ID   ASPG_CAEEL              Reviewed;         363 AA.
AC   Q21697;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   06-JUN-2002, sequence version 2.
DT   16-JUN-2009, entry version 57.
DE   RecName: Full=Putative N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase;
DE            EC=3.5.1.26;
DE   AltName: Full=Glycosylasparaginase;
DE   AltName: Full=Aspartylglucosaminidase;
DE            Short=AGA;
DE   AltName: Full=N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase;
DE   Contains:
DE     RecName: Full=Glycosylasparaginase alpha chain;
DE   Contains:
DE     RecName: Full=Glycosylasparaginase beta chain;
DE   Flags: Precursor;
GN   ORFNames=R04B3.2;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Nematoda; Chromadorea; Rhabditida; Rhabditoidea;
OC   Rhabditidae; Peloderinae; Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   MEDLINE=99069613; PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for
RT   investigating biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: Cleaves the GlcNAc-Asn bond which joins oligosaccharides
CC       to the peptide of asparagine-linked glycoproteins (By similarity).
CC   -!- CATALYTIC ACTIVITY: N(4)-(beta-N-acetyl-D-glucosaminyl)-L-
CC       asparagine + H(2)O = N-acetyl-beta-D-glucosaminylamine + L-
CC       aspartate.
CC   -!- SUBUNIT: Heterotetramer of two alpha and two beta chains arranged
CC       as a dimer of alpha/beta heterodimers (By similarity).
CC   -!- SUBCELLULAR LOCATION: Lysosome (By similarity).
CC   -!- PTM: Autocleaved. Generates the alpha and beta subunits. The N-
CC       terminal residue of the beta subunit is thought to be responsible
CC       for the nucleophile hydrolase activity (By similarity).
CC   -!- SIMILARITY: Belongs to the Ntn-hydrolase family.
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DR   EMBL; U50198; AAA91260.2; -; Genomic_DNA.
DR   PIR; T30965; T30965.
DR   RefSeq; NP_508448.1; -.
DR   UniGene; Cel.593; -.
DR   HSSP; P20933; 1APY.
DR   MEROPS; T02.001; -.
DR   Ensembl; R04B3.2; Caenorhabditis elegans.
DR   GeneID; 180550; -.
DR   KEGG; cel:R04B3.2; -.
DR   NMPDR; fig|6239.3.peg.22677; -.
DR   WormBase; WBGene00019867; R04B3.2.
DR   WormPep; R04B3.2; CE28745.
DR   OMA; Q21697; PLVLNTW.
DR   BRENDA; 3.5.1.26; 672.
DR   NextBio; 909836; -.
DR   ArrayExpress; Q21697; -.
DR   GO; GO:0005764; C:lysosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0003948; F:N4-(beta-N-acetylglucosaminyl)-L-asparagina...; IEA:EC.
DR   InterPro; IPR000246; Peptidase_T2.
DR   PANTHER; PTHR10188; Peptidase_T2; 1.
DR   Pfam; PF01112; Asparaginase_2; 1.
PE   2: Evidence at transcript level;
KW   Complete proteome; Disulfide bond; Glycoprotein; Hydrolase; Lysosome;
KW   Signal.
FT   SIGNAL        1     17       Potential.
FT   CHAIN        18    211       Glycosylasparaginase alpha chain (By
FT                                similarity).
FT                                /FTId=PRO_0000002343.
FT   CHAIN       212    363       Glycosylasparaginase beta chain (By
FT                                similarity).
FT                                /FTId=PRO_0000002344.
FT   ACT_SITE    212    212       Nucleophile (By similarity).
FT   CARBOHYD     15     15       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    118    118       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    196    196       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    336    336       N-linked (GlcNAc...) (Potential).
FT   DISULFID    161    177       By similarity.
FT   DISULFID    322    346       By similarity.
SQ   SEQUENCE   363 AA;  39359 MW;  4A34D1450AAB1BC7 CRC64;
     MRRFYIFLLL IPYINGTIND DSLPMVITTW GSDGFKKATK NAVDATLLGG RMFGLVEGLS
     TCEALQCDTT VGYGGSPDEN GETCLDSLVI DADGMRVGAV ANLHRIRDAA RVAWGVMNFT
     KHTLLVGESA TQFAKTLGFK EEDLSTEETK SWISKWKTEK CQPNFWKNVS PDPSSSCGPY
     KTNPLTKSMR YYSLVNQSDE AGYLVEKTNH DTIGMVVRDT ENIFSAGTSS NGARFKIPGR
     VGDSPIPGAG AYANKFGGAA ATGDGDVMMR FLPSFFAVTQ MELGTKPSKA AYKAITRILK
     VFPKFSGAVV AMNVKGRIGA SCANINKFGY NVAFQNGTVV TYSISCLKEV NSLKYLKEGI
     EFA
//