Reviewed, UniProtKB/Swiss-Prot Q21388 (NAS10_CAEEL)

Names and origin

Protein names

Recommended name:
    Zinc metalloproteinase nas-10
    EC=3.4.24.21
Alternative name(s):
    Nematode astacin 10

Gene names

Name:	nas-10
ORF Names:	K09C8.3

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probable metalloprotease By similarity.
Catalytic activity	Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Sequence similarities	Belongs to the peptidase M12A family. Contains 1 SXC (ShKT) domain.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 560	560	Zinc metalloproteinase nas-10		PRO_0000078185
Regions
	Domain	524 – 560	37	SXC
	Compositional bias	111 – 114	4	Poly-Pro
	Compositional bias	187 – 227	41	Gln-rich
Sites
	Active site	415	1	By similarity
	Metal binding	414	1	Zinc; catalytic By similarity
	Metal binding	418	1	Zinc; catalytic By similarity
	Metal binding	424	1	Zinc; catalytic By similarity
Amino acid modifications
	Disulfide bond	524 ↔ 560		By similarity
	Disulfide bond	531 ↔ 553		By similarity
	Disulfide bond	540 ↔ 557		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q21388-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4490B5456DA7256A	FASTA	560	63,041
	10 20 30 40 50 60 MYTITDLKTI NRKQKQAYLQ EKLICSRHCW LTHTTSKHIN IQWTTLIPHI RCRGFNNGPH 70 80 90 100 110 120 PNSRPGSRPN SPLGDIFGNI NGMVKGITDQ IGKIAQGLDV NNDLGKMAHG PPPPQSEWVE 130 140 150 160 170 180 HARRFCRRFP GHPKCRGQLP QFNDIGSMLN GILVDSGKWL PKVPFINIRD PLSGINSDLK 190 200 210 220 230 240 NALNGIQVQF GQISQQFANN IRNICQQMNC KQQQQKNVQM KQAILKQTVD FEKKVFGNNV 250 260 270 280 290 300 ADKMNLRFDR TLQLKQALLE KAQLKGVVAP EDNGVFDKDL LLTETQANFM LNELGKGGEG 310 320 330 340 350 360 AIPMPGSAKA KRASIFFEQN LIQKWPSTSP IPYTFDSSLD NLDQNDVRGA ISEIEQKTCI 370 380 390 400 410 420 RFKYFASPPK GNHINYQKVN SPSFCGLSYI GRVEPANPVY LSFQCGNGRG IAVHETMHAL 430 440 450 460 470 480 GVNHQHLRMD RDKHIKVDWS NINPQQYDAF VVADSKLYTT YGVKYAYDSI MHYNAYTGAV 490 500 510 520 530 540 NIAKPTMIPL VNQQANIGLL GQRAKMSNAD VEILNKMYCK SAGCDDKNVY CGAWALQDLC 550 560 NNPNHNVWMR SNCRKSCNFC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"The astacin protein family in Caenorhabditis elegans." Moehrlen F., Hutter H., Zwilling R. Eur. J. Biochem. 270:4909-4920(2003) [PubMed: 14653817] [Abstract] Cited for: NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z68006 Genomic DNA. Translation: CAA91996.1.
PIR	T23540.
RefSeq	NP_509832.1.
UniGene	Cel.696
3D structure databases
HSSP	HSSP built from PDB template 1IAE based on UniProtKB P07584.
ModBase	Search...
Protein family/group databases
MEROPS	M12.323.
Genome annotation databases
Ensembl	K09C8.3. Caenorhabditis elegans. [Contig view]
GeneID	181287.
KEGG	cel:K09C8.3.
NMPDR	fig|6239.3.peg.24545.
Organism-specific databases
WormBase	WBGene00003529. nas-10.
WormPep	K09C8.3. CE03479. [WorfDB]
Phylogenomic databases
OMA	Q21388. GRVEPAN.
Enzyme and pathway databases
BRENDA	3.4.24.21. 672.
Gene expression databases
ArrayExpress	Q21388.
Family and domain databases
InterPro	IPR006025. Pept_M_Zn_BS. IPR006026. Peptidase_M. IPR001506. Peptidase_M12A. IPR017368. Peptidase_M12A_astacin-9/10/11. IPR003582. ShK_toxin. [Graphical view]
Pfam	PF01400. Astacin. 1 hit. PF01549. ShK. 1 hit. [Graphical view]
PIRSF	PIRSF038055. Nas9/Nas10/Nas11. 1 hit.
PRINTS	PR00480. ASTACIN.
SMART	SM00254. ShKT. 1 hit. SM00235. ZnMc. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	913288.

Entry information

Entry name

NAS10_CAEEL

Accession

Primary (citable) accession number: Q21388

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project