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Q21AR4 (NAPA_RHOPB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:RPC_0952
OrganismRhodopseudomonas palustris (strain BisB18) [Complete proteome] [HAMAP]
Taxonomic identifier316056 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas

Protein attributes

Sequence length837 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3333Tat-type signal Potential
Chain34 – 837804Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_0000256075

Regions

Domain44 – 100574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding511Iron-sulfur (4Fe-4S) By similarity
Metal binding541Iron-sulfur (4Fe-4S) By similarity
Metal binding581Iron-sulfur (4Fe-4S) By similarity
Metal binding861Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q21AR4 [UniParc].

Last modified April 18, 2006. Version 1.
Checksum: CA1EB7B6C1D9B06F

FASTA83793,633
        10         20         30         40         50         60 
MTTPKLDRRQ VLKLEAAAMA ALAGGIAMPA AAANLVTERA VSELKWDKAA CRFCGTGCSV 

        70         80         90        100        110        120 
MVATKENRVV ATHGDTKSEV NRGLNCVKGY FLSKIMYGHD RLTQPMLRKT DGKYDKNGEF 

       130        140        150        160        170        180 
MPVSWDEAFD IMAEKFKAAL KKRGPSGVGM FGSGQWTVWE GYAASKLFKA GFRSNNIDPN 

       190        200        210        220        230        240 
ARHCMASAVA GFMRTFGIDE PMGCYDDIEA ADVFVLWGSN MAEMHPLLWT RVTDRRLSAP 

       250        260        270        280        290        300 
HVKVAVLSTF EHRSFDLADL GLVFKPQTDL AILNAIANHI IKTGRVNKDF VAKHTTFKRG 

       310        320        330        340        350        360 
QTDIGYGLRP EHPLQKAATG AAKANDATDM SFEDYAAFVA DYTIEKASQI SGVPAAKIEA 

       370        380        390        400        410        420 
LAELYADPNT KVTSFWTMGF NQHTRGVWAN NLAYNLHLLT GKISEPGNSP FSLTGQPSAC 

       430        440        450        460        470        480 
GTAREVGTFS HRLPADMVVT NKKHRDIAEK IWKLPEGTIP DKPGYHAVLQ SRMLKDGLLN 

       490        500        510        520        530        540 
AYWVQVNNNL QAGANANEET YPGFRNPDNF IVVSDAYPSV TALAADLILP TAMWVEKEGA 

       550        560        570        580        590        600 
YGNAERRTQF WHQLVSAPGQ ARSDLWQLME FAKRFKIEEV WTEELLAKKP EVRGKTMYDV 

       610        620        630        640        650        660 
LYRNGQVDKY PSSDIEAGYL NDESKAFGYY VQKGLFEEYA SFGRGHGHDL APFDDYHRER 

       670        680        690        700        710        720 
GLRWPVVNGQ ETRWRFREGS DPYVQQGAGV QFYGFPDGRA RIFALPYEPA AEAPDAEFPF 

       730        740        750        760        770        780 
WLSTGRVLEH WHSGTMTRRV PELYKAFPEA VCFMHPDDAA ELKLRRGDEI KVESRRGFIR 

       790        800        810        820        830 
TRVETRGRNK PPRGLVFVPW FDEAQLINKV TLDATDPISL QTDYKKCAVR IERVTAS 

« Hide

References

[1]"Complete sequence of Rhodopseudomonas palustris BisB18."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Pelletier D.A., Kyrpides N., Anderson I., Oda Y., Harwood C.S., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BisB18.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000301 Genomic DNA. Translation: ABD86522.1.
RefSeqYP_530841.1. NC_007925.1.

3D structure databases

ProteinModelPortalQ21AR4.
SMRQ21AR4. Positions 44-832.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING316056.RPC_0952.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD86522; ABD86522; RPC_0952.
GeneID3969715.
KEGGrpc:RPC_0952.
PATRIC23266562. VBIRhoPal29154_0987.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAFNQHQRG.
OrthoDBEOG6CVV7G.

Enzyme and pathway databases

BioCycRPAL316056:GH3E-964-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_RHOPB
AccessionPrimary (citable) accession number: Q21AR4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: April 18, 2006
Last modified: May 14, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families