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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Rhodopseudomonas palustris (strain BisB18)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi51Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi54Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi58Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi86Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei88Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei155Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei180Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei184Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei378Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei382Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei488Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei537Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei564Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei800Substrate; via amide nitrogenUniRule annotation1
Binding sitei808Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei825Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciRPAL316056:G1G6B-964-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:RPC_0952
OrganismiRhodopseudomonas palustris (strain BisB18)
Taxonomic identifieri316056 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
Proteomesi
  • UP000001948 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 33Tat-type signalUniRule annotationAdd BLAST33
ChainiPRO_000025607534 – 837Periplasmic nitrate reductaseUniRule annotationAdd BLAST804

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Protein-protein interaction databases

STRINGi316056.RPC_0952

Structurei

3D structure databases

ProteinModelPortaliQ21AR4
SMRiQ21AR4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 1004Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni217 – 224Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni248 – 252Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation5
Regioni267 – 269Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation3
Regioni514 – 515Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni724 – 733Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW Bacteria
COG0243 LUCA
HOGENOMiHOG000031441
KOiK02567
OMAiANSMGTR
OrthoDBiPOG091H060P

Family and domain databases

HAMAPiMF_01630 Nitrate_reduct_NapA, 1 hit
InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR027467 MopterinOxRdtase_cofactor_BS
IPR010051 Periplasm_NO3_reductase_lsu
IPR006311 TAT_signal
PANTHERiPTHR11615:SF123 PTHR11615:SF123, 1 hit
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR01706 NAPA, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q21AR4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTPKLDRRQ VLKLEAAAMA ALAGGIAMPA AAANLVTERA VSELKWDKAA
60 70 80 90 100
CRFCGTGCSV MVATKENRVV ATHGDTKSEV NRGLNCVKGY FLSKIMYGHD
110 120 130 140 150
RLTQPMLRKT DGKYDKNGEF MPVSWDEAFD IMAEKFKAAL KKRGPSGVGM
160 170 180 190 200
FGSGQWTVWE GYAASKLFKA GFRSNNIDPN ARHCMASAVA GFMRTFGIDE
210 220 230 240 250
PMGCYDDIEA ADVFVLWGSN MAEMHPLLWT RVTDRRLSAP HVKVAVLSTF
260 270 280 290 300
EHRSFDLADL GLVFKPQTDL AILNAIANHI IKTGRVNKDF VAKHTTFKRG
310 320 330 340 350
QTDIGYGLRP EHPLQKAATG AAKANDATDM SFEDYAAFVA DYTIEKASQI
360 370 380 390 400
SGVPAAKIEA LAELYADPNT KVTSFWTMGF NQHTRGVWAN NLAYNLHLLT
410 420 430 440 450
GKISEPGNSP FSLTGQPSAC GTAREVGTFS HRLPADMVVT NKKHRDIAEK
460 470 480 490 500
IWKLPEGTIP DKPGYHAVLQ SRMLKDGLLN AYWVQVNNNL QAGANANEET
510 520 530 540 550
YPGFRNPDNF IVVSDAYPSV TALAADLILP TAMWVEKEGA YGNAERRTQF
560 570 580 590 600
WHQLVSAPGQ ARSDLWQLME FAKRFKIEEV WTEELLAKKP EVRGKTMYDV
610 620 630 640 650
LYRNGQVDKY PSSDIEAGYL NDESKAFGYY VQKGLFEEYA SFGRGHGHDL
660 670 680 690 700
APFDDYHRER GLRWPVVNGQ ETRWRFREGS DPYVQQGAGV QFYGFPDGRA
710 720 730 740 750
RIFALPYEPA AEAPDAEFPF WLSTGRVLEH WHSGTMTRRV PELYKAFPEA
760 770 780 790 800
VCFMHPDDAA ELKLRRGDEI KVESRRGFIR TRVETRGRNK PPRGLVFVPW
810 820 830
FDEAQLINKV TLDATDPISL QTDYKKCAVR IERVTAS
Length:837
Mass (Da):93,633
Last modified:April 18, 2006 - v1
Checksum:iCA1EB7B6C1D9B06F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000301 Genomic DNA Translation: ABD86522.1
RefSeqiWP_011471429.1, NC_007925.1

Genome annotation databases

EnsemblBacteriaiABD86522; ABD86522; RPC_0952
KEGGirpc:RPC_0952

Similar proteinsi

Entry informationi

Entry nameiNAPA_RHOPB
AccessioniPrimary (citable) accession number: Q21AR4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: April 18, 2006
Last modified: March 28, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health