Reviewed, UniProtKB/Swiss-Prot Q21AR4 (NAPA_RHOPB)

Names and origin

Protein names

Recommended name:
    Periplasmic nitrate reductase
    EC=1.7.99.4

Gene names

Name:	napA
Ordered Locus Names:	RPC_0952

Organism

Rhodopseudomonas palustris (strain BisB18) [Complete proteome] [HAMAP]

Taxonomic identifier

316056 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	837 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP MF_01630
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor. HAMAP MF_01630
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP MF_01630 Binds 1 molybdenum ion per subunit By similarity. HAMAP MF_01630 Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity. HAMAP MF_01630
Subunit structure	Interacts with napB By similarity. HAMAP MF_01630
Subcellular location	Periplasm By similarity HAMAP MF_01630.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP MF_01630
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 33	33	Tat-type signal Potential
	Chain	34 – 837	804	Periplasmic nitrate reductase HAMAP MF_01630		PRO_0000256075
Sites
	Metal binding	51	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	54	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	58	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	86	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q21AR4-1 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: CA1EB7B6C1D9B06F	FASTA	837	93,633
	10 20 30 40 50 60 MTTPKLDRRQ VLKLEAAAMA ALAGGIAMPA AAANLVTERA VSELKWDKAA CRFCGTGCSV 70 80 90 100 110 120 MVATKENRVV ATHGDTKSEV NRGLNCVKGY FLSKIMYGHD RLTQPMLRKT DGKYDKNGEF 130 140 150 160 170 180 MPVSWDEAFD IMAEKFKAAL KKRGPSGVGM FGSGQWTVWE GYAASKLFKA GFRSNNIDPN 190 200 210 220 230 240 ARHCMASAVA GFMRTFGIDE PMGCYDDIEA ADVFVLWGSN MAEMHPLLWT RVTDRRLSAP 250 260 270 280 290 300 HVKVAVLSTF EHRSFDLADL GLVFKPQTDL AILNAIANHI IKTGRVNKDF VAKHTTFKRG 310 320 330 340 350 360 QTDIGYGLRP EHPLQKAATG AAKANDATDM SFEDYAAFVA DYTIEKASQI SGVPAAKIEA 370 380 390 400 410 420 LAELYADPNT KVTSFWTMGF NQHTRGVWAN NLAYNLHLLT GKISEPGNSP FSLTGQPSAC 430 440 450 460 470 480 GTAREVGTFS HRLPADMVVT NKKHRDIAEK IWKLPEGTIP DKPGYHAVLQ SRMLKDGLLN 490 500 510 520 530 540 AYWVQVNNNL QAGANANEET YPGFRNPDNF IVVSDAYPSV TALAADLILP TAMWVEKEGA 550 560 570 580 590 600 YGNAERRTQF WHQLVSAPGQ ARSDLWQLME FAKRFKIEEV WTEELLAKKP EVRGKTMYDV 610 620 630 640 650 660 LYRNGQVDKY PSSDIEAGYL NDESKAFGYY VQKGLFEEYA SFGRGHGHDL APFDDYHRER 670 680 690 700 710 720 GLRWPVVNGQ ETRWRFREGS DPYVQQGAGV QFYGFPDGRA RIFALPYEPA AEAPDAEFPF 730 740 750 760 770 780 WLSTGRVLEH WHSGTMTRRV PELYKAFPEA VCFMHPDDAA ELKLRRGDEI KVESRRGFIR 790 800 810 820 830 TRVETRGRNK PPRGLVFVPW FDEAQLINKV TLDATDPISL QTDYKKCAVR IERVTAS

References

[1]

"Complete sequence of Rhodopseudomonas palustris BisB18." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Pelletier D.A., Kyrpides N., Anderson I., Oda Y., Harwood C.S., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000301 Genomic DNA. Translation: ABD86522.1.
RefSeq	YP_530841.1.
3D structure databases
SMR	Q21AR4. Positions 44-832.
ModBase	Search...
Protein-protein interaction databases
STRING	Q21AR4.
Genome annotation databases
GeneID	3969715.
GenomeReviews	Gene locus RPC_0952 in contig CP000301_GR.
KEGG	rpc:RPC_0952.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0243.
HOGENOM	HBG715033.
OMA	QFWHQLV.
Enzyme and pathway databases
BioCyc	RPAL316056:RPC_0952-MONOMER.
Family and domain databases
HAMAP	MF_01630. Nitrate_reduct. [Tree]
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4_dom. IPR006655. Mopterin_OxRdtase_prok_CS. IPR010051. NO3_reductase_lsu_periplasm. IPR006311. TAT_signal. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA_RHOPB

Accession

Primary (citable) accession number: Q21AR4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	April 18, 2006
Last modified:	February 9, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)