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Q21452

- LIAS_CAEEL

UniProt

Q21452 - LIAS_CAEEL

Protein

Lipoyl synthase, mitochondrial

Gene

M01F1.3

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi91 – 911Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi96 – 961Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi102 – 1021Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi122 – 1221Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi126 – 1261Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi129 – 1291Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Short name:
    LSUniRule annotation
    Short name:
    Lip-synUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    ORF Names:M01F1.3
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome III

    Organism-specific databases

    WormBaseiM01F1.3; CE01031; WBGene00010809.

    Subcellular locationi

    Mitochondrion UniRule annotation

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 354Lipoyl synthase, mitochondrialPRO_0000398228
    Transit peptidei1 – ?MitochondrionUniRule annotation

    Proteomic databases

    PaxDbiQ21452.

    Interactioni

    Protein-protein interaction databases

    BioGridi532131. 7 interactions.
    STRINGi6239.M01F1.3.3.

    Structurei

    3D structure databases

    ProteinModelPortaliQ21452.
    SMRiQ21452. Positions 116-295.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0320.
    GeneTreeiENSGT00390000006234.
    HOGENOMiHOG000235998.
    InParanoidiQ21452.
    KOiK03644.
    OMAiTIRAVRH.
    PhylomeDBiQ21452.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q21452-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLARVWVRGL AATKKKPQVL VKDGPSLQDF ISSASVAEAV EKYEGKLKLE    50
    KGDRRLRLPP WLKKEKILPS ENENVSRLKK QLKHLKLATV CQEARCPNLG 100
    ECWGGSDDSL ATATIMLMGD TCTRGCKFCS VKTARAPPPL DPMEPENTST 150
    AVASWGVEYI VLTSVDRDDL PDGGADHLRK TVQLMKLKKP ELLIECLLPD 200
    FAGDKISVEK MATSGLDVYA HNIETVERLT PWVRDPRAKY RQSLDALRYA 250
    KEVSPKLITK TSIMLGLGEA EDEIKQCLAD LRASNVDVVT FGQYMQPTKR 300
    HLLVKEWVTP EKFDQWAEYS KKLGFLYVAS GPLVRSSYKA GEFYLKNVLR 350
    NRQN 354
    Length:354
    Mass (Da):39,767
    Last modified:November 1, 1996 - v1
    Checksum:i5428CFA42A7FCDEE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46381 Genomic DNA. Translation: CAA86516.1.
    PIRiT23655.
    RefSeqiNP_497722.3. NM_065321.6.
    UniGeneiCel.38595.

    Genome annotation databases

    EnsemblMetazoaiM01F1.3.1; M01F1.3.1; WBGene00010809.
    M01F1.3.2; M01F1.3.2; WBGene00010809.
    M01F1.3.3; M01F1.3.3; WBGene00010809.
    GeneIDi3564813.
    KEGGicel:CELE_M01F1.3.
    UCSCiM01F1.3.1. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46381 Genomic DNA. Translation: CAA86516.1 .
    PIRi T23655.
    RefSeqi NP_497722.3. NM_065321.6.
    UniGenei Cel.38595.

    3D structure databases

    ProteinModelPortali Q21452.
    SMRi Q21452. Positions 116-295.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 532131. 7 interactions.
    STRINGi 6239.M01F1.3.3.

    Proteomic databases

    PaxDbi Q21452.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai M01F1.3.1 ; M01F1.3.1 ; WBGene00010809 .
    M01F1.3.2 ; M01F1.3.2 ; WBGene00010809 .
    M01F1.3.3 ; M01F1.3.3 ; WBGene00010809 .
    GeneIDi 3564813.
    KEGGi cel:CELE_M01F1.3.
    UCSCi M01F1.3.1. c. elegans.

    Organism-specific databases

    CTDi 3564813.
    WormBasei M01F1.3 ; CE01031 ; WBGene00010809 .

    Phylogenomic databases

    eggNOGi COG0320.
    GeneTreei ENSGT00390000006234.
    HOGENOMi HOG000235998.
    InParanoidi Q21452.
    KOi K03644.
    OMAi TIRAVRH.
    PhylomeDBi Q21452.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Miscellaneous databases

    NextBioi 954083.
    PROi Q21452.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.

    Entry informationi

    Entry nameiLIAS_CAEEL
    AccessioniPrimary (citable) accession number: Q21452
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3