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Q214H7 (GLYA_RHOPB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Serine hydroxymethyltransferase
Short name=SHMT
Short name=Serine methylase
EC=2.1.2.1
Gene names
Name:glyA
Ordered Locus Names:RPC_2659
OrganismRhodopseudomonas palustris (strain BisB18) [Complete proteome] [HAMAP]
Taxonomic identifier316056 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas ›

Protein attributes

Sequence length440 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism By similarity. HAMAP-Rule MF_00051

Catalytic activity

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP-Rule MF_00051

Cofactor

Pyridoxal phosphate By similarity.

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_00051

Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1. HAMAP-Rule MF_00051

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the SHMT family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
One-carbon metabolism
   Cellular componentCytoplasm
   LigandPyridoxal phosphate
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglycine biosynthetic process from serine

Inferred from electronic annotation. Source: HAMAP

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionglycine hydroxymethyltransferase activity

Inferred from electronic annotation. Source: HAMAP

pyridoxal phosphate binding

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 440440Serine hydroxymethyltransferase HAMAP-Rule MF_00051
PRO_0000369950

Regions

Region143 – 1453Substrate binding By similarity

Sites

Binding site531Pyridoxal phosphate By similarity
Binding site731Pyridoxal phosphate By similarity
Binding site751Substrate By similarity
Binding site821Substrate By similarity
Binding site831Pyridoxal phosphate By similarity
Binding site1171Pyridoxal phosphate By similarity
Binding site1391Substrate; via carbonyl oxygen By similarity
Binding site1941Pyridoxal phosphate By similarity
Binding site2221Pyridoxal phosphate By similarity
Binding site2471Pyridoxal phosphate By similarity
Binding site2541Pyridoxal phosphate By similarity
Binding site2801Pyridoxal phosphate; via amide nitrogen and carbonyl oxygen By similarity
Binding site3801Pyridoxal phosphate By similarity

Amino acid modifications

Modified residue2481N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q214H7 [UniParc].

Last modified April 18, 2006. Version 1.
Checksum: BB20FE437406547E

FASTA44046,646
        10         20         30         40         50         60 
MSASAKPAAK SAATASAPDS FFSATIADAD PEIAAAIAGE LGRQRHEIEL IASENIVSRA 

        70         80         90        100        110        120 
VMEAQGSVMT NKYAEGYPGH RYYGGCEFVD VAENLAIERA KKLFGAGFAN VQPNSGSQMN 

       130        140        150        160        170        180 
QAVFLALLQP GDTFMGLDLA AGGHLTHGSS VNMSGKWFKP VHYGVRREDG IIDMDEVAKI 

       190        200        210        220        230        240 
AEANKPKLII AGGSAYSRAW DFKRFREIAD SVGAYFMVDM AHFAGLVAGG VHANPVPHAH 

       250        260        270        280        290        300 
VVTTTTHKSL RGPRGGLILT NDEALAKKFN SAIFPGLQGG PLMHVIAAKA VAFKEALQPD 

       310        320        330        340        350        360 
FKVYAKNVVE NAKALAETLR GHGFDIVSGG TDNHLMLVDL RPKSLKGNVS EKALVRAGIT 

       370        380        390        400        410        420 
CNKNGIPYDP EKPFVTSGIR LGTPAATTRG FGVAEFQQVG GMIAEVLNAI AQSEDGTAPL 

       430        440 
VEAAVKARVK ELTDRFPIYQ

« Hide

References

[1]"Complete sequence of Rhodopseudomonas palustris BisB18."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Pelletier D.A., Kyrpides N., Anderson I., Oda Y., Harwood C.S., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BisB18.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000301 Genomic DNA. Translation: ABD88209.1.
RefSeqYP_532528.1. NC_007925.1.

3D structure databases

ProteinModelPortalQ214H7.
SMRQ214H7. Positions 30-434.
ModBaseSearch...

Protein-protein interaction databases

STRING316056.RPC_2659.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD88209; ABD88209; RPC_2659.
GeneID3970519.
KEGGrpc:RPC_2659.
PATRIC23270080. VBIRhoPal29154_2732.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0112.
HOGENOMHOG000239404.
KOK00600.
OMAFKAYAHQ.
ProtClustDBPRK00011.

Enzyme and pathway databases

BioCycRPAL316056:GH3E-2732-MONOMER.
UniPathwayUPA00193.
UPA00288; UER01023.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPMF_00051. SHMT.
InterProIPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
PANTHERPTHR11680. PTHR11680. 1 hit.
PfamPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFPIRSF000412. SHMT. 1 hit.
SUPFAMSSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITEPS00096. SHMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLYA_RHOPB
AccessionPrimary (citable) accession number: Q214H7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 18, 2006
Last modified: May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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