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Protein

Isoleucine--tRNA ligase, cytoplasmic

Gene

irs-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei604 – 6041ATPBy similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. isoleucine-tRNA ligase activity Source: GO_Central

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase, cytoplasmic (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:irs-1
ORF Names:R11A8.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome IV

Organism-specific databases

WormBaseiR11A8.6; CE06304; WBGene00002152; iars-1.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11411141Isoleucine--tRNA ligase, cytoplasmicPRO_0000098599Add
BLAST

Proteomic databases

PaxDbiQ21926.
PRIDEiQ21926.

Interactioni

Protein-protein interaction databases

BioGridi43028. 1 interaction.
IntActiQ21926. 1 interaction.
MINTiMINT-114690.
STRINGi6239.R11A8.6.

Structurei

3D structure databases

ProteinModelPortaliQ21926.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi50 – 6011"HIGH" regionAdd
BLAST
Motifi601 – 6055"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
GeneTreeiENSGT00550000074921.
HOGENOMiHOG000246403.
InParanoidiQ21926.
KOiK01870.
OMAiIPLPIWR.
PhylomeDBiQ21926.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q21926-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSGLSTVPDN INFAREEDKV AQKWQDENTF QRSVELSKDR PHFTFYDGPP
60 70 80 90 100
FATGLPHYGH MLTSTIKDVV GRWAHQNGHY VERRFGWDTH GLPVEYEIDK
110 120 130 140 150
TLGISGPQDV MKMGIANYNN ECRKIVMRYS GEWEKTMGRL GRWVDFKHDY
160 170 180 190 200
KTLYPWFMES VWWAFSELHK KGLVYKGVKV MPFSTACSTP LSNFEAGQNY
210 220 230 240 250
KDVVDPAVFV GFKLLDCPNR QLVAWTTTPW TLPSNLALVV HPDMLYVVTK
260 270 280 290 300
DKTTGIEYVV LEERLGELKN DNLEVIEKLA GSQLKDLRYE PLFPYFAYMR
310 320 330 340 350
EERNAFRVLN DTFVTSDSGT GVVHQAPYFG EIDFQVCVAN GVIAKDQKMI
360 370 380 390 400
CPVDESGKYT SEVPDYQGVY VKDADKLIIK RLKEMGNLVR QAEVKHSYPF
410 420 430 440 450
CWRSDTPLLY KAVPSWFINV ETLIPRLLAN NDETYWVPAF VKEKRFANWL
460 470 480 490 500
RDARDWAVSR NRFWGTPINL WVSEDGEEVV CVGSIAELEE LSGQKITDLH
510 520 530 540 550
RESVDDVTIP SRSGRGVLKR VSEVFDCWFE SGSMPYAQNH YPFENRKIFE
560 570 580 590 600
DNFPADFIAE GIDQTRGWFY TLLVLSTALF NKPPFKNLIC NGLVLASDGA
610 620 630 640 650
KMSKSKKNYP DPMLIVNKYG ADALRLYLIN SPVVRGENLR FREEGVRDLL
660 670 680 690 700
KDVFLPWFNA YRFFVQNVQA YEHETGNVFD MNVHVASENV MDRWIESFTN
710 720 730 740 750
SLVAFVRKEM DSYRLYAVVG PLTKFFDTLT NIYIRLNRKR VKGDNGLHEQ
760 770 780 790 800
HHALAALGRV LILIVRLMAP FTPFFCEYIW LNLKKVIGST EESVHFLMLP
810 820 830 840 850
KPDESLIDET VERRVEVMRN VIDLVRLVRD REGLAVKYPL KEMIVINRDS
860 870 880 890 900
QFLEDVKSLE SYILLELNVR KLTVSQDKQK YGITLKAEPN FKILGARLKG
910 920 930 940 950
EQKKVADYLK NQITESELEK FLLEGKLTVL GHELSSEEVA VSYAAGSDQG
960 970 980 990 1000
HGYKTHSDAK TIVMIDTTED ESLIEEGLCR EVTNRVQRLR KQAKLVSTDT
1010 1020 1030 1040 1050
AHVHIVVSPA DSQLAKVVAA KLNDIVSATG TPIKLGSPPA AAKAPTATSK
1060 1070 1080 1090 1100
SAIKDSEVEL WLFAEGDSFD GITVVDGSKK VRVHVKTEKE HLKGYADLLY
1110 1120 1130 1140
HVRSALDLWN GKIALKNVDG SAVHPTVDVT SLVGQTLQLV R
Length:1,141
Mass (Da):130,012
Last modified:November 1, 1996 - v1
Checksum:i151E6AD6D6360EB0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70310 Genomic DNA. Translation: CAA94369.1.
PIRiT24176.
RefSeqiNP_501914.1. NM_069513.4.
UniGeneiCel.17394.

Genome annotation databases

EnsemblMetazoaiR11A8.6.1; R11A8.6.1; WBGene00002152.
R11A8.6.2; R11A8.6.2; WBGene00002152.
GeneIDi177926.
KEGGicel:CELE_R11A8.6.
UCSCiR11A8.6. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70310 Genomic DNA. Translation: CAA94369.1.
PIRiT24176.
RefSeqiNP_501914.1. NM_069513.4.
UniGeneiCel.17394.

3D structure databases

ProteinModelPortaliQ21926.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi43028. 1 interaction.
IntActiQ21926. 1 interaction.
MINTiMINT-114690.
STRINGi6239.R11A8.6.

Proteomic databases

PaxDbiQ21926.
PRIDEiQ21926.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiR11A8.6.1; R11A8.6.1; WBGene00002152.
R11A8.6.2; R11A8.6.2; WBGene00002152.
GeneIDi177926.
KEGGicel:CELE_R11A8.6.
UCSCiR11A8.6. c. elegans.

Organism-specific databases

CTDi177926.
WormBaseiR11A8.6; CE06304; WBGene00002152; iars-1.

Phylogenomic databases

eggNOGiCOG0060.
GeneTreeiENSGT00550000074921.
HOGENOMiHOG000246403.
InParanoidiQ21926.
KOiK01870.
OMAiIPLPIWR.
PhylomeDBiQ21926.

Miscellaneous databases

NextBioi898988.
PROiQ21926.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiSYIC_CAEEL
AccessioniPrimary (citable) accession number: Q21926
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 4, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.