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Q21926 (SYIC_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase, cytoplasmic

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:irs-1
ORF Names:R11A8.6
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length1141 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02003

Subcellular location

Cytoplasm HAMAP-Rule MF_02003.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processisoleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

isoleucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11411141Isoleucine--tRNA ligase, cytoplasmic HAMAP-Rule MF_02003
PRO_0000098599

Regions

Motif50 – 6011"HIGH" region HAMAP-Rule MF_02003
Motif601 – 6055"KMSKS" region HAMAP-Rule MF_02003

Sites

Binding site6041ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q21926 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 151E6AD6D6360EB0

FASTA1,141130,012
        10         20         30         40         50         60 
MSGLSTVPDN INFAREEDKV AQKWQDENTF QRSVELSKDR PHFTFYDGPP FATGLPHYGH 

        70         80         90        100        110        120 
MLTSTIKDVV GRWAHQNGHY VERRFGWDTH GLPVEYEIDK TLGISGPQDV MKMGIANYNN 

       130        140        150        160        170        180 
ECRKIVMRYS GEWEKTMGRL GRWVDFKHDY KTLYPWFMES VWWAFSELHK KGLVYKGVKV 

       190        200        210        220        230        240 
MPFSTACSTP LSNFEAGQNY KDVVDPAVFV GFKLLDCPNR QLVAWTTTPW TLPSNLALVV 

       250        260        270        280        290        300 
HPDMLYVVTK DKTTGIEYVV LEERLGELKN DNLEVIEKLA GSQLKDLRYE PLFPYFAYMR 

       310        320        330        340        350        360 
EERNAFRVLN DTFVTSDSGT GVVHQAPYFG EIDFQVCVAN GVIAKDQKMI CPVDESGKYT 

       370        380        390        400        410        420 
SEVPDYQGVY VKDADKLIIK RLKEMGNLVR QAEVKHSYPF CWRSDTPLLY KAVPSWFINV 

       430        440        450        460        470        480 
ETLIPRLLAN NDETYWVPAF VKEKRFANWL RDARDWAVSR NRFWGTPINL WVSEDGEEVV 

       490        500        510        520        530        540 
CVGSIAELEE LSGQKITDLH RESVDDVTIP SRSGRGVLKR VSEVFDCWFE SGSMPYAQNH 

       550        560        570        580        590        600 
YPFENRKIFE DNFPADFIAE GIDQTRGWFY TLLVLSTALF NKPPFKNLIC NGLVLASDGA 

       610        620        630        640        650        660 
KMSKSKKNYP DPMLIVNKYG ADALRLYLIN SPVVRGENLR FREEGVRDLL KDVFLPWFNA 

       670        680        690        700        710        720 
YRFFVQNVQA YEHETGNVFD MNVHVASENV MDRWIESFTN SLVAFVRKEM DSYRLYAVVG 

       730        740        750        760        770        780 
PLTKFFDTLT NIYIRLNRKR VKGDNGLHEQ HHALAALGRV LILIVRLMAP FTPFFCEYIW 

       790        800        810        820        830        840 
LNLKKVIGST EESVHFLMLP KPDESLIDET VERRVEVMRN VIDLVRLVRD REGLAVKYPL 

       850        860        870        880        890        900 
KEMIVINRDS QFLEDVKSLE SYILLELNVR KLTVSQDKQK YGITLKAEPN FKILGARLKG 

       910        920        930        940        950        960 
EQKKVADYLK NQITESELEK FLLEGKLTVL GHELSSEEVA VSYAAGSDQG HGYKTHSDAK 

       970        980        990       1000       1010       1020 
TIVMIDTTED ESLIEEGLCR EVTNRVQRLR KQAKLVSTDT AHVHIVVSPA DSQLAKVVAA 

      1030       1040       1050       1060       1070       1080 
KLNDIVSATG TPIKLGSPPA AAKAPTATSK SAIKDSEVEL WLFAEGDSFD GITVVDGSKK 

      1090       1100       1110       1120       1130       1140 
VRVHVKTEKE HLKGYADLLY HVRSALDLWN GKIALKNVDG SAVHPTVDVT SLVGQTLQLV 


R 

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References

[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z70310 Genomic DNA. Translation: CAA94369.1.
PIRT24176.
RefSeqNP_501914.1. NM_069513.4.
UniGeneCel.17394.

3D structure databases

ProteinModelPortalQ21926.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid43028. 1 interaction.
IntActQ21926. 1 interaction.
MINTMINT-114690.
STRING6239.R11A8.6.

Proteomic databases

PaxDbQ21926.
PRIDEQ21926.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaR11A8.6.1; R11A8.6.1; WBGene00002152.
R11A8.6.2; R11A8.6.2; WBGene00002152.
GeneID177926.
KEGGcel:CELE_R11A8.6.
UCSCR11A8.6. c. elegans.

Organism-specific databases

CTD177926.
WormBaseR11A8.6; CE06304; WBGene00002152; iars-1.

Phylogenomic databases

eggNOGCOG0060.
GeneTreeENSGT00550000074921.
HOGENOMHOG000246403.
InParanoidQ21926.
KOK01870.
OMARVEHMVE.
PhylomeDBQ21926.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02003. Ile_tRNA_synth_type2.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio898988.
PROQ21926.

Entry information

Entry nameSYIC_CAEEL
AccessionPrimary (citable) accession number: Q21926
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries