Reviewed, UniProtKB/Swiss-Prot Q21432 (NAS11_CAEEL)

Names and origin

Protein names

Recommended name:
    Zinc metalloproteinase nas-11
    EC=3.4.24.21
Alternative name(s):
    Nematode astacin 11

Gene names

Name:	nas-11
ORF Names:	K11G12.1

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	579 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Essential metalloprotease.
Catalytic activity	Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Secreted Potential.
Disruption phenotype	Defects lead to retarded growth. Ref.2
Sequence similarities	Belongs to the peptidase M12A family. Contains 1 SXC (ShKT) domain.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: Q21432-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform b (identifier: Q21432-2)

The sequence of this isoform differs from the canonical sequence as follows:
     400-455: FCGLSYVGRA...DRDQFITINW → LTKELQFMKQ...WIQNCTLHTE
     456-579: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 579	562	Zinc metalloproteinase nas-11		PRO_0000028915
Regions
	Domain	539 – 576	38	SXC
	Compositional bias	301 – 311	11	Poly-Asp
Sites
	Active site	431	1	By similarity
	Metal binding	430	1	Zinc; catalytic By similarity
	Metal binding	434	1	Zinc; catalytic By similarity
	Metal binding	440	1	Zinc; catalytic By similarity
Amino acid modifications
	Glycosylation	256	1	N-linked (GlcNAc...) Ref.3
	Glycosylation	454	1	N-linked (GlcNAc...) Potential
	Disulfide bond	539 ↔ 575		By similarity
	Disulfide bond	546 ↔ 568		By similarity
	Disulfide bond	555 ↔ 572		By similarity
Natural variations
	Alternative sequence	400 – 455	56	FCGLS…ITINW → LTKELQFMKQCMRSELPIST FAMIVINLLLLIGVTLIHNN TMHLLWWIQNCTLHTE in isoform b.		VSP_012354
	Alternative sequence	456 – 579	124	Missing in isoform b.		VSP_012355

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified March 1, 2004. Version 2. Checksum: 2E98DC8E8AAAB744	FASTA	579	65,031
	10 20 30 40 50 60 MTPSLVFLIV VIVVVEGQGW RPWDRFNHPG NFGNWGGNNW GTRQRNQEPH DIPPPVPPPG 70 80 90 100 110 120 FRGNNDRFGG NIIKVVEIID LGKSKNRGDI LSDFKDVHKK HRHLGRKEWK GKVKQFCHRF 130 140 150 160 170 180 PGHPNCRRGK VPDQKELEEM IGQFSKGGIG RFLKRVPKIY IEDPLARVDP KLKGFLENAG 190 200 210 220 230 240 RGFGHVSSEH VNKLRDICKR RKCREQPESA KKTRELFTQK LADFETKIAG KDKTDSVQLR 250 260 270 280 290 300 FDRTLQIKEA LLEKGNLTAD IVPVDNGVYD LDTMLTEEQA NILLNELNKA GVGDDEIPLP 310 320 330 340 350 360 DADTDDEDDD DSTNSASGAA PGSSRLKKSA LYFEGNLIKK WDPSSPIRYV LDSSLEDLDK 370 380 390 400 410 420 NDVRAAIYEI EKNTCIRFKE LSSPPTGSHI VYYKVDSPTF CGLSYVGRAD PANPVYLSFG 430 440 450 460 470 480 CDNNKGVAIH ETMHALGVAH QHLRNDRDQF ITINWSNIDP QQYDAFVVVD SKLYTSYGVK 490 500 510 520 530 540 YAYDSIMHYN GYTAAQNIAI PTMNPKTNSA VNLKVLGQRQ KMGTTDIELL KKMYCQPGCD 550 560 570 DKNVYCGAWA LKDLCKNPGH DQYMAANCKK SCGLCAIGK
	Isoform b. Checksum: 4100A3CA90772C71	FASTA	455	51,639
	10 20 30 40 50 60 MTPSLVFLIV VIVVVEGQGW RPWDRFNHPG NFGNWGGNNW GTRQRNQEPH DIPPPVPPPG 70 80 90 100 110 120 FRGNNDRFGG NIIKVVEIID LGKSKNRGDI LSDFKDVHKK HRHLGRKEWK GKVKQFCHRF 130 140 150 160 170 180 PGHPNCRRGK VPDQKELEEM IGQFSKGGIG RFLKRVPKIY IEDPLARVDP KLKGFLENAG 190 200 210 220 230 240 RGFGHVSSEH VNKLRDICKR RKCREQPESA KKTRELFTQK LADFETKIAG KDKTDSVQLR 250 260 270 280 290 300 FDRTLQIKEA LLEKGNLTAD IVPVDNGVYD LDTMLTEEQA NILLNELNKA GVGDDEIPLP 310 320 330 340 350 360 DADTDDEDDD DSTNSASGAA PGSSRLKKSA LYFEGNLIKK WDPSSPIRYV LDSSLEDLDK 370 380 390 400 410 420 NDVRAAIYEI EKNTCIRFKE LSSPPTGSHI VYYKVDSPTL TKELQFMKQC MRSELPISTF 430 440 450 AMIVINLLLL IGVTLIHNNT MHLLWWIQNC TLHTE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
[2]	"The astacin protein family in Caenorhabditis elegans." Moehrlen F., Hutter H., Zwilling R. Eur. J. Biochem. 270:4909-4920(2003) [PubMed: 14653817] [Abstract] Cited for: NOMENCLATURE, DISRUPTION PHENOTYPE.
[3]	"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins." Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T. Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed: 17761667] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-256, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U23525 Genomic DNA. Translation: AAC46566.2. U23525 Genomic DNA. Translation: AAU05558.1.
PIR	T16617.
RefSeq	NP_001024789.1.
UniGene	Cel.17648
3D structure databases
HSSP	HSSP built from PDB template 1IAE based on UniProtKB P07584.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:24488N.
IntAct	Q21432. 1 interaction.
Protein family/group databases
MEROPS	M12.323.
Proteomic databases
PRIDE	Q21432.
Genome annotation databases
Ensembl	K11G12.1. Caenorhabditis elegans. [Contig view]
GeneID	180938.
KEGG	cel:K11G12.1.
NMPDR	fig|6239.3.peg.23608.
Organism-specific databases
WormBase	WBGene00003530. nas-11.
WormPep	K11G12.1a. CE36313. [WorfDB] K11G12.1b. CE37260. [WorfDB]
Phylogenomic databases
OMA	Q21432. REQPESA.
Enzyme and pathway databases
BRENDA	3.4.24.21. 672.
Gene expression databases
ArrayExpress	Q21432.
Family and domain databases
InterPro	IPR006025. Pept_M_Zn_BS. IPR006026. Peptidase_M. IPR001506. Peptidase_M12A. IPR017368. Peptidase_M12A_astacin-9/10/11. IPR003582. ShK_toxin. [Graphical view]
Pfam	PF01400. Astacin. 1 hit. PF01549. ShK. 1 hit. [Graphical view]
PIRSF	PIRSF038055. Nas9/Nas10/Nas11. 1 hit.
PRINTS	PR00480. ASTACIN.
SMART	SM00254. ShKT. 1 hit. SM00235. ZnMc. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	911646.

Entry information

Entry name

NAS11_CAEEL

Accession

Primary (citable) accession number: Q21432
Secondary accession number(s): Q65ZJ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	March 1, 2004
Last modified:	June 16, 2009
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project