Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q21902 (MCM5_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA replication licensing factor mcm-5

EC=3.6.4.12
Gene names
Name:mcm-5
ORF Names:R10E4.4
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length759 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Acts as component of the mcm2-7 complex (mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity By similarity.

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Component of the mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order mcm2-mcm6-mcm4-mcm7-mcm3-mcm5 (By simililarity).

Subcellular location

Nucleus Potential.

Sequence similarities

Belongs to the MCM family.

Contains 1 MCM domain.

Ontologies

Keywords
   Biological processCell cycle
DNA replication
   Cellular componentNucleus
   LigandATP-binding
DNA-binding
Nucleotide-binding
   Molecular functionHelicase
Hydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication initiation

Inferred from electronic annotation. Source: InterPro

cell cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentMCM complex

Inferred from electronic annotation. Source: InterPro

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA helicase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 759759DNA replication licensing factor mcm-5
PRO_0000194110

Regions

Domain330 – 536207MCM
Nucleotide binding380 – 3878ATP Potential
Motif511 – 5144Arginine finger

Sequences

Sequence LengthMass (Da)Tools
Q21902 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 8D81B2AB5090A92B

FASTA75984,936
        10         20         30         40         50         60 
MSNLDNPGIY YQERFFANDG VPDTGRELIA EYRQLITQFR NFIRDFSTGG FGMIYRDQLK 

        70         80         90        100        110        120 
RNYFSHEYRL EINLNHLKNF DEDIEMKLRK FPGKVLPALE EAAKIVADEI TTPRPKGEEK 

       130        140        150        160        170        180 
LHDIQVTLTL DEYPTSLRQV KSAQVSQVVK ISGIIVAAAQ VRSKATKVTL QCRQCKHTIP 

       190        200        210        220        230        240 
DVSIKPGLEG FALPRTCAAP QQGQMQRCPI DPYIMLPDKC ECVDYQTLKL QENPEDVPHG 

       250        260        270        280        290        300 
EMPRHLQLFT ERYLTDKVVP GNRVTIVGVY SIKKLIQKKG GDKSLQGIRT PYLRVLGIHM 

       310        320        330        340        350        360 
ETSGPGRTNF TTFTPEEERM FKTLAQRKDA YELIAKSIAP SIYGSADIKK SIACLLFGGA 

       370        380        390        400        410        420 
RKKLPDGITR RGDINVLLLG DPGTAKSQLL KFVEQVSPIG VYTSGKGSSA AGLTASVIRD 

       430        440        450        460        470        480 
PQSRSFIMEG GAMVLADGGV VCIDEFDKMR EDDRVAIHEA MEQQTISIAK AGITTTLNSR 

       490        500        510        520        530        540 
CSVLAAANSV YGRWDESRGD DNIDFMPTIL SRFDMIYIVK DTHDVLKDAT LAKHVIEVHV 

       550        560        570        580        590        600 
NASAAKERDI AGVPKTATTD SDGVMTMFDT DGFLTIEFLK KFVTYARLNC GPRLTPQASE 

       610        620        630        640        650        660 
KLVNHYVKMR NPVVNADAFK SGKKAHNSAI PITVRQLEAI VRIAESIAKM ELQQFATDKH 

       670        680        690        700        710        720 
VEEALRLFRV STIEAAATGN LAGVEGFTST ADQEALNRIE VQMKKRFAIG THVSEHLIVQ 

       730        740        750 
DFVARQHYRE SLVKKVIDNL VRRGDLQQKM QRKMLYRVR 

« Hide

References

[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z50874 Genomic DNA. Translation: CAA90765.1.
PIRT24130.
RefSeqNP_497858.1. NM_065457.4.
UniGeneCel.10253.

3D structure databases

ProteinModelPortalQ21902.
SMRQ21902. Positions 34-673.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid40789. 1 interaction.
STRING6239.R10E4.4.2.

Proteomic databases

PaxDbQ21902.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaR10E4.4.1; R10E4.4.1; R10E4.4.
R10E4.4.2; R10E4.4.2; R10E4.4.
GeneID175552.
KEGGcel:CELE_R10E4.4.
UCSCR10E4.4.1. c. elegans.

Organism-specific databases

CTD175552.
WormBaseR10E4.4; CE03558; WBGene00003157; mcm-5.

Phylogenomic databases

eggNOGCOG1241.
HOGENOMHOG000224128.
InParanoidQ21902.
KOK02209.
OMAIVKDTHD.
PhylomeDBQ21902.

Family and domain databases

Gene3D2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProIPR008048. MCM5.
IPR018525. MCM_CS.
IPR001208. MCM_DNA-dep_ATPase.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSPR01657. MCMFAMILY.
PR01661. MCMPROTEIN5.
SMARTSM00350. MCM. 1 hit.
[Graphical view]
SUPFAMSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio888644.
PROQ21902.

Entry information

Entry nameMCM5_CAEEL
AccessionPrimary (citable) accession number: Q21902
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase