Reviewed, UniProtKB/Swiss-Prot Q21815 (MIG23_CAEEL)

Names and origin

Protein names

Recommended name:
    Nucleoside-diphosphatase mig-23
      Short name=NDPase
    EC=3.6.1.6
Alternative name(s):
    Abnormal cell migration protein 23

Gene names

Name:	mig-23
ORF Names:	R07E4.4

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	552 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Seems to be able to hydrolyze ADP, UDP and GDP. Supports mig-17 glycosylation and surface expression, which is required for proper migration of distal tip cells during gonad morphogenesis. Ref.1
Catalytic activity	A nucleoside diphosphate + H2O = a nucleotide + phosphate.
Subcellular location	Golgi apparatus membrane; Multi-pass membrane protein. Ref.1 Ref.4
Tissue specificity	Expressed in body wall muscles. Ref.1
Induction	In contrast to uda-1, expression is not induced by stress. Ref.3
Sequence similarities	Belongs to the GDA1/CD39 NTPase family.

Ontologies

Keywords
Biological process	Differentiation Gonadal differentiation
Cellular component	Golgi apparatus Membrane
Domain	Transmembrane
Molecular function	Developmental protein Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW gonadal mesoderm development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	nucleoside-diphosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 552	552	Nucleoside-diphosphatase mig-23		PRO_0000209923
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 27	21	Potential
	Topological domain	28 – 489	462	Lumenal Potential
	Transmembrane	490 – 510	21	Potential
	Topological domain	511 – 552	42	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	190	1	N-linked (GlcNAc...) Potential
	Glycosylation	284	1	N-linked (GlcNAc...) Potential
Experimental info
	Mutagenesis	268	1	G → E in k166; reduced migration of the gonad arms in both the anterior and posterior direction. Ref.1
	Mutagenesis	290	1	D → N in k146; reduced migration of the gonad arms in both the anterior and posterior direction. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q21815-1 [UniParc]. Last modified June 6, 2002. Version 2. Checksum: E5DC32C858AE4D94	FASTA	552	62,511
	10 20 30 40 50 60 MRVSLRFTIL AVSAMIFFPV IVFIYVVEAH TSPKVIADDQ ERSYGVICDA GSTGTRLFVY 70 80 90 100 110 120 NWISTSDSEL IQIEPVIYDN KPVMKKISPG LSTFGTKPAQ AAEYLRPLME LAERHIPEEK 130 140 150 160 170 180 RPYTPVFIFA TAGMRLIPDE QKEAVLKNLR NKLPKITSMQ VLKEHIRIIE GKWEGIYSWI 190 200 210 220 230 240 AVNYALGKFN KTATLDFPGT SPAHARQKTV GMIDMGGASA QIAFELPDTD SFSSINVENI 250 260 270 280 290 300 NLGCREDDSL FKYKLFVTTF LGYGVNEGIR KYEHMLLSKL KDQNGTVIQD DCMPLNLHKT 310 320 330 340 350 360 VTLENGENFV RRGTGNWNTC SNEVKKLLNP ESSSEVCKAE AAKCYFGAVP APSIPLSNIE 370 380 390 400 410 420 MYGFSEYWYS THDVLGLGGQ YDAENIAKKT QQYCSKRWST IQAESKKQLY PRADEERLRT 430 440 450 460 470 480 QCFKSAWITS VLHDGFSVDK THNKFQSVST IAGQEVQWAL GAMIYHMRFF PLRDSSRNLI 490 500 510 520 530 540 VKETHSSSES LWAPLFFLSA VFCLFVLVCA KEQSVLCFDD KRRSSFGMSR SQYSYKMLKE 550 NRTSSSFLEN FA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An NDPase links ADAM protease glycosylation with organ morphogenesis in C. elegans." Nishiwaki K., Kubota Y., Chigira Y., Roy S.K., Suzuki M., Schvarzstein M., Jigami Y., Hisamoto N., Matsumoto K. Nat. Cell Biol. 6:31-37(2004) [PubMed: 14688791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF GLY-268 AND ASP-290.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"ire-1-dependent transcriptional up-regulation of a lumenal uridine diphosphatase from Caenorhabditis elegans." Uccelletti D., O'Callaghan C., Berninsone P., Zemtseva I., Abeijon C., Hirschberg C.B. J. Biol. Chem. 279:27390-27398(2004) [PubMed: 15102851] [Abstract] Cited for: LACK OF INDUCTION BY STRESS.
[4]	"The conserved oligomeric Golgi complex acts in organ morphogenesis via glycosylation of an ADAM protease in C. elegans." Kubota Y., Sano M., Goda S., Suzuki N., Nishiwaki K. Development 133:263-273(2006) [PubMed: 16354716] [Abstract] Cited for: SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB126261 mRNA. Translation: BAD02168.1. U39652 Genomic DNA. Translation: AAA80403.2.
PIR	T16696.
RefSeq	NP_508994.1.
UniGene	Cel.6413
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	R07E4.4. Caenorhabditis elegans. [Contig view]
GeneID	180860.
KEGG	cel:R07E4.4.
Organism-specific databases
WormBase	WBGene00003254. mig-23.
WormPep	R07E4.4. CE28748. [WorfDB]
Phylogenomic databases
OMA	Q21815. SAWITSV.
Enzyme and pathway databases
BRENDA	3.6.1.6. 672.
Gene expression databases
ArrayExpress	Q21815.
Family and domain databases
InterPro	IPR000407. GDA1_CD39_NTPase. [Graphical view]
PANTHER	PTHR11782. GDA1_CD39_NTPase. 1 hit.
Pfam	PF01150. GDA1_CD39. 1 hit. [Graphical view]
PROSITE	PS01238. GDA1_CD39_NTPASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	911292.

Entry information

Entry name

MIG23_CAEEL

Accession

Primary (citable) accession number: Q21815

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	June 6, 2002
Last modified:	June 16, 2009
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project