Q21902 (MCM5_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA replication licensing factor mcm-5
EC=3.6.4.12

Gene names

Name:	mcm-5
ORF Names:	R10E4.4

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	759 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Acts as component of the mcm2-7 complex (mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Component of the mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order mcm2-mcm6-mcm4-mcm7-mcm3-mcm5 (By simililarity).
Subcellular location	Nucleus Potential.
Sequence similarities	Belongs to the MCM family. Contains 1 MCM domain.

Ontologies

Keywords
Biological process	Cell cycle DNA replication
Cellular component	Nucleus
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA duplex unwinding Inferred from electronic annotation. Source: GOC DNA replication initiation Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	MCM complex Inferred from electronic annotation. Source: InterPro nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA helicase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 759	759	DNA replication licensing factor mcm-5		PRO_0000194110
Regions
	Domain	330 – 536	207	MCM
	Nucleotide binding	380 – 387	8	ATP Potential
	Motif	511 – 514	4	Arginine finger

Sequences

	Sequence		Length	Mass (Da)
	Q21902 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 8D81B2AB5090A92B	FASTA	759	84,936
	10 20 30 40 50 60 MSNLDNPGIY YQERFFANDG VPDTGRELIA EYRQLITQFR NFIRDFSTGG FGMIYRDQLK 70 80 90 100 110 120 RNYFSHEYRL EINLNHLKNF DEDIEMKLRK FPGKVLPALE EAAKIVADEI TTPRPKGEEK 130 140 150 160 170 180 LHDIQVTLTL DEYPTSLRQV KSAQVSQVVK ISGIIVAAAQ VRSKATKVTL QCRQCKHTIP 190 200 210 220 230 240 DVSIKPGLEG FALPRTCAAP QQGQMQRCPI DPYIMLPDKC ECVDYQTLKL QENPEDVPHG 250 260 270 280 290 300 EMPRHLQLFT ERYLTDKVVP GNRVTIVGVY SIKKLIQKKG GDKSLQGIRT PYLRVLGIHM 310 320 330 340 350 360 ETSGPGRTNF TTFTPEEERM FKTLAQRKDA YELIAKSIAP SIYGSADIKK SIACLLFGGA 370 380 390 400 410 420 RKKLPDGITR RGDINVLLLG DPGTAKSQLL KFVEQVSPIG VYTSGKGSSA AGLTASVIRD 430 440 450 460 470 480 PQSRSFIMEG GAMVLADGGV VCIDEFDKMR EDDRVAIHEA MEQQTISIAK AGITTTLNSR 490 500 510 520 530 540 CSVLAAANSV YGRWDESRGD DNIDFMPTIL SRFDMIYIVK DTHDVLKDAT LAKHVIEVHV 550 560 570 580 590 600 NASAAKERDI AGVPKTATTD SDGVMTMFDT DGFLTIEFLK KFVTYARLNC GPRLTPQASE 610 620 630 640 650 660 KLVNHYVKMR NPVVNADAFK SGKKAHNSAI PITVRQLEAI VRIAESIAKM ELQQFATDKH 670 680 690 700 710 720 VEEALRLFRV STIEAAATGN LAGVEGFTST ADQEALNRIE VQMKKRFAIG THVSEHLIVQ 730 740 750 DFVARQHYRE SLVKKVIDNL VRRGDLQQKM QRKMLYRVR

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z50874 Genomic DNA. Translation: CAA90765.1.
PIR	T24130.
RefSeq	NP_497858.1. NM_065457.4.
3D structure databases
ProteinModelPortal	Q21902.
SMR	Q21902. Positions 70-667.
ModBase	Search...
Protein-protein interaction databases
STRING	6239.R10E4.4.2.
Proteomic databases
PaxDb	Q21902.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	R10E4.4.1; R10E4.4.1; R10E4.4. R10E4.4.2; R10E4.4.2; R10E4.4.
GeneID	175552.
KEGG	cel:CELE_R10E4.4.
UCSC	R10E4.4.1. c. elegans.
Organism-specific databases
CTD	175552.
WormBase	R10E4.4; CE03558; WBGene00003157; mcm-5.
Phylogenomic databases
eggNOG	COG1241.
GeneTree	ENSGT00550000074928.
HOGENOM	HOG000224128.
InParanoid	Q21902.
KO	K02209.
OMA	KKSIACL.
Family and domain databases
Gene3D	2.40.50.140. 2 hits.
InterPro	IPR008048. MCM5. IPR018525. MCM_CS. IPR001208. MCM_DNA-dep_ATPase. IPR012340. NA-bd_OB-fold. [Graphical view]
Pfam	PF00493. MCM. 1 hit. [Graphical view]
PRINTS	PR01657. MCMFAMILY. PR01661. MCMPROTEIN5.
SMART	SM00350. MCM. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
PROSITE	PS00847. MCM_1. 1 hit. PS50051. MCM_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	888644.

Entry information

Entry name

MCM5_CAEEL

Accession

Primary (citable) accession number: Q21902

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project