Q21926 (SYIC_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isoleucine--tRNA ligase, cytoplasmic
EC=6.1.1.5

Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS

Gene names

Name:	irs-1
ORF Names:	R11A8.6

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	1141 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1141	1141	Isoleucine--tRNA ligase, cytoplasmic		PRO_0000098599
Regions
	Motif	50 – 60	11	"HIGH" region
	Motif	601 – 605	5	"KMSKS" region
Sites
	Binding site	604	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q21926 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 151E6AD6D6360EB0	FASTA	1,141	130,012
	10 20 30 40 50 60 MSGLSTVPDN INFAREEDKV AQKWQDENTF QRSVELSKDR PHFTFYDGPP FATGLPHYGH 70 80 90 100 110 120 MLTSTIKDVV GRWAHQNGHY VERRFGWDTH GLPVEYEIDK TLGISGPQDV MKMGIANYNN 130 140 150 160 170 180 ECRKIVMRYS GEWEKTMGRL GRWVDFKHDY KTLYPWFMES VWWAFSELHK KGLVYKGVKV 190 200 210 220 230 240 MPFSTACSTP LSNFEAGQNY KDVVDPAVFV GFKLLDCPNR QLVAWTTTPW TLPSNLALVV 250 260 270 280 290 300 HPDMLYVVTK DKTTGIEYVV LEERLGELKN DNLEVIEKLA GSQLKDLRYE PLFPYFAYMR 310 320 330 340 350 360 EERNAFRVLN DTFVTSDSGT GVVHQAPYFG EIDFQVCVAN GVIAKDQKMI CPVDESGKYT 370 380 390 400 410 420 SEVPDYQGVY VKDADKLIIK RLKEMGNLVR QAEVKHSYPF CWRSDTPLLY KAVPSWFINV 430 440 450 460 470 480 ETLIPRLLAN NDETYWVPAF VKEKRFANWL RDARDWAVSR NRFWGTPINL WVSEDGEEVV 490 500 510 520 530 540 CVGSIAELEE LSGQKITDLH RESVDDVTIP SRSGRGVLKR VSEVFDCWFE SGSMPYAQNH 550 560 570 580 590 600 YPFENRKIFE DNFPADFIAE GIDQTRGWFY TLLVLSTALF NKPPFKNLIC NGLVLASDGA 610 620 630 640 650 660 KMSKSKKNYP DPMLIVNKYG ADALRLYLIN SPVVRGENLR FREEGVRDLL KDVFLPWFNA 670 680 690 700 710 720 YRFFVQNVQA YEHETGNVFD MNVHVASENV MDRWIESFTN SLVAFVRKEM DSYRLYAVVG 730 740 750 760 770 780 PLTKFFDTLT NIYIRLNRKR VKGDNGLHEQ HHALAALGRV LILIVRLMAP FTPFFCEYIW 790 800 810 820 830 840 LNLKKVIGST EESVHFLMLP KPDESLIDET VERRVEVMRN VIDLVRLVRD REGLAVKYPL 850 860 870 880 890 900 KEMIVINRDS QFLEDVKSLE SYILLELNVR KLTVSQDKQK YGITLKAEPN FKILGARLKG 910 920 930 940 950 960 EQKKVADYLK NQITESELEK FLLEGKLTVL GHELSSEEVA VSYAAGSDQG HGYKTHSDAK 970 980 990 1000 1010 1020 TIVMIDTTED ESLIEEGLCR EVTNRVQRLR KQAKLVSTDT AHVHIVVSPA DSQLAKVVAA 1030 1040 1050 1060 1070 1080 KLNDIVSATG TPIKLGSPPA AAKAPTATSK SAIKDSEVEL WLFAEGDSFD GITVVDGSKK 1090 1100 1110 1120 1130 1140 VRVHVKTEKE HLKGYADLLY HVRSALDLWN GKIALKNVDG SAVHPTVDVT SLVGQTLQLV R

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z70310 Genomic DNA. Translation: CAA94369.1.
PIR	T24176.
RefSeq	NP_501914.1. NM_069513.4.
3D structure databases
ProteinModelPortal	Q21926.
SMR	Q21926. Positions 12-834.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q21926. 1 interaction.
MINT	MINT-114690.
STRING	6239.R11A8.6.
Proteomic databases
PaxDb	Q21926.
PRIDE	Q21926.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	R11A8.6.1; R11A8.6.1; R11A8.6. R11A8.6.2; R11A8.6.2; R11A8.6.
GeneID	177926.
KEGG	cel:CELE_R11A8.6.
UCSC	R11A8.6. c. elegans.
Organism-specific databases
CTD	177926.
WormBase	R11A8.6; CE06304; WBGene00002152; iars-1.
Phylogenomic databases
eggNOG	COG0060.
GeneTree	ENSGT00550000074921.
HOGENOM	HOG000246403.
InParanoid	Q21926.
KO	K01870.
OMA	SRSRYWG.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 1 hit.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-ligase. IPR023586. Ile-tRNA-ligase_type2. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	898988.

Entry information

Entry name

SYIC_CAEEL

Accession

Primary (citable) accession number: Q21926

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project