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Protein

Mediator of RNA polymerase II transcription subunit 15

Gene

mdt-15

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity). Required for regulated expression of genes controlling fatty acid desaturation by factors including sbp-1 and nhr-49.By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

  • determination of adult lifespan Source: WormBase
  • embryo development ending in birth or egg hatching Source: WormBase
  • locomotion Source: WormBase
  • mRNA splicing, via spliceosome Source: GO_Central
  • nematode larval development Source: WormBase
  • positive regulation of transcription, DNA-templated Source: WormBase
  • regulation of fatty acid metabolic process Source: WormBase
  • regulation of gene expression Source: WormBase
  • regulation of multicellular organism growth Source: WormBase
  • sequestering of triglyceride Source: WormBase
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 15
Alternative name(s):
Mediator complex subunit 15
Gene namesi
Name:mdt-15
ORF Names:R12B2.5
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiR12B2.5a; CE30108; WBGene00007016; mdt-15.
R12B2.5b; CE25078; WBGene00007016; mdt-15.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000654341 – 780Mediator of RNA polymerase II transcription subunit 15Add BLAST780

Proteomic databases

EPDiQ21955.
PaxDbiQ21955.
PeptideAtlasiQ21955.
PRIDEiQ21955.

Expressioni

Tissue specificityi

Expressed in the intestine and head neurons.1 Publication

Developmental stagei

Expressed throughout development.1 Publication

Gene expression databases

BgeeiWBGene00007016.

Interactioni

Subunit structurei

Component of the Mediator complex (By similarity). Interacts with nhr-49, nhr-64 and sbp-1.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
nhr-49O456664EBI-318240,EBI-318820

GO - Molecular functioni

  • nuclear hormone receptor binding Source: WormBase
  • protein dimerization activity Source: InterPro

Protein-protein interaction databases

BioGridi41039. 5 interactors.
DIPiDIP-38923N.
IntActiQ21955. 14 interactors.
MINTiMINT-1092073.
STRINGi6239.R12B2.5a.1.

Structurei

3D structure databases

ProteinModelPortaliQ21955.
SMRiQ21955.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 124Interaction with nhr-49Add BLAST124
Regioni2 – 96Interaction with sbp-1Add BLAST95

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi175 – 247Gly-richAdd BLAST73
Compositional biasi252 – 352Gln-richAdd BLAST101
Compositional biasi441 – 609Met-richAdd BLAST169

Sequence similaritiesi

Belongs to the Mediator complex subunit 15 family.Curated

Phylogenomic databases

eggNOGiKOG4274. Eukaryota.
ENOG41112VF. LUCA.
HOGENOMiHOG000113521.
InParanoidiQ21955.
OMAiFREHVIQ.
OrthoDBiEOG091G06TE.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiView protein in InterPro
IPR036638. HLH_DNA-bd_sf.
IPR019087. Med15.
PfamiView protein in Pfam
PF09606. Med15. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q21955-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEEDWPSPK FREHVIQRLE PELARNRQNA PNLPVPGDAR QVEEYVFAKC
60 70 80 90 100
MSKDEYMRTI AKVINAINCN SKSAAVPSVL QPSQFHSPPC TTAALLGNTP
110 120 130 140 150
AGGTPGYRAP VPPDPQPTSA QARNPPVTVA TTQASTTPSA PNPPGGLPAP
160 170 180 190 200
SASATAAVAA AVASFPSPDT SIRPGGQITP GSQAPGGGPT PAPNVPFPNG
210 220 230 240 250
SSQMNGGPAM GQPPPQMGAP NMGGPPNGYG GYGMMNGPPG SGAPMGGNPY
260 270 280 290 300
NQQIKKDMDQ ARPWDPSAHM YQQQPQWGAM PPQQPHGYPG RPMNGQGATP
310 320 330 340 350
TGPSSVLESL INQPQQYPGH HNQMGPPGDR NVAAQRAAAQ QQQQQQQQQQ
360 370 380 390 400
QQRPGMVPNQ GMMSSEDQTV YSAKLRNMRG SCDSLRTRAR QCRHEGNHEA
410 420 430 440 450
AHKLEVMLSV LEGKRVVSLE YLNHLEMWIA RKQDFLNIAP MSQNQNHMGM
460 470 480 490 500
NDPMMNGEHA MLGNGQVPNP YGGHPGYGHQ QYMGPPPPHM QMHQPPMWHQ
510 520 530 540 550
QQHQQQQRMM PQDHMMMQGG GGPVHGMYRG DMGHDPMTSP VNNHRHAPYP
560 570 580 590 600
NPAAMRNNMR MPNGPGPIGR DRNSMSGSSM SGPSSGAPSM NPMGTPNQKM
610 620 630 640 650
GTPGSMGGMS GLDDLNYDDF LPNPTPIDAL QPTLHVGQNS MNAGPPVQRS
660 670 680 690 700
NLNETARKEL QILDARFEID PNHQRHDANH IIVVCKIRNQ QFPPLRLVVP
710 720 730 740 750
TTYPAGNVTV DRAVIDLDAY LYDDLQNSVY ERLSRPGLSS ITDYLNAWEE
760 770 780
QVNQYQNQTS GGLDVAFNVG NDFFYDNLNL
Note: No experimental confirmation available.
Length:780
Mass (Da):84,528
Last modified:March 1, 2002 - v3
Checksum:i4259A33584603D0A
GO
Isoform b (identifier: Q21955-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     94-96: Missing.

Note: No experimental confirmation available.
Show »
Length:777
Mass (Da):84,230
Checksum:i489F9D46BA7F0BFC
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02062494 – 96Missing in isoform b. Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081686 Genomic DNA. Translation: CCD73316.1.
FO081686 Genomic DNA. Translation: CCD73317.1.
PIRiT16726.
RefSeqiNP_741151.1. NM_171134.6. [Q21955-2]
NP_741152.1. NM_171867.4. [Q21955-1]
UniGeneiCel.38778.

Genome annotation databases

EnsemblMetazoaiR12B2.5a.1; R12B2.5a.1; WBGene00007016. [Q21955-1]
R12B2.5a.2; R12B2.5a.2; WBGene00007016. [Q21955-1]
R12B2.5a.3; R12B2.5a.3; WBGene00007016. [Q21955-1]
GeneIDi175817.
KEGGicel:CELE_R12B2.5.
UCSCiR12B2.5b.2. c. elegans. [Q21955-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMED15_CAEEL
AccessioniPrimary (citable) accession number: Q21955
Secondary accession number(s): Q8MPZ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: March 1, 2002
Last modified: October 25, 2017
This is version 115 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families