Reviewed, UniProtKB/Swiss-Prot Q21774 (PUR8_CAEEL)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate lyase
      Short name=ASL
    EC=4.3.2.2
Alternative name(s):
    Adenylosuccinase
      Short name=ASase

Gene names

ORF Names:

R06C7.5

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	N(6)-(1,2-dicarboxyethyl)AMP = fumarate + AMP. (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2. Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 5/5.
Subunit structure	Homotetramer. Ref.2
Sequence similarities	Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.

Ontologies

Keywords
Biological process	Purine biosynthesis
Coding sequence diversity	Alternative splicing
Molecular function	Lyase
PTM	Disulfide bond
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	body morphogenesis Inferred from mutant phenotype. Source: WormBase embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase hermaphrodite genitalia development Inferred from mutant phenotype. Source: WormBase locomotion Inferred from mutant phenotype. Source: WormBase morphogenesis of an epithelium Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase nucleus organization Inferred from mutant phenotype. Source: WormBase oviposition Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase purine ribonucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate AMP-lyase (fumarate-forming) activity Inferred from electronic annotation. Source: EC N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: Q21774-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: Q21774-2)

The sequence of this isoform differs from the canonical sequence as follows:
     353-370: NIFEGLSVQTDNVKKIVE → VSVLKITSSKDLVYKQTM
     371-478: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 478

478

Adenylosuccinate lyase

PRO_0000137897

Sites

Active site

82

1

Proton donor By similarity

Active site

155

1

Proton acceptor By similarity

Amino acid modifications

Disulfide bond

262 ↔ 300

Potential

Natural variations

Alternative sequence

353 – 370

18

NIFEG…KKIVE → VSVLKITSSKDLVYKQTM in isoform b.

VSP_016396

Alternative sequence

371 – 478

108

Missing in isoform b.

VSP_016397

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

478

Helix Strand Turn

Details...

Helix

3 – 5

3

Helix

10 – 13

4

Turn

14 – 18

5

Helix

21 – 24

4

Helix

27 – 47

21

Helix

55 – 63

9

Turn

64 – 66

3

Helix

70 – 79

10

Helix

83 – 94

12

Turn

96 – 98

3

Helix

99 – 101

3

Turn

102 – 105

4

Helix

108 – 144

37

Turn

145 – 147

3

Beta strand

149 – 154

6

Beta strand

157 – 163

7

Helix

164 – 188

25

Turn

196 – 198

3

Helix

202 – 207

6

Turn

208 – 210

3

Helix

212 – 225

14

Beta strand

236 – 238

3

Helix

242 – 270

29

Beta strand

273 – 275

3

Helix

295 – 308

14

Helix

311 – 319

9

Helix

327 – 329

3

Helix

330 – 357

28

Helix

362 – 384

23

Turn

422 – 425

4

Turn

427 – 429

3

Helix

430 – 438

9

Helix

440 – 443

4

Helix

447 – 457

11

Helix

459 – 463

5

Helix

464 – 466

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 0E7FAA122D32774F	FASTA	478	53,569
	10 20 30 40 50 60 MASEDKFESV LSTRYCKNSP LVSILSETNK ATLWRQLWIW LAEAEKELGL KQVTQDAIDE 70 80 90 100 110 120 MKSNRDVFDW PFIRSEERKL KHDVMAHNHA FGKLCPTAAG IIHLGATSCF VQDNADLIAY 130 140 150 160 170 180 RDSIDHILKR FATVIDRLAA FSLKNKEVVT VGRTHYQTAS LVTVGKRGVL WAQELLMAFQ 190 200 210 220 230 240 SLSEFRDKMR FRGIKGATGT QDSFLTLFAG DESKVEALDE LVTKKANFSN RFLITGQTYS 250 260 270 280 290 300 RQQDSQLVFS LSLLGAAAKK VCTDIRVLQA FGELLEPFEK DQIGSSAMPY KKNPMKSERC 310 320 330 340 350 360 CALSRKLINA PQEALTILAD QGLERTLDDS AGRRMLIPDV LLTAEALLTT LQNIFEGLSV 370 380 390 400 410 420 QTDNVKKIVE DEIAFLGLEK AMMMLTEEGV DRQQAHAVIR KTALEAKQLQ ATQKVDIRQT 430 440 450 460 470 MADPFFDSVR DRVVGLVNNP INFTGRCVSQ TESFIAKELK PTIDKYLDKS AGNVQLDV
	Isoform b. Checksum: C803E93C6C048BA1	FASTA	370	41,496
	10 20 30 40 50 60 MASEDKFESV LSTRYCKNSP LVSILSETNK ATLWRQLWIW LAEAEKELGL KQVTQDAIDE 70 80 90 100 110 120 MKSNRDVFDW PFIRSEERKL KHDVMAHNHA FGKLCPTAAG IIHLGATSCF VQDNADLIAY 130 140 150 160 170 180 RDSIDHILKR FATVIDRLAA FSLKNKEVVT VGRTHYQTAS LVTVGKRGVL WAQELLMAFQ 190 200 210 220 230 240 SLSEFRDKMR FRGIKGATGT QDSFLTLFAG DESKVEALDE LVTKKANFSN RFLITGQTYS 250 260 270 280 290 300 RQQDSQLVFS LSLLGAAAKK VCTDIRVLQA FGELLEPFEK DQIGSSAMPY KKNPMKSERC 310 320 330 340 350 360 CALSRKLINA PQEALTILAD QGLERTLDDS AGRRMLIPDV LLTAEALLTT LQVSVLKITS 370 SKDLVYKQTM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
[2]	"Structural genomics of Caenorhabditis elegans: adenylosuccinate lyase." Southeast collaboratory for structural genomics (SECSG) Submitted (FEB-2005) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS), POTENTIAL DISULFIDE BOND, SUBUNIT.

Cross-references

Sequence databases

EMBLGenBankDDBJ

Z71266 Genomic DNA. Translation: CAA95843.1.
Z71266 Genomic DNA. Translation: CAD56593.1.

PIR

T23969.

RefSeq

NP_492049.1.

UniGene

Cel.157

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YIS	X-ray	2.40	A	1-478	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q21774. 3 interactions.

Proteomic databases

PRIDE

Q21774.

Genome annotation databases

Ensembl

R06C7.5. Caenorhabditis elegans. [Contig view]

GeneID

172466.

KEGG

cel:R06C7.5.

Organism-specific databases

WormBase

WBGene00011064. R06C7.5.

WormPep

R06C7.5a. CE06248. [WorfDB]
R06C7.5b. CE32476. [WorfDB]

Phylogenomic databases

OMA

Q21774. NRQDCHE.

Enzyme and pathway databases

BRENDA

4.3.2.2. 672.

Gene expression databases

ArrayExpress

Q21774.

Family and domain databases

InterPro

IPR000362. Fumarate_lyase.
IPR004769. Pur_lyase.
[Graphical view]

Pfam

PF00206. Lyase_1. 1 hit.
[Graphical view]

PRINTS

PR00149. FUMRATELYASE.

TIGRFAMs

TIGR00928. purB. 1 hit.

PROSITE

PS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

875631.

Entry information

Entry name

PUR8_CAEEL

Accession

Primary (citable) accession number: Q21774
Secondary accession number(s): Q8I4G8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project