Q21227 (APR1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenomatous polyposis coli protein-related protein 1
Short name=APC-related protein 1

Gene names

Name:	apr-1
ORF Names:	K04G2.8

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	1188 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has a role in endoderm cell specification and pharyngeal development. Required for the migration of epithelial cells, organization of the anterior seam cells and ceh-13 expression during embryo morphogenesis. During larval development, apr-1 is required for expression of lin-39 in P3-8.p. Shown to negatively regulate Wnt signaling in vulval precursor cells. Has a role in cell division by establishing the polarity of the mother cell which forms the asymmetries of the daughter nuclei. Thought to regulate export of wrm-1 from the nucleus possibly as part of a complex involving pry-1. Ref.1 Ref.2 Ref.3 Ref.4 Ref.5 Ref.7
Subunit structure	Interacts (via N-terminus) with bar-1 and hmp-2; the interaction with hmp-2 is relatively weak. Interacts (via C-terminus) with pry-1 (via N-terminus). Probably associates with bar-1, gsk-3, pry-1 in a complex. Ref.4 Ref.6
Subcellular location	Cell junction › adherens junction. Cytoplasm. Nucleus. Note: Found in clusters near the ends of microtubules that extend into regions of actively migrating plasma membranes. Shuttles between the cytoplasm and nucleus. Ref.2 Ref.3 Ref.7
Tissue specificity	Duing the L1 stage, expressed in vulval precursor cells (P3-8.p), seam cells and excretory cells. Ref.2 Ref.3 Ref.7
Disruption phenotype	Worms exhibit lack of endoderm, excessive pharyngeal tissue and premature division of the E daughter blastomeres during embryogenesis. Two-thirds arrest during embryogenesis and the remaining third during the L1 stage. Ref.1
Sequence similarities	Belongs to the adenomatous polyposis coli (APC) family. Contains 1 ARM repeat.

Ontologies

Keywords
Biological process	Cell cycle Cell division Protein transport Transport Wnt signaling pathway
Cellular component	Cell junction Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW asymmetric cell division Inferred from mutant phenotype Ref.7. Source: UniProtKB cell cycle Inferred from electronic annotation. Source: UniProtKB-KW embryo development ending in birth or egg hatching Inferred from mutant phenotype Ref.3Ref.5Ref.1. Source: WormBase endodermal cell fate specification Inferred from mutant phenotype Ref.1. Source: WormBase engulfment of apoptotic cell Inferred from mutant phenotype PubMed 20126385. Source: WormBase negative regulation of Wnt receptor signaling pathway Inferred from mutant phenotype Ref.5. Source: UniProtKB negative regulation of vulval development Inferred from genetic interaction Ref.5. Source: WormBase positive regulation of Wnt receptor signaling pathway Inferred from genetic interaction Ref.3. Source: WormBase positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype Ref.3. Source: WormBase protein transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell migration Inferred from mutant phenotype Ref.3. Source: WormBase regulation of embryonic cell shape Inferred from mutant phenotype Ref.3. Source: WormBase reproduction Inferred from mutant phenotype Ref.3. Source: WormBase vulval development Inferred from mutant phenotype Ref.3. Source: WormBase
Cellular_component	adherens junction Inferred from direct assay Ref.3. Source: UniProtKB cell cortex Inferred from direct assay Ref.7. Source: UniProtKB nucleus Inferred from direct assay Ref.7. Source: WormBase
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform b Ref.2 (identifier: Q21227-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform a Ref.1 (identifier: Q21227-2)

The sequence of this isoform differs from the canonical sequence as follows:
     552-553: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1188	1188	Adenomatous polyposis coli protein-related protein 1		PRO_0000347250
Regions
	Repeat	314 – 358	45	ARM
	Region	1 – 486	486	Required for interaction with bar-1 and hmp-2 Ref.4
	Region	600 – 1188	589	Required for interaction with pry-1 Ref.6
	Compositional bias	491 – 589	99	Gln-rich
	Compositional bias	953 – 996	44	Asp-rich
Natural variations
	Alternative sequence	552 – 553	2	Missing in isoform a. Ref.1		VSP_052859

Sequences

	Sequence		Length	Mass (Da)
	Isoform b [UniParc]. Last modified September 2, 2008. Version 2. Checksum: F3CDA53B5D3527A0	FASTA	1,188	131,990
	10 20 30 40 50 60 MSSSSSDENE TTIHRTGSNT GGSGIYSQPR AGSSKRTSNV RHDVSDVDDE EEHYARFRED 70 80 90 100 110 120 TAIEVDDAIT VLLSSLHFEH KRDIVPTDED DNKLRELHEK IFALITSESD VNRKRRLKKA 130 140 150 160 170 180 LPASNCVREQ VYYLRRKPST PPASYYHRLN AALHTIVKES FGEEYRKVAT VLGLVEALAE 190 200 210 220 230 240 VLILEVHTFG INETNPGEHR NIRKLIANAL TNLTYGQIHS KRRLCSYDGF IRCVVRIVIE 250 260 270 280 290 300 SPNITQVYAG LIRNLSWNAD SGMSEALQPT VHALSIAAVH AHTHRFDVTA TLSALWNLAG 310 320 330 340 350 360 HSVENKRTIC DTPNCLKVLA SLLSPDARFT SLVDSATGIL KYVSQYLANT STHLELRSLL 370 380 390 400 410 420 ITRMLTLLKS ASFTCVTNTL GAIANLIVKD PHMQQMIRQD MAAVQQLNVL RNSNRDDIRT 430 440 450 460 470 480 AVKSVLNTLN QPCSHRYGDM SHSVGGGATG MQMLSEPQLQ MQTSHHAYHG TASPRLLSLR 490 500 510 520 530 540 ATRASPGKYI QPQAQQQLIQ TPQVDQRSSS LPRHFAVQRN GFVMAQSYNQ QMDQHQQQQM 550 560 570 580 590 600 IYQLQQQQQI MFQTEDQAQM EHHQQIMYLQ QQQQQFHQIQ QQQQMQKAQE ADPVPPTDDD 610 620 630 640 650 660 LDIPTSTVMG TRSNSERSLG SMNPGSVMTN WNSSLDTAAN SSRALSPVSY NDIPASPTMC 670 680 690 700 710 720 AQVFNLPKST ESEHHQLTSQ QQNTTHYSSG SANTMTRSDG ATTVPMDNII TPTYAILNPI 730 740 750 760 770 780 LVHEQTPNGT VPRKTSEELD SPDDVLPGPS LEEEEGDYAI IGGAAQKTDD ELLTRSIQSE 790 800 810 820 830 840 MPTSSSTPKM KVSPRLNGFF SPTQKTTSSP AWSHPDTSPI PKSSSHRTQP NRRQDASDAD 850 860 870 880 890 900 RLLMESIMSE MPKSRIISPR LAGTQQYLEP EPERRSHSKN EEADRRDAFT ASHEPSDHNG 910 920 930 940 950 960 IDVARGSDWS PQQQLHRMES LESQASSEDS FGLTAEEPNS STSGAAANTM RFDDEIDASL 970 980 990 1000 1010 1020 PMDCVDDDDY DYTYDHFEDY EDEEDPDATQ FDDGVDAQLT IDCSMISSGS GSSQRNETTT 1030 1040 1050 1060 1070 1080 TSRDSKALAT STPKGSASSL PGVRQATRVS TNGKSRLPVP KTNGSLVDKN PKPIIASRRP 1090 1100 1110 1120 1130 1140 RLPPKPTLLK DKHYPEEDSI ENQTRDDTIY VNAPVVEAEQ ERIYMNALKQ QKNIEQSPSI 1150 1160 1170 1180 GNGSPIAKSA IVTPYNYQKP PFTGRNNGEM SNEKSVTPNP KQMLVTIV
	Isoform a [UniParc]. Checksum: CBE672D78E2B245D	FASTA	1,186	131,714
	10 20 30 40 50 60 MSSSSSDENE TTIHRTGSNT GGSGIYSQPR AGSSKRTSNV RHDVSDVDDE EEHYARFRED 70 80 90 100 110 120 TAIEVDDAIT VLLSSLHFEH KRDIVPTDED DNKLRELHEK IFALITSESD VNRKRRLKKA 130 140 150 160 170 180 LPASNCVREQ VYYLRRKPST PPASYYHRLN AALHTIVKES FGEEYRKVAT VLGLVEALAE 190 200 210 220 230 240 VLILEVHTFG INETNPGEHR NIRKLIANAL TNLTYGQIHS KRRLCSYDGF IRCVVRIVIE 250 260 270 280 290 300 SPNITQVYAG LIRNLSWNAD SGMSEALQPT VHALSIAAVH AHTHRFDVTA TLSALWNLAG 310 320 330 340 350 360 HSVENKRTIC DTPNCLKVLA SLLSPDARFT SLVDSATGIL KYVSQYLANT STHLELRSLL 370 380 390 400 410 420 ITRMLTLLKS ASFTCVTNTL GAIANLIVKD PHMQQMIRQD MAAVQQLNVL RNSNRDDIRT 430 440 450 460 470 480 AVKSVLNTLN QPCSHRYGDM SHSVGGGATG MQMLSEPQLQ MQTSHHAYHG TASPRLLSLR 490 500 510 520 530 540 ATRASPGKYI QPQAQQQLIQ TPQVDQRSSS LPRHFAVQRN GFVMAQSYNQ QMDQHQQQQM 550 560 570 580 590 600 IYQLQQQQQI MTEDQAQMEH HQQIMYLQQQ QQQFHQIQQQ QQMQKAQEAD PVPPTDDDLD 610 620 630 640 650 660 IPTSTVMGTR SNSERSLGSM NPGSVMTNWN SSLDTAANSS RALSPVSYND IPASPTMCAQ 670 680 690 700 710 720 VFNLPKSTES EHHQLTSQQQ NTTHYSSGSA NTMTRSDGAT TVPMDNIITP TYAILNPILV 730 740 750 760 770 780 HEQTPNGTVP RKTSEELDSP DDVLPGPSLE EEEGDYAIIG GAAQKTDDEL LTRSIQSEMP 790 800 810 820 830 840 TSSSTPKMKV SPRLNGFFSP TQKTTSSPAW SHPDTSPIPK SSSHRTQPNR RQDASDADRL 850 860 870 880 890 900 LMESIMSEMP KSRIISPRLA GTQQYLEPEP ERRSHSKNEE ADRRDAFTAS HEPSDHNGID 910 920 930 940 950 960 VARGSDWSPQ QQLHRMESLE SQASSEDSFG LTAEEPNSST SGAAANTMRF DDEIDASLPM 970 980 990 1000 1010 1020 DCVDDDDYDY TYDHFEDYED EEDPDATQFD DGVDAQLTID CSMISSGSGS SQRNETTTTS 1030 1040 1050 1060 1070 1080 RDSKALATST PKGSASSLPG VRQATRVSTN GKSRLPVPKT NGSLVDKNPK PIIASRRPRL 1090 1100 1110 1120 1130 1140 PPKPTLLKDK HYPEEDSIEN QTRDDTIYVN APVVEAEQER IYMNALKQQK NIEQSPSIGN 1150 1160 1170 1180 GSPIAKSAIV TPYNYQKPPF TGRNNGEMSN EKSVTPNPKQ MLVTIV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Wnt signaling and an APC-related gene specify endoderm in early C. elegans embryos." Rocheleau C.E., Downs W.D., Lin R., Wittmann C., Bei Y., Cha Y.-H., Ali M., Priess J.R., Mello C.C. Cell 90:707-716(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, DISRUPTION PHENOTYPE. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
[3]	"The Caenorhabditis elegans APC-related gene apr-1 is required for epithelial cell migration and Hox gene expression." Hoier E.F., Mohler W.A., Kim S.K., Hajnal A. Genes Dev. 14:874-886(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[4]	"The divergent Caenorhabditis elegans beta-catenin proteins BAR-1, WRM-1 and HMP-2 make distinct protein interactions but retain functional redundancy in vivo." Natarajan L., Witwer N.E., Eisenmann D.M. Genetics 159:159-172(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH BAR-1 AND HMP-2.
[5]	"Activation of Wnt signaling bypasses the requirement for RTK/Ras signaling during C. elegans vulval induction." Gleason J.E., Korswagen H.C., Eisenmann D.M. Genes Dev. 16:1281-1290(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"The axin-like protein PRY-1 is a negative regulator of a canonical Wnt pathway in C. elegans." Korswagen H.C., Coudreuse D.Y.M., Betist M.C., van de Water S., Zivkovic D., Clevers H.C. Genes Dev. 16:1291-1302(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PRY-1.
[7]	"Cortical beta-catenin and APC regulate asymmetric nuclear beta-catenin localization during asymmetric cell division in C. elegans." Mizumoto K., Sawa H. Dev. Cell 12:287-299(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF013950 mRNA. Translation: AAC47747.1. Z75712 Genomic DNA. Translation: CAB00045.1. Z75712 Genomic DNA. Translation: CAB00048.1.
PIR	T23327. T23330.
RefSeq	NP_001021547.1. NM_001026376.4. NP_492217.2. NM_059816.4.
UniGene	Cel.16875.
3D structure databases
ProteinModelPortal	Q21227.
SMR	Q21227. Positions 69-432.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-41257N.
IntAct	Q21227. 5 interactions.
MINT	MINT-214629.
Proteomic databases
PaxDb	Q21227.
PRIDE	Q21227.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	K04G2.8b; K04G2.8b; K04G2.8.
GeneID	172591.
KEGG	cel:CELE_K04G2.8.
UCSC	K04G2.8b. c. elegans.
Organism-specific databases
CTD	172591.
WormBase	K04G2.8a; CE06102; WBGene00000156; apr-1. K04G2.8b; CE18016; WBGene00000156; apr-1.
Phylogenomic databases
eggNOG	NOG259696.
GeneTree	ENSGT00530000063749.
HOGENOM	HOG000034014.
InParanoid	Q21227.
OMA	DGFIRCV.
Family and domain databases
Gene3D	1.25.10.10. 1 hit.
InterPro	IPR026818. Apc_fam. IPR026833. APR-1. IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR000225. Armadillo. [Graphical view]
PANTHER	PTHR12607. PTHR12607. 1 hit. PTHR12607:SF4. PTHR12607:SF4. 1 hit.
SMART	SM00185. ARM. 3 hits. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS50176. ARM_REPEAT. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	876155.

Entry information

Entry name

APR1_CAEEL

Accession

Primary (citable) accession number: Q21227
Secondary accession number(s): O62302

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	September 2, 2008
Last modified:	May 1, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project