Reviewed, UniProtKB/Swiss-Prot Q21697 (ASPG_CAEEL)

Names and origin

Protein names

Recommended name:
    Putative N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase
    EC=3.5.1.26
Alternative name(s):
    Glycosylasparaginase
    Aspartylglucosaminidase
      Short name=AGA
    N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase
Cleaved into the following 2 chains:
    1- Recommended name:
            Glycosylasparaginase alpha chain
    2- Recommended name:
            Glycosylasparaginase beta chain

Gene names

ORF Names:

R04B3.2

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cleaves the GlcNAc-Asn bond which joins oligosaccharides to the peptide of asparagine-linked glycoproteins By similarity.
Catalytic activity	N(4)-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H2O = N-acetyl-beta-D-glucosaminylamine + L-aspartate.
Subunit structure	Heterotetramer of two alpha and two beta chains arranged as a dimer of alpha/beta heterodimers By similarity.
Subcellular location	Lysosome By similarity.
Post-translational modification	Autocleaved. Generates the alpha and beta subunits. The N-terminal residue of the beta subunit is thought to be responsible for the nucleophile hydrolase activity By similarity.
Sequence similarities	Belongs to the Ntn-hydrolase family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N4-(beta-N-acetylglucosaminyl)-L-asparaginase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 211	194	Glycosylasparaginase alpha chain By similarity		PRO_0000002343
	Chain	212 – 363	152	Glycosylasparaginase beta chain By similarity		PRO_0000002344
Sites
	Active site	212	1	Nucleophile By similarity
Amino acid modifications
	Glycosylation	15	1	N-linked (GlcNAc...) Potential
	Glycosylation	118	1	N-linked (GlcNAc...) Potential
	Glycosylation	196	1	N-linked (GlcNAc...) Potential
	Glycosylation	336	1	N-linked (GlcNAc...) Potential
	Disulfide bond	161 ↔ 177		By similarity
	Disulfide bond	322 ↔ 346		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q21697-1 [UniParc]. Last modified June 6, 2002. Version 2. Checksum: 4A34D1450AAB1BC7	FASTA	363	39,359
	10 20 30 40 50 60 MRRFYIFLLL IPYINGTIND DSLPMVITTW GSDGFKKATK NAVDATLLGG RMFGLVEGLS 70 80 90 100 110 120 TCEALQCDTT VGYGGSPDEN GETCLDSLVI DADGMRVGAV ANLHRIRDAA RVAWGVMNFT 130 140 150 160 170 180 KHTLLVGESA TQFAKTLGFK EEDLSTEETK SWISKWKTEK CQPNFWKNVS PDPSSSCGPY 190 200 210 220 230 240 KTNPLTKSMR YYSLVNQSDE AGYLVEKTNH DTIGMVVRDT ENIFSAGTSS NGARFKIPGR 250 260 270 280 290 300 VGDSPIPGAG AYANKFGGAA ATGDGDVMMR FLPSFFAVTQ MELGTKPSKA AYKAITRILK 310 320 330 340 350 360 VFPKFSGAVV AMNVKGRIGA SCANINKFGY NVAFQNGTVV TYSISCLKEV NSLKYLKEGI EFA

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U50198 Genomic DNA. Translation: AAA91260.2.
PIR	T30965.
RefSeq	NP_508448.1.
UniGene	Cel.593
3D structure databases
HSSP	HSSP built from PDB template 1APY based on UniProtKB P20933.
ModBase	Search...
Protein family/group databases
MEROPS	T02.001.
Genome annotation databases
Ensembl	R04B3.2. Caenorhabditis elegans. [Contig view]
GeneID	180550.
KEGG	cel:R04B3.2.
NMPDR	fig|6239.3.peg.22677.
Organism-specific databases
WormBase	WBGene00019867. R04B3.2.
WormPep	R04B3.2. CE28745. [WorfDB]
Phylogenomic databases
OMA	Q21697. PLVLNTW.
Enzyme and pathway databases
BRENDA	3.5.1.26. 672.
Gene expression databases
ArrayExpress	Q21697.
Family and domain databases
InterPro	IPR000246. Peptidase_T2. [Graphical view]
PANTHER	PTHR10188. Peptidase_T2. 1 hit.
Pfam	PF01112. Asparaginase_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	909836.

Entry information

Entry name

ASPG_CAEEL

Accession

Primary (citable) accession number: Q21697

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	June 6, 2002
Last modified:	June 16, 2009
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project