Q215Z3 (Q215Z3_RHOPB) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Ordered Locus Names:

RPC_2239

Organism

Rhodopseudomonas palustris (strain BisB18) [Complete proteome] [HAMAP]

Taxonomic identifier

316056 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	922 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 RuleBase RU000518 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q215Z3 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: 18FD79B2F9BAC6F1	FASTA	922	100,208
	10 20 30 40 50 60 MNIVIVESPA KAKTINKYLG SSYEVLASFG HVRDLPAKNG SVDPDANFQM IWEIDPKAAG 70 80 90 100 110 120 RLNDIAKALK GADKLILATD PDREGEAISW HVLEVLKEKR AIKDHKIERV VFNAITKQAV 130 140 150 160 170 180 TDAMKNPRQI DGALVDAYMA RRALDYLVGF TLSPVLWRKL PGARSAGRVQ SVALRLVCDR 190 200 210 220 230 240 ELEIEKFVPR EYWSLVATLT TPRGEMFEAR LTGADGKKIQ RLDIGTGAEA EDFKQAIEAA 250 260 270 280 290 300 LFNVASVEAK PARRNPYAPF TTSTLQQEAS RKLGFAPAHT MRIAQRLYEG IDIGGETTGL 310 320 330 340 350 360 ITYMRTDGVQ IDSSAITQAR QVIGEDYGKQ YVPEAPRQYT AKAKNAQEAH EAIRPTDLSR 370 380 390 400 410 420 RPASLRARLD HDQIRLYELI WIRTIASQME SAELERTTVE IAAKAGSRVL ELRATGQVVK 430 440 450 460 470 480 FDGFLAVYQE GRDDDGDDED SRRLPAMSQG EALARKDLAV TQHFTEPPPR FSEASLVKRM 490 500 510 520 530 540 EELGIGRPST YASILQVLKD RGYVKLDKKR LHAEDKGRVV VAFLENFFAR YVEYDFTAAL 550 560 570 580 590 600 EENLDRISNN EISWQQVLRD FWTDFIGAVN DIKDLRVAQV LDALDDMLGS HIYAPRDDGG 610 620 630 640 650 660 DPRQCPSCGT GKLNLKAGKF GAFVGCSNYP ECRYTRPLAA DGGGDGDRIL GKDPVSGLEV 670 680 690 700 710 720 AVKAGRFGPY IQLGDAKDYA EGEKPKRAGI PKNSSPGDME LELALKLLSL PREVGKHPET 730 740 750 760 770 780 GEPIKAGIGR FGPYVQHEKT YASLEAGDEV FDIGLNRAVT LIAEKILKGP SKRRFGSDPG 790 800 810 820 830 840 KPLGEHPSLG TVAVKSGRYG AYVTAGGVNA TIPSDKTQES ITLPEAIALI DERAAKGGGK 850 860 870 880 890 900 PKKAAKKAPA KKAAKSDTDA AAETKKPAKK AAAKKSVAKP KADGVAVSAA RAPAKAKSST 910 920 KTAAAKKPAK PAAKKSAGKA NG

References

[1]

"Complete sequence of Rhodopseudomonas palustris BisB18." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Pelletier D.A., Kyrpides N., Anderson I., Oda Y., Harwood C.S., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BisB18.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000301 Genomic DNA. Translation: ABD87793.1.
RefSeq	YP_532112.1. NC_007925.1.
3D structure databases
ProteinModelPortal	Q215Z3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q215Z3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3973256.
GenomeReviews	Gene locus RPC_2239 in contig CP000301_GR.
KEGG	rpc:RPC_2239.
PATRIC	23269198. VBIRhoPal29154_2298.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0550.
HOGENOM	HBG512935.
OMA	KKVMPRL.
PhylomeDB	Q215Z3.
ProtClustDB	PRK07561.
Enzyme and pathway databases
BioCyc	RPAL316056:RPC_2239-MONOMER.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q215Z3_RHOPB

Accession

Primary (citable) accession number: Q215Z3

Entry history

Integrated into UniProtKB/TrEMBL:	April 18, 2006
Last sequence update:	April 18, 2006
Last modified:	December 14, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)