAPA_RHOPB

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Rhodopseudomonas palustris (strain BisB18)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (18 Apr 2006)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi54 – 541Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi58 – 581Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi86 – 861Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciRPAL316056:GH3E-964-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:RPC_0952
    OrganismiRhodopseudomonas palustris (strain BisB18)
    Taxonomic identifieri316056 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
    ProteomesiUP000001948: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3333Tat-type signalUniRule annotationAdd
    BLAST
    Chaini34 – 837804Periplasmic nitrate reductasePRO_0000256075Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi316056.RPC_0952.

    Structurei

    3D structure databases

    ProteinModelPortaliQ21AR4.
    SMRiQ21AR4. Positions 44-832.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini44 – 100574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiFNQHQRG.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q21AR4-1 [UniParc]FASTAAdd to Basket

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    MTTPKLDRRQ VLKLEAAAMA ALAGGIAMPA AAANLVTERA VSELKWDKAA    50
    CRFCGTGCSV MVATKENRVV ATHGDTKSEV NRGLNCVKGY FLSKIMYGHD 100
    RLTQPMLRKT DGKYDKNGEF MPVSWDEAFD IMAEKFKAAL KKRGPSGVGM 150
    FGSGQWTVWE GYAASKLFKA GFRSNNIDPN ARHCMASAVA GFMRTFGIDE 200
    PMGCYDDIEA ADVFVLWGSN MAEMHPLLWT RVTDRRLSAP HVKVAVLSTF 250
    EHRSFDLADL GLVFKPQTDL AILNAIANHI IKTGRVNKDF VAKHTTFKRG 300
    QTDIGYGLRP EHPLQKAATG AAKANDATDM SFEDYAAFVA DYTIEKASQI 350
    SGVPAAKIEA LAELYADPNT KVTSFWTMGF NQHTRGVWAN NLAYNLHLLT 400
    GKISEPGNSP FSLTGQPSAC GTAREVGTFS HRLPADMVVT NKKHRDIAEK 450
    IWKLPEGTIP DKPGYHAVLQ SRMLKDGLLN AYWVQVNNNL QAGANANEET 500
    YPGFRNPDNF IVVSDAYPSV TALAADLILP TAMWVEKEGA YGNAERRTQF 550
    WHQLVSAPGQ ARSDLWQLME FAKRFKIEEV WTEELLAKKP EVRGKTMYDV 600
    LYRNGQVDKY PSSDIEAGYL NDESKAFGYY VQKGLFEEYA SFGRGHGHDL 650
    APFDDYHRER GLRWPVVNGQ ETRWRFREGS DPYVQQGAGV QFYGFPDGRA 700
    RIFALPYEPA AEAPDAEFPF WLSTGRVLEH WHSGTMTRRV PELYKAFPEA 750
    VCFMHPDDAA ELKLRRGDEI KVESRRGFIR TRVETRGRNK PPRGLVFVPW 800
    FDEAQLINKV TLDATDPISL QTDYKKCAVR IERVTAS 837
    Length:837
    Mass (Da):93,633
    Last modified:April 18, 2006 - v1
    Checksum:iCA1EB7B6C1D9B06F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000301 Genomic DNA. Translation: ABD86522.1.
    RefSeqiYP_530841.1. NC_007925.1.

    Genome annotation databases

    EnsemblBacteriaiABD86522; ABD86522; RPC_0952.
    GeneIDi3969715.
    KEGGirpc:RPC_0952.
    PATRICi23266562. VBIRhoPal29154_0987.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000301 Genomic DNA. Translation: ABD86522.1 .
    RefSeqi YP_530841.1. NC_007925.1.

    3D structure databases

    ProteinModelPortali Q21AR4.
    SMRi Q21AR4. Positions 44-832.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 316056.RPC_0952.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABD86522 ; ABD86522 ; RPC_0952 .
    GeneIDi 3969715.
    KEGGi rpc:RPC_0952.
    PATRICi 23266562. VBIRhoPal29154_0987.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi FNQHQRG.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci RPAL316056:GH3E-964-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: BisB18.

    Entry informationi

    Entry nameiNAPA_RHOPB
    AccessioniPrimary (citable) accession number: Q21AR4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2006
    Last sequence update: April 18, 2006
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3