Q21252 (NAS3_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc metalloproteinase nas-3
EC=3.4.24.21

Alternative name(s):
Nematode astacin 3

Gene names

Name:	nas-3
ORF Names:	K06A4.1

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probable metalloprotease By similarity.
Catalytic activity	Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the peptidase M12A family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 292	276	Zinc metalloproteinase nas-3		PRO_0000028908
Sites
	Active site	170	1	By similarity
	Metal binding	169	1	Zinc; catalytic By similarity
	Metal binding	173	1	Zinc; catalytic By similarity
	Metal binding	179	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q21252 [UniParc]. Last modified July 5, 2004. Version 2. Checksum: 0CDC6A600AB5EC48	FASTA	292	34,400
	10 20 30 40 50 60 MYRFIIFFSL LALTASKVSE PEKDDEIAVK IPTKRSVSEP PKDDDIAVKI PMRKKRGIAI 70 80 90 100 110 120 HPWQWESHLW PNAEVPYDIA SHYTATERGI ILSAMEAFRD VTCVRFRPRR STDKHYLQIN 130 140 150 160 170 180 KHYQLERCFS YIGRQSSRWL FGTRDGKVET RMKLDPSCLL YNGRGTVMHE LMHILGFYHE 190 200 210 220 230 240 HQRDDRDRRI GGSASHYNFK IYQRAKSYYM GGYDANSIMH YNFGSVPWQK RDYFSPSDIR 250 260 270 280 290 NINTLYKCNN RVVSKFPSTI PSTSTTTTKA PQFELFEKKQ IESNSLFRRR RS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"The astacin protein family in Caenorhabditis elegans." Moehrlen F., Hutter H., Zwilling R. Eur. J. Biochem. 270:4909-4920(2003) [PubMed: 14653817] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 128-207, NOMENCLATURE. Strain: Bristol N2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z70755 Genomic DNA. Translation: CAA94781.2. AJ561200 mRNA. Translation: CAD99180.1.
PIR	T23354.
RefSeq	NP_505445.2. NM_073044.3.
UniGene	Cel.3128.
3D structure databases
ProteinModelPortal	Q21252.
SMR	Q21252. Positions 35-249.
ModBase	Search...
Protein family/group databases
MEROPS	M12.A16.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	K06A4.1; K06A4.1; K06A4.1.
GeneID	187045.
KEGG	cel:K06A4.1.
NMPDR	fig|6239.3.peg.19110.
UCSC	K06A4.1. c. elegans.
Organism-specific databases
CTD	187045.
WormBase	K06A4.1; CE35894; WBGene00003522; nas-3.
Phylogenomic databases
eggNOG	KOG3714.
GeneTree	EMGT00050000002037.
HOGENOM	HBG386290.
InParanoid	Q21252.
OMA	VKIPTKR.
PhylomeDB	Q21252.
Family and domain databases
InterPro	IPR024079. MetalloPept_cat_dom. IPR001506. Peptidase_M12A. IPR006026. Peptidase_Metallo. [Graphical view]
Gene3D	G3DSA:3.40.390.10. G3DSA:3.40.390.10. 1 hit.
KO	K08076.
Pfam	PF01400. Astacin. 1 hit. [Graphical view]
PRINTS	PR00480. ASTACIN.
SMART	SM00235. ZnMc. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	933896.

Entry information

Entry name

NAS3_CAEEL

Accession

Primary (citable) accession number: Q21252
Secondary accession number(s): Q7Z0P2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	July 5, 2004
Last modified:	December 14, 2011
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project