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Protein

NAD-dependent protein deacetylase sir-2.1

Gene

sir-2.1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

NAD-dependent deacetylase (By similarity). Functions upstream of daf-16 in the insulin-like signaling pathway, promoting daf-16 mediated transcriptional activation and increased life-span (PubMed:16777605, PubMed:16280150). May also regulate life-span independently of daf-16 by modulating the transcription of genes involved in the stress response of the endoplasmic reticulum (ER) (PubMed:16256736, PubMed:16280150). Acts upstream of the nicotinic acid metabolism pathway, which may be linked to the regulation of longevity (PubMed:24077178). Plays a role in ascaroside-mediated longevity and stress resistance (PubMed:23509272).By similarity7 Publications

Catalytic activityi

NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.PROSITE-ProRule annotation

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei255Proton acceptorPROSITE-ProRule annotation1
Metal bindingi263ZincPROSITE-ProRule annotation1
Metal bindingi266ZincPROSITE-ProRule annotation1
Metal bindingi287ZincPROSITE-ProRule annotation1
Metal bindingi290ZincPROSITE-ProRule annotation1
Binding sitei369NAD; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi153 – 172NADBy similarityAdd BLAST20
Nucleotide bindingi237 – 240NADBy similarity4
Nucleotide bindingi327 – 329NADBy similarity3
Nucleotide bindingi352 – 354NADBy similarity3

GO - Molecular functioni

  • deacetylase activity Source: WormBase
  • metal ion binding Source: UniProtKB-KW
  • NAD+ binding Source: InterPro

GO - Biological processi

  • dauer larval development Source: WormBase
  • determination of adult lifespan Source: WormBase
  • histone H3 deacetylation Source: WormBase
  • histone H3-K9 acetylation Source: WormBase
  • intrinsic apoptotic signaling pathway in response to DNA damage Source: WormBase
  • protein deacetylation Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

ReactomeiR-CEL-3371453. Regulation of HSF1-mediated heat shock response.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD-dependent protein deacetylase sir-2.1 (EC:3.5.1.-)
Alternative name(s):
Protein sir-2.1
Regulatory protein SIR2 homolog 1
Gene namesi
Name:sir-2.1
ORF Names:R11A8.4
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiR11A8.4a; CE06302; WBGene00004800; sir-2.1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: WormBase
  • nuclear chromosome, telomeric region Source: WormBase
  • nucleus Source: WormBase
  • perinuclear region of cytoplasm Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Reduces the longevity-extending effects of nicotinic acid when exposed to 1 mM nicotinic acid (PubMed:24077178). Defective ascaroside-mediated responses with neither ascr#2 nor ascr#3 inducing lifespan extension or conferring thermotolerance (PubMed:23509272).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002495991 – 607NAD-dependent protein deacetylase sir-2.1Add BLAST607

Proteomic databases

EPDiQ21921.
PaxDbiQ21921.
PRIDEiQ21921.

PTM databases

iPTMnetiQ21921.

Expressioni

Developmental stagei

Expressed in neurons of the head and tail from embryo to adult. Expressed in the hypodermis from the three-fold stage of embryogenesis; expression in the hypodermis subsequently decreases at L3 and is undetectable in adults.1 Publication

Gene expression databases

BgeeiWBGene00004800.
ExpressionAtlasiQ21921. differential.

Interactioni

Subunit structurei

Interacts with ftt-2 and par-5. Interacts with daf-16 following heat-shock, which causes daf-16 to accumulate in the nucleus. Interaction with daf-16 is promoted by ftt-2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
daf-16O168502EBI-966082,EBI-324028
ftt-2Q206553EBI-966082,EBI-966073
par-5P419323EBI-966082,EBI-318108

Protein-protein interaction databases

IntActiQ21921. 3 interactors.
STRINGi6239.R11A8.4a.

Structurei

3D structure databases

ProteinModelPortaliQ21921.
SMRiQ21921.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini136 – 385Deacetylase sirtuin-typePROSITE-ProRule annotationAdd BLAST250

Sequence similaritiesi

Belongs to the sirtuin family. Class I subfamily.Curated
Contains 1 deacetylase sirtuin-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2684. Eukaryota.
COG0846. LUCA.
GeneTreeiENSGT00850000132399.
InParanoidiQ21921.
KOiK11411.
OMAiFSKCTCT.
OrthoDBiEOG091G07CT.
PhylomeDBiQ21921.

Family and domain databases

Gene3Di3.30.1600.10. 2 hits.
3.40.50.1220. 3 hits.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR003000. Sirtuin.
IPR026591. Sirtuin_cat_small_dom.
IPR026590. Ssirtuin_cat_dom.
[Graphical view]
PANTHERiPTHR11085. PTHR11085. 2 hits.
PfamiPF02146. SIR2. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
PROSITEiPS50305. SIRTUIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q21921-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRDSGNDSE VAVTHGEVQE ITEENPEIGS MHITQETDIS DAPETNTDSS
60 70 80 90 100
RQRTESTTSV SSESWQNNDE MMSNLRRAQR LLDDGATPLQ IIQQIFPDFN
110 120 130 140 150
ASRIATMSEN AHFAILSDLL ERAPVRQKLT NYNSLADAVE LFKTKKHILV
160 170 180 190 200
LTGAGVSVSC GIPDFRSKDG IYARLRSEFP DLPDPTAMFD IRYFRENPAP
210 220 230 240 250
FYNFAREIFP GQFVPSVSHR FIKELETSGR LLRNYTQNID TLEHQTGIKR
260 270 280 290 300
VVECHGSFSK CTCTRCGQKY DGNEIREEVL AMRVAHCKRC EGVIKPNIVF
310 320 330 340 350
FGEDLGREFH QHVTEDKHKV DLIVVIGSSL KVRPVALIPH CVDKNVPQIL
360 370 380 390 400
INRESLPHYN ADIELLGNCD DIIRDICFSL GGSFTELITS YDSIMEQQGK
410 420 430 440 450
TKSQKPSQNK RQLISQEDFL NICMKEKRND DSSDEPTLKK PRMSVADDSM
460 470 480 490 500
DSEKNNFQEI QKHKSEDDDD TRNSDDILKK IKHPRLLSIT EMLHDNKCVA
510 520 530 540 550
ISAHQTVFPG AECSFDLETL KLVRDVHHET HCESSCGSSC SSNADSEANQ
560 570 580 590 600
LSRAQSLDDF VLSDEDRKNT IHLDLQRADS CDGDFQYELS ETIDPETFSH

LCEEMRI
Length:607
Mass (Da):68,766
Last modified:November 1, 1996 - v1
Checksum:i71C720BAB41DC88D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70310 Genomic DNA. Translation: CAA94364.1.
PIRiT24172.
RefSeqiNP_001255484.1. NM_001268555.1.
UniGeneiCel.12479.

Genome annotation databases

EnsemblMetazoaiR11A8.4a; R11A8.4a; WBGene00004800.
GeneIDi177924.
KEGGicel:CELE_R11A8.4.
UCSCiR11A8.4.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70310 Genomic DNA. Translation: CAA94364.1.
PIRiT24172.
RefSeqiNP_001255484.1. NM_001268555.1.
UniGeneiCel.12479.

3D structure databases

ProteinModelPortaliQ21921.
SMRiQ21921.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ21921. 3 interactors.
STRINGi6239.R11A8.4a.

PTM databases

iPTMnetiQ21921.

Proteomic databases

EPDiQ21921.
PaxDbiQ21921.
PRIDEiQ21921.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiR11A8.4a; R11A8.4a; WBGene00004800.
GeneIDi177924.
KEGGicel:CELE_R11A8.4.
UCSCiR11A8.4.1. c. elegans.

Organism-specific databases

CTDi177924.
WormBaseiR11A8.4a; CE06302; WBGene00004800; sir-2.1.

Phylogenomic databases

eggNOGiKOG2684. Eukaryota.
COG0846. LUCA.
GeneTreeiENSGT00850000132399.
InParanoidiQ21921.
KOiK11411.
OMAiFSKCTCT.
OrthoDBiEOG091G07CT.
PhylomeDBiQ21921.

Enzyme and pathway databases

ReactomeiR-CEL-3371453. Regulation of HSF1-mediated heat shock response.

Miscellaneous databases

PROiQ21921.

Gene expression databases

BgeeiWBGene00004800.
ExpressionAtlasiQ21921. differential.

Family and domain databases

Gene3Di3.30.1600.10. 2 hits.
3.40.50.1220. 3 hits.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR003000. Sirtuin.
IPR026591. Sirtuin_cat_small_dom.
IPR026590. Ssirtuin_cat_dom.
[Graphical view]
PANTHERiPTHR11085. PTHR11085. 2 hits.
PfamiPF02146. SIR2. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
PROSITEiPS50305. SIRTUIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSIR2_CAEEL
AccessioniPrimary (citable) accession number: Q21921
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.