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Protein

DNA replication licensing factor mcm-5

Gene

mcm-5

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Acts as component of the mcm2-7 complex (mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi380 – 3878ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA helicase activity Source: InterPro

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. DNA replication initiation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_238820. Unwinding of DNA.
REACT_244143. Removal of licensing factors from origins.
REACT_255129. Switching of origins to a post-replicative state.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor mcm-5 (EC:3.6.4.12)
Gene namesi
Name:mcm-5
ORF Names:R10E4.4
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome III

Organism-specific databases

WormBaseiR10E4.4; CE03558; WBGene00003157; mcm-5.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. MCM complex Source: InterPro
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 759759DNA replication licensing factor mcm-5PRO_0000194110Add
BLAST

Proteomic databases

PaxDbiQ21902.

Interactioni

Subunit structurei

Component of the mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order mcm2-mcm6-mcm4-mcm7-mcm3-mcm5 (By simililarity).

Protein-protein interaction databases

BioGridi40789. 1 interaction.
STRINGi6239.R10E4.4.2.

Structurei

3D structure databases

ProteinModelPortaliQ21902.
SMRiQ21902. Positions 34-670.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini330 – 536207MCMAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi511 – 5144Arginine finger

Sequence similaritiesi

Belongs to the MCM family.Curated
Contains 1 MCM domain.Curated

Phylogenomic databases

eggNOGiCOG1241.
GeneTreeiENSGT00550000074928.
HOGENOMiHOG000224128.
InParanoidiQ21902.
KOiK02209.
OMAiHVIEVHV.
PhylomeDBiQ21902.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR008048. MCM5.
IPR018525. MCM_CS.
IPR001208. MCM_DNA-dep_ATPase.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01661. MCMPROTEIN5.
SMARTiSM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q21902-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNLDNPGIY YQERFFANDG VPDTGRELIA EYRQLITQFR NFIRDFSTGG
60 70 80 90 100
FGMIYRDQLK RNYFSHEYRL EINLNHLKNF DEDIEMKLRK FPGKVLPALE
110 120 130 140 150
EAAKIVADEI TTPRPKGEEK LHDIQVTLTL DEYPTSLRQV KSAQVSQVVK
160 170 180 190 200
ISGIIVAAAQ VRSKATKVTL QCRQCKHTIP DVSIKPGLEG FALPRTCAAP
210 220 230 240 250
QQGQMQRCPI DPYIMLPDKC ECVDYQTLKL QENPEDVPHG EMPRHLQLFT
260 270 280 290 300
ERYLTDKVVP GNRVTIVGVY SIKKLIQKKG GDKSLQGIRT PYLRVLGIHM
310 320 330 340 350
ETSGPGRTNF TTFTPEEERM FKTLAQRKDA YELIAKSIAP SIYGSADIKK
360 370 380 390 400
SIACLLFGGA RKKLPDGITR RGDINVLLLG DPGTAKSQLL KFVEQVSPIG
410 420 430 440 450
VYTSGKGSSA AGLTASVIRD PQSRSFIMEG GAMVLADGGV VCIDEFDKMR
460 470 480 490 500
EDDRVAIHEA MEQQTISIAK AGITTTLNSR CSVLAAANSV YGRWDESRGD
510 520 530 540 550
DNIDFMPTIL SRFDMIYIVK DTHDVLKDAT LAKHVIEVHV NASAAKERDI
560 570 580 590 600
AGVPKTATTD SDGVMTMFDT DGFLTIEFLK KFVTYARLNC GPRLTPQASE
610 620 630 640 650
KLVNHYVKMR NPVVNADAFK SGKKAHNSAI PITVRQLEAI VRIAESIAKM
660 670 680 690 700
ELQQFATDKH VEEALRLFRV STIEAAATGN LAGVEGFTST ADQEALNRIE
710 720 730 740 750
VQMKKRFAIG THVSEHLIVQ DFVARQHYRE SLVKKVIDNL VRRGDLQQKM

QRKMLYRVR
Length:759
Mass (Da):84,936
Last modified:November 1, 1997 - v1
Checksum:i8D81B2AB5090A92B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z50874 Genomic DNA. Translation: CAA90765.1.
PIRiT24130.
RefSeqiNP_497858.1. NM_065457.4.
UniGeneiCel.10253.

Genome annotation databases

EnsemblMetazoaiR10E4.4.1; R10E4.4.1; WBGene00003157.
R10E4.4.2; R10E4.4.2; WBGene00003157.
GeneIDi175552.
KEGGicel:CELE_R10E4.4.
UCSCiR10E4.4.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z50874 Genomic DNA. Translation: CAA90765.1.
PIRiT24130.
RefSeqiNP_497858.1. NM_065457.4.
UniGeneiCel.10253.

3D structure databases

ProteinModelPortaliQ21902.
SMRiQ21902. Positions 34-670.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi40789. 1 interaction.
STRINGi6239.R10E4.4.2.

Proteomic databases

PaxDbiQ21902.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiR10E4.4.1; R10E4.4.1; WBGene00003157.
R10E4.4.2; R10E4.4.2; WBGene00003157.
GeneIDi175552.
KEGGicel:CELE_R10E4.4.
UCSCiR10E4.4.1. c. elegans.

Organism-specific databases

CTDi175552.
WormBaseiR10E4.4; CE03558; WBGene00003157; mcm-5.

Phylogenomic databases

eggNOGiCOG1241.
GeneTreeiENSGT00550000074928.
HOGENOMiHOG000224128.
InParanoidiQ21902.
KOiK02209.
OMAiHVIEVHV.
PhylomeDBiQ21902.

Enzyme and pathway databases

ReactomeiREACT_238820. Unwinding of DNA.
REACT_244143. Removal of licensing factors from origins.
REACT_255129. Switching of origins to a post-replicative state.

Miscellaneous databases

NextBioi888644.
PROiQ21902.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR008048. MCM5.
IPR018525. MCM_CS.
IPR001208. MCM_DNA-dep_ATPase.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01661. MCMPROTEIN5.
SMARTiSM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiMCM5_CAEEL
AccessioniPrimary (citable) accession number: Q21902
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.