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Protein

Zinc metalloproteinase nas-11

Gene

nas-11

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential metalloprotease.

Catalytic activityi

Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi430 – 4301Zinc; catalyticBy similarity
Active sitei431 – 4311By similarity
Metal bindingi434 – 4341Zinc; catalyticBy similarity
Metal bindingi440 – 4401Zinc; catalyticBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM12.A36.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc metalloproteinase nas-11 (EC:3.4.24.21)
Alternative name(s):
Nematode astacin 11
Gene namesi
Name:nas-11
ORF Names:K11G12.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiK11G12.1a; CE36313; WBGene00003530; nas-11.
K11G12.1b; CE37260; WBGene00003530; nas-11.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Defects lead to retarded growth.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence analysisAdd
BLAST
Chaini18 – 579562Zinc metalloproteinase nas-11PRO_0000028915Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi256 – 2561N-linked (GlcNAc...)1 Publication
Glycosylationi454 – 4541N-linked (GlcNAc...)Sequence analysis
Disulfide bondi539 ↔ 575PROSITE-ProRule annotation
Disulfide bondi546 ↔ 568PROSITE-ProRule annotation
Disulfide bondi555 ↔ 572PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ21432.
PaxDbiQ21432.
PRIDEiQ21432.

Expressioni

Gene expression databases

BgeeiWBGene00003530.

Interactioni

Protein-protein interaction databases

BioGridi45867. 1 interaction.
DIPiDIP-24488N.
MINTiMINT-1048417.
STRINGi6239.K11G12.1a.

Structurei

3D structure databases

ProteinModelPortaliQ21432.
SMRiQ21432. Positions 359-534.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini539 – 57537ShKTPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi301 – 31111Poly-AspAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase M12A family.Curated
Contains 1 ShKT domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3714. Eukaryota.
ENOG410ZPX7. LUCA.
GeneTreeiENSGT00760000119227.
HOGENOMiHOG000018720.
InParanoidiQ21432.
OMAiFITINWS.
OrthoDBiEOG091G05O5.
PhylomeDBiQ21432.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR001506. Peptidase_M12A.
IPR017368. Peptidase_M12A_astacin-9/10/11.
IPR006026. Peptidase_Metallo.
IPR003582. ShKT_dom.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
PF01549. ShK. 1 hit.
[Graphical view]
PIRSFiPIRSF038055. Nas9/Nas10/Nas11. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00254. ShKT. 1 hit.
SM00235. ZnMc. 1 hit.
[Graphical view]
PROSITEiPS51670. SHKT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q21432-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTPSLVFLIV VIVVVEGQGW RPWDRFNHPG NFGNWGGNNW GTRQRNQEPH
60 70 80 90 100
DIPPPVPPPG FRGNNDRFGG NIIKVVEIID LGKSKNRGDI LSDFKDVHKK
110 120 130 140 150
HRHLGRKEWK GKVKQFCHRF PGHPNCRRGK VPDQKELEEM IGQFSKGGIG
160 170 180 190 200
RFLKRVPKIY IEDPLARVDP KLKGFLENAG RGFGHVSSEH VNKLRDICKR
210 220 230 240 250
RKCREQPESA KKTRELFTQK LADFETKIAG KDKTDSVQLR FDRTLQIKEA
260 270 280 290 300
LLEKGNLTAD IVPVDNGVYD LDTMLTEEQA NILLNELNKA GVGDDEIPLP
310 320 330 340 350
DADTDDEDDD DSTNSASGAA PGSSRLKKSA LYFEGNLIKK WDPSSPIRYV
360 370 380 390 400
LDSSLEDLDK NDVRAAIYEI EKNTCIRFKE LSSPPTGSHI VYYKVDSPTF
410 420 430 440 450
CGLSYVGRAD PANPVYLSFG CDNNKGVAIH ETMHALGVAH QHLRNDRDQF
460 470 480 490 500
ITINWSNIDP QQYDAFVVVD SKLYTSYGVK YAYDSIMHYN GYTAAQNIAI
510 520 530 540 550
PTMNPKTNSA VNLKVLGQRQ KMGTTDIELL KKMYCQPGCD DKNVYCGAWA
560 570
LKDLCKNPGH DQYMAANCKK SCGLCAIGK
Note: No experimental confirmation available.
Length:579
Mass (Da):65,031
Last modified:March 1, 2004 - v2
Checksum:i2E98DC8E8AAAB744
GO
Isoform b (identifier: Q21432-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     400-455: FCGLSYVGRA...DRDQFITINW → LTKELQFMKQ...WIQNCTLHTE
     456-579: Missing.

Note: No experimental confirmation available.
Show »
Length:455
Mass (Da):51,639
Checksum:i4100A3CA90772C71
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei400 – 45556FCGLS…ITINW → LTKELQFMKQCMRSELPIST FAMIVINLLLLIGVTLIHNN TMHLLWWIQNCTLHTE in isoform b. CuratedVSP_012354Add
BLAST
Alternative sequencei456 – 579124Missing in isoform b. CuratedVSP_012355Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081325 Genomic DNA. Translation: CCD70792.1.
FO081325 Genomic DNA. Translation: CCD70793.1.
PIRiT16617.
RefSeqiNP_001024789.1. NM_001029618.4. [Q21432-1]
NP_001024790.1. NM_001029619.5. [Q21432-2]
UniGeneiCel.17648.

Genome annotation databases

EnsemblMetazoaiK11G12.1a; K11G12.1a; WBGene00003530. [Q21432-1]
GeneIDi180938.
KEGGicel:CELE_K11G12.1.
UCSCiK11G12.1b. c. elegans. [Q21432-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081325 Genomic DNA. Translation: CCD70792.1.
FO081325 Genomic DNA. Translation: CCD70793.1.
PIRiT16617.
RefSeqiNP_001024789.1. NM_001029618.4. [Q21432-1]
NP_001024790.1. NM_001029619.5. [Q21432-2]
UniGeneiCel.17648.

3D structure databases

ProteinModelPortaliQ21432.
SMRiQ21432. Positions 359-534.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi45867. 1 interaction.
DIPiDIP-24488N.
MINTiMINT-1048417.
STRINGi6239.K11G12.1a.

Protein family/group databases

MEROPSiM12.A36.

Proteomic databases

EPDiQ21432.
PaxDbiQ21432.
PRIDEiQ21432.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiK11G12.1a; K11G12.1a; WBGene00003530. [Q21432-1]
GeneIDi180938.
KEGGicel:CELE_K11G12.1.
UCSCiK11G12.1b. c. elegans. [Q21432-1]

Organism-specific databases

CTDi180938.
WormBaseiK11G12.1a; CE36313; WBGene00003530; nas-11.
K11G12.1b; CE37260; WBGene00003530; nas-11.

Phylogenomic databases

eggNOGiKOG3714. Eukaryota.
ENOG410ZPX7. LUCA.
GeneTreeiENSGT00760000119227.
HOGENOMiHOG000018720.
InParanoidiQ21432.
OMAiFITINWS.
OrthoDBiEOG091G05O5.
PhylomeDBiQ21432.

Miscellaneous databases

PROiQ21432.

Gene expression databases

BgeeiWBGene00003530.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR001506. Peptidase_M12A.
IPR017368. Peptidase_M12A_astacin-9/10/11.
IPR006026. Peptidase_Metallo.
IPR003582. ShKT_dom.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
PF01549. ShK. 1 hit.
[Graphical view]
PIRSFiPIRSF038055. Nas9/Nas10/Nas11. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00254. ShKT. 1 hit.
SM00235. ZnMc. 1 hit.
[Graphical view]
PROSITEiPS51670. SHKT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNAS11_CAEEL
AccessioniPrimary (citable) accession number: Q21432
Secondary accession number(s): Q65ZJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: March 1, 2004
Last modified: September 7, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.