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Protein

Lipid-A-disaccharide synthase

Gene

lpxB

Organism
Rhodopseudomonas palustris (strain BisB18)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.UniRule annotation

Catalytic activityi

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate = UDP + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate.UniRule annotation

Pathway: lipid IV(A) biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (RPC_2447)
  2. UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciRPAL316056:GH3E-2478-MONOMER.
UniPathwayiUPA00359; UER00481.

Protein family/group databases

CAZyiGT19. Glycosyltransferase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid-A-disaccharide synthaseUniRule annotation (EC:2.4.1.182UniRule annotation)
Gene namesi
Name:lpxBUniRule annotation
Ordered Locus Names:RPC_2449
OrganismiRhodopseudomonas palustris (strain BisB18)
Taxonomic identifieri316056 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
ProteomesiUP000001948 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 396396Lipid-A-disaccharide synthasePRO_0000255215Add
BLAST

Proteomic databases

PRIDEiQ215D6.

Interactioni

Protein-protein interaction databases

STRINGi316056.RPC_2449.

Structurei

3D structure databases

ProteinModelPortaliQ215D6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0763.
HOGENOMiHOG000018004.
KOiK00748.
OMAiAPQEWAW.
OrthoDBiEOG6FBWZR.

Family and domain databases

HAMAPiMF_00392. LpxB.
InterProiIPR003835. Glyco_trans_19.
[Graphical view]
PfamiPF02684. LpxB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00215. lpxB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q215D6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAVATHAVR KIFLIATEES GDRLGASLMR ELRDRLGAAV RFEGVGGRAM
60 70 80 90 100
AREGLTSLFP IEELSIIGLS AVARRLPTIL RHIRTAAHAA LQAAPDVLVI
110 120 130 140 150
IDSPDFTHRV ARRVRARDPS IPIVNYVSPT VWAWRPGRAK VMRKYVDHVL
160 170 180 190 200
ALLPFEPDEY RRLQGPPCSY VGHPLTEQIA TLRPNPEEQL RRDAAPPVLL
210 220 230 240 250
VLPGSRRSEI RHHMAVFGEA LGLLQAQGVA FELILPTMPH LEALIAEALK
260 270 280 290 300
HWPLQPRVVV GENDKRAAFR IARAALAKSG TVTLELAVAG VPMVTAYRAG
310 320 330 340 350
QLEAWIVRRR ITSASVILAN LVVGENVAPE YLQEECTAPT LAAALRDVLA
360 370 380 390
DSPLRQRQLA AFGRIDAIMS TGAQSPSACA ADIVLGLLPA AAPALR
Length:396
Mass (Da):43,002
Last modified:April 18, 2006 - v1
Checksum:i1301C262E03031FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000301 Genomic DNA. Translation: ABD88000.1.
RefSeqiWP_011472897.1. NC_007925.1.
YP_532319.1. NC_007925.1.

Genome annotation databases

EnsemblBacteriaiABD88000; ABD88000; RPC_2449.
KEGGirpc:RPC_2449.
PATRICi23269634. VBIRhoPal29154_2513.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000301 Genomic DNA. Translation: ABD88000.1.
RefSeqiWP_011472897.1. NC_007925.1.
YP_532319.1. NC_007925.1.

3D structure databases

ProteinModelPortaliQ215D6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi316056.RPC_2449.

Protein family/group databases

CAZyiGT19. Glycosyltransferase Family 19.

Proteomic databases

PRIDEiQ215D6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD88000; ABD88000; RPC_2449.
KEGGirpc:RPC_2449.
PATRICi23269634. VBIRhoPal29154_2513.

Phylogenomic databases

eggNOGiCOG0763.
HOGENOMiHOG000018004.
KOiK00748.
OMAiAPQEWAW.
OrthoDBiEOG6FBWZR.

Enzyme and pathway databases

UniPathwayiUPA00359; UER00481.
BioCyciRPAL316056:GH3E-2478-MONOMER.

Family and domain databases

HAMAPiMF_00392. LpxB.
InterProiIPR003835. Glyco_trans_19.
[Graphical view]
PfamiPF02684. LpxB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00215. lpxB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BisB18.

Entry informationi

Entry nameiLPXB_RHOPB
AccessioniPrimary (citable) accession number: Q215D6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: April 18, 2006
Last modified: April 29, 2015
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.