ID   NAS10_CAEEL             Reviewed;         560 AA.
AC   Q21388;
DT   04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 53.
DE   RecName: Full=Zinc metalloproteinase nas-10;
DE            EC=3.4.24.21;
DE   AltName: Full=Nematode astacin 10;
GN   Name=nas-10; ORFNames=K09C8.3;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Nematoda; Chromadorea; Rhabditida; Rhabditoidea;
OC   Rhabditidae; Peloderinae; Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   MEDLINE=99069613; PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for
RT   investigating biology.";
RL   Science 282:2012-2018(1998).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=14653817; DOI=10.1046/j.1432-1033.2003.03891.x;
RA   Moehrlen F., Hutter H., Zwilling R.;
RT   "The astacin protein family in Caenorhabditis elegans.";
RL   Eur. J. Biochem. 270:4909-4920(2003).
CC   -!- FUNCTION: Probable metalloprotease (By similarity).
CC   -!- CATALYTIC ACTIVITY: Hydrolysis of peptide bonds in substrates
CC       containing five or more amino acids, preferentially with Ala in
CC       P1', and Pro in P2'.
CC   -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC   -!- SIMILARITY: Belongs to the peptidase M12A family.
CC   -!- SIMILARITY: Contains 1 SXC (ShKT) domain.
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DR   EMBL; Z68006; CAA91996.1; -; Genomic_DNA.
DR   PIR; T23540; T23540.
DR   RefSeq; NP_509832.1; -.
DR   UniGene; Cel.696; -.
DR   HSSP; P07584; 1IAE.
DR   MEROPS; M12.323; -.
DR   Ensembl; K09C8.3; Caenorhabditis elegans.
DR   GeneID; 181287; -.
DR   KEGG; cel:K09C8.3; -.
DR   NMPDR; fig|6239.3.peg.24545; -.
DR   WormBase; WBGene00003529; nas-10.
DR   WormPep; K09C8.3; CE03479.
DR   OMA; Q21388; GRVEPAN.
DR   BRENDA; 3.4.24.21; 672.
DR   NextBio; 913288; -.
DR   ArrayExpress; Q21388; -.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR   InterPro; IPR006025; Pept_M_Zn_BS.
DR   InterPro; IPR006026; Peptidase_M.
DR   InterPro; IPR001506; Peptidase_M12A.
DR   InterPro; IPR017368; Peptidase_M12A_astacin-9/10/11.
DR   InterPro; IPR003582; ShK_toxin.
DR   Pfam; PF01400; Astacin; 1.
DR   Pfam; PF01549; ShK; 1.
DR   PIRSF; PIRSF038055; Nas9/Nas10/Nas11; 1.
DR   PRINTS; PR00480; ASTACIN.
DR   SMART; SM00254; ShKT; 1.
DR   SMART; SM00235; ZnMc; 1.
DR   PROSITE; PS00142; ZINC_PROTEASE; FALSE_NEG.
PE   2: Evidence at transcript level;
KW   Complete proteome; Disulfide bond; Hydrolase; Metal-binding;
KW   Metalloprotease; Protease; Zinc.
FT   CHAIN         1    560       Zinc metalloproteinase nas-10.
FT                                /FTId=PRO_0000078185.
FT   DOMAIN      524    560       SXC.
FT   COMPBIAS    111    114       Poly-Pro.
FT   COMPBIAS    187    227       Gln-rich.
FT   ACT_SITE    415    415       By similarity.
FT   METAL       414    414       Zinc; catalytic (By similarity).
FT   METAL       418    418       Zinc; catalytic (By similarity).
FT   METAL       424    424       Zinc; catalytic (By similarity).
FT   DISULFID    524    560       By similarity.
FT   DISULFID    531    553       By similarity.
FT   DISULFID    540    557       By similarity.
SQ   SEQUENCE   560 AA;  63041 MW;  4490B5456DA7256A CRC64;
     MYTITDLKTI NRKQKQAYLQ EKLICSRHCW LTHTTSKHIN IQWTTLIPHI RCRGFNNGPH
     PNSRPGSRPN SPLGDIFGNI NGMVKGITDQ IGKIAQGLDV NNDLGKMAHG PPPPQSEWVE
     HARRFCRRFP GHPKCRGQLP QFNDIGSMLN GILVDSGKWL PKVPFINIRD PLSGINSDLK
     NALNGIQVQF GQISQQFANN IRNICQQMNC KQQQQKNVQM KQAILKQTVD FEKKVFGNNV
     ADKMNLRFDR TLQLKQALLE KAQLKGVVAP EDNGVFDKDL LLTETQANFM LNELGKGGEG
     AIPMPGSAKA KRASIFFEQN LIQKWPSTSP IPYTFDSSLD NLDQNDVRGA ISEIEQKTCI
     RFKYFASPPK GNHINYQKVN SPSFCGLSYI GRVEPANPVY LSFQCGNGRG IAVHETMHAL
     GVNHQHLRMD RDKHIKVDWS NINPQQYDAF VVADSKLYTT YGVKYAYDSI MHYNAYTGAV
     NIAKPTMIPL VNQQANIGLL GQRAKMSNAD VEILNKMYCK SAGCDDKNVY CGAWALQDLC
     NNPNHNVWMR SNCRKSCNFC
//