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Q21452

- LIAS_CAEEL

UniProt

Q21452 - LIAS_CAEEL

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Protein
Lipoyl synthase, mitochondrial
Gene
M01F1.3
Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi91 – 911Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi96 – 961Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi102 – 1021Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi122 – 1221Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi126 – 1261Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi129 – 1291Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrial (EC:2.8.1.8)
Alternative name(s):
Lipoate synthase
Short name:
LS
Short name:
Lip-syn
Lipoic acid synthase
Gene namesi
ORF Names:M01F1.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome III

Organism-specific databases

WormBaseiM01F1.3; CE01031; WBGene00010809.

Subcellular locationi

Mitochondrion By similarity UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 354Lipoyl synthase, mitochondrialUniRule annotationPRO_0000398228
Transit peptidei1 – ?Mitochondrion Reviewed prediction

Proteomic databases

PaxDbiQ21452.

Interactioni

Protein-protein interaction databases

BioGridi532131. 7 interactions.
STRINGi6239.M01F1.3.3.

Structurei

3D structure databases

ProteinModelPortaliQ21452.
SMRiQ21452. Positions 116-295.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
GeneTreeiENSGT00390000006234.
HOGENOMiHOG000235998.
InParanoidiQ21452.
KOiK03644.
OMAiTIRAVRH.
PhylomeDBiQ21452.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q21452-1 [UniParc]FASTAAdd to Basket

« Hide

MLARVWVRGL AATKKKPQVL VKDGPSLQDF ISSASVAEAV EKYEGKLKLE    50
KGDRRLRLPP WLKKEKILPS ENENVSRLKK QLKHLKLATV CQEARCPNLG 100
ECWGGSDDSL ATATIMLMGD TCTRGCKFCS VKTARAPPPL DPMEPENTST 150
AVASWGVEYI VLTSVDRDDL PDGGADHLRK TVQLMKLKKP ELLIECLLPD 200
FAGDKISVEK MATSGLDVYA HNIETVERLT PWVRDPRAKY RQSLDALRYA 250
KEVSPKLITK TSIMLGLGEA EDEIKQCLAD LRASNVDVVT FGQYMQPTKR 300
HLLVKEWVTP EKFDQWAEYS KKLGFLYVAS GPLVRSSYKA GEFYLKNVLR 350
NRQN 354
Length:354
Mass (Da):39,767
Last modified:November 1, 1996 - v1
Checksum:i5428CFA42A7FCDEE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46381 Genomic DNA. Translation: CAA86516.1.
PIRiT23655.
RefSeqiNP_497722.3. NM_065321.6.
UniGeneiCel.38595.

Genome annotation databases

EnsemblMetazoaiM01F1.3.1; M01F1.3.1; WBGene00010809.
M01F1.3.2; M01F1.3.2; WBGene00010809.
M01F1.3.3; M01F1.3.3; WBGene00010809.
GeneIDi3564813.
KEGGicel:CELE_M01F1.3.
UCSCiM01F1.3.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46381 Genomic DNA. Translation: CAA86516.1 .
PIRi T23655.
RefSeqi NP_497722.3. NM_065321.6.
UniGenei Cel.38595.

3D structure databases

ProteinModelPortali Q21452.
SMRi Q21452. Positions 116-295.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 532131. 7 interactions.
STRINGi 6239.M01F1.3.3.

Proteomic databases

PaxDbi Q21452.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai M01F1.3.1 ; M01F1.3.1 ; WBGene00010809 .
M01F1.3.2 ; M01F1.3.2 ; WBGene00010809 .
M01F1.3.3 ; M01F1.3.3 ; WBGene00010809 .
GeneIDi 3564813.
KEGGi cel:CELE_M01F1.3.
UCSCi M01F1.3.1. c. elegans.

Organism-specific databases

CTDi 3564813.
WormBasei M01F1.3 ; CE01031 ; WBGene00010809 .

Phylogenomic databases

eggNOGi COG0320.
GeneTreei ENSGT00390000006234.
HOGENOMi HOG000235998.
InParanoidi Q21452.
KOi K03644.
OMAi TIRAVRH.
PhylomeDBi Q21452.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Miscellaneous databases

NextBioi 954083.
PROi Q21452.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiLIAS_CAEEL
AccessioniPrimary (citable) accession number: Q21452
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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