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Q21452

- LIAS_CAEEL

UniProt

Q21452 - LIAS_CAEEL

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Protein

Lipoyl synthase, mitochondrial

Gene

M01F1.3

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi91 – 911Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi96 – 961Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi102 – 1021Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi122 – 1221Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi126 – 1261Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi129 – 1291Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
ORF Names:M01F1.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome III

Organism-specific databases

WormBaseiM01F1.3; CE01031; WBGene00010809.

Subcellular locationi

Mitochondrion UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 354Lipoyl synthase, mitochondrialPRO_0000398228
Transit peptidei1 – ?MitochondrionUniRule annotation

Proteomic databases

PaxDbiQ21452.

Interactioni

Protein-protein interaction databases

BioGridi532131. 7 interactions.
STRINGi6239.M01F1.3.3.

Structurei

3D structure databases

ProteinModelPortaliQ21452.
SMRiQ21452. Positions 58-342.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
GeneTreeiENSGT00390000006234.
HOGENOMiHOG000235998.
InParanoidiQ21452.
KOiK03644.
OMAiTIRAVRH.
PhylomeDBiQ21452.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q21452-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLARVWVRGL AATKKKPQVL VKDGPSLQDF ISSASVAEAV EKYEGKLKLE
60 70 80 90 100
KGDRRLRLPP WLKKEKILPS ENENVSRLKK QLKHLKLATV CQEARCPNLG
110 120 130 140 150
ECWGGSDDSL ATATIMLMGD TCTRGCKFCS VKTARAPPPL DPMEPENTST
160 170 180 190 200
AVASWGVEYI VLTSVDRDDL PDGGADHLRK TVQLMKLKKP ELLIECLLPD
210 220 230 240 250
FAGDKISVEK MATSGLDVYA HNIETVERLT PWVRDPRAKY RQSLDALRYA
260 270 280 290 300
KEVSPKLITK TSIMLGLGEA EDEIKQCLAD LRASNVDVVT FGQYMQPTKR
310 320 330 340 350
HLLVKEWVTP EKFDQWAEYS KKLGFLYVAS GPLVRSSYKA GEFYLKNVLR

NRQN
Length:354
Mass (Da):39,767
Last modified:November 1, 1996 - v1
Checksum:i5428CFA42A7FCDEE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46381 Genomic DNA. Translation: CAA86516.1.
PIRiT23655.
RefSeqiNP_497722.3. NM_065321.6.
UniGeneiCel.38595.

Genome annotation databases

EnsemblMetazoaiM01F1.3.1; M01F1.3.1; WBGene00010809.
M01F1.3.2; M01F1.3.2; WBGene00010809.
M01F1.3.3; M01F1.3.3; WBGene00010809.
GeneIDi3564813.
KEGGicel:CELE_M01F1.3.
UCSCiM01F1.3.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46381 Genomic DNA. Translation: CAA86516.1 .
PIRi T23655.
RefSeqi NP_497722.3. NM_065321.6.
UniGenei Cel.38595.

3D structure databases

ProteinModelPortali Q21452.
SMRi Q21452. Positions 58-342.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 532131. 7 interactions.
STRINGi 6239.M01F1.3.3.

Proteomic databases

PaxDbi Q21452.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai M01F1.3.1 ; M01F1.3.1 ; WBGene00010809 .
M01F1.3.2 ; M01F1.3.2 ; WBGene00010809 .
M01F1.3.3 ; M01F1.3.3 ; WBGene00010809 .
GeneIDi 3564813.
KEGGi cel:CELE_M01F1.3.
UCSCi M01F1.3.1. c. elegans.

Organism-specific databases

CTDi 3564813.
WormBasei M01F1.3 ; CE01031 ; WBGene00010809 .

Phylogenomic databases

eggNOGi COG0320.
GeneTreei ENSGT00390000006234.
HOGENOMi HOG000235998.
InParanoidi Q21452.
KOi K03644.
OMAi TIRAVRH.
PhylomeDBi Q21452.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Miscellaneous databases

NextBioi 954083.
PROi Q21452.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiLIAS_CAEEL
AccessioniPrimary (citable) accession number: Q21452
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3