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Q21227

- APR1_CAEEL

UniProt

Q21227 - APR1_CAEEL

Protein

Adenomatous polyposis coli protein-related protein 1

Gene

apr-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 2 (02 Sep 2008)
      Previous versions | rss
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    Functioni

    Has a role in endoderm cell specification and pharyngeal development. Required for the migration of epithelial cells, organization of the anterior seam cells and ceh-13 expression during embryo morphogenesis. During larval development, apr-1 is required for expression of lin-39 in P3-8.p. Shown to negatively regulate Wnt signaling in vulval precursor cells. Has a role in cell division by establishing the polarity of the mother cell which forms the asymmetries of the daughter nuclei. Thought to regulate export of wrm-1 from the nucleus possibly as part of a complex involving pry-1.4 Publications

    GO - Molecular functioni

    1. beta-catenin binding Source: WormBase
    2. protein binding Source: UniProtKB
    3. protein N-terminus binding Source: UniProtKB

    GO - Biological processi

    1. asymmetric cell division Source: UniProtKB
    2. cell cycle Source: UniProtKB-KW
    3. cell fate specification Source: WormBase
    4. embryo development ending in birth or egg hatching Source: WormBase
    5. embryonic morphogenesis Source: UniProtKB
    6. endodermal cell fate specification Source: WormBase
    7. endoderm development Source: UniProtKB
    8. engulfment of apoptotic cell Source: WormBase
    9. negative regulation of vulval development Source: WormBase
    10. negative regulation of Wnt signaling pathway Source: UniProtKB
    11. nematode larval development Source: WormBase
    12. positive regulation of transcription from RNA polymerase II promoter Source: WormBase
    13. positive regulation of Wnt signaling pathway Source: WormBase
    14. protein transport Source: UniProtKB-KW
    15. regulation of cell migration Source: WormBase
    16. regulation of embryonic cell shape Source: WormBase
    17. reproduction Source: WormBase
    18. vulval development Source: WormBase
    19. Wnt signaling pathway Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Cell cycle, Cell division, Protein transport, Transport, Wnt signaling pathway

    Enzyme and pathway databases

    SignaLinkiQ21227.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenomatous polyposis coli protein-related protein 1
    Short name:
    APC-related protein 1
    Gene namesi
    Name:apr-1Imported
    ORF Names:K04G2.8
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome I

    Organism-specific databases

    WormBaseiK04G2.8a; CE06102; WBGene00000156; apr-1.
    K04G2.8b; CE18016; WBGene00000156; apr-1.

    Subcellular locationi

    Cell junctionadherens junction 2 Publications. Cytoplasm 2 Publications. Nucleus 2 Publications
    Note: Found in clusters near the ends of microtubules that extend into regions of actively migrating plasma membranes. Shuttles between the cytoplasm and nucleus.2 Publications

    GO - Cellular componenti

    1. adherens junction Source: UniProtKB
    2. cell cortex Source: UniProtKB
    3. cytoplasm Source: UniProtKB
    4. nucleus Source: WormBase

    Keywords - Cellular componenti

    Cell junction, Cytoplasm, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Worms exhibit lack of endoderm, excessive pharyngeal tissue and premature division of the E daughter blastomeres during embryogenesis. Two-thirds arrest during embryogenesis and the remaining third during the L1 stage.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11881188Adenomatous polyposis coli protein-related protein 1PRO_0000347250Add
    BLAST

    Proteomic databases

    PaxDbiQ21227.
    PRIDEiQ21227.

    Expressioni

    Tissue specificityi

    Duing the L1 stage, expressed in vulval precursor cells (P3-8.p), seam cells and excretory cells.2 Publications

    Interactioni

    Subunit structurei

    Interacts (via N-terminus) with bar-1 and hmp-2; the interaction with hmp-2 is relatively weak. Interacts (via C-terminus) with pry-1 (via N-terminus). Probably associates with bar-1, gsk-3, pry-1 in a complex.2 Publications

    Protein-protein interaction databases

    BioGridi38025. 7 interactions.
    DIPiDIP-41257N.
    IntActiQ21227. 5 interactions.
    MINTiMINT-214629.

    Structurei

    3D structure databases

    ProteinModelPortaliQ21227.
    SMRiQ21227. Positions 69-432.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati314 – 35845ARMSequence AnalysisAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 486486Required for interaction with bar-1 and hmp-21 PublicationAdd
    BLAST
    Regioni600 – 1188589Required for interaction with pry-11 PublicationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi491 – 58999Gln-richSequence AnalysisAdd
    BLAST
    Compositional biasi953 – 99644Asp-richSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the adenomatous polyposis coli (APC) family.Sequence Analysis
    Contains 1 ARM repeat.Sequence Analysis

    Phylogenomic databases

    eggNOGiNOG259696.
    GeneTreeiENSGT00530000063749.
    HOGENOMiHOG000034014.
    InParanoidiQ21227.
    OMAiDGFIRCV.
    OrthoDBiEOG7BCN9S.

    Family and domain databases

    Gene3Di1.25.10.10. 1 hit.
    InterProiIPR026818. Apc_fam.
    IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR000225. Armadillo.
    [Graphical view]
    PANTHERiPTHR12607. PTHR12607. 1 hit.
    SMARTiSM00185. ARM. 3 hits.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform b1 Publication (identifier: Q21227-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSSSSSDENE TTIHRTGSNT GGSGIYSQPR AGSSKRTSNV RHDVSDVDDE     50
    EEHYARFRED TAIEVDDAIT VLLSSLHFEH KRDIVPTDED DNKLRELHEK 100
    IFALITSESD VNRKRRLKKA LPASNCVREQ VYYLRRKPST PPASYYHRLN 150
    AALHTIVKES FGEEYRKVAT VLGLVEALAE VLILEVHTFG INETNPGEHR 200
    NIRKLIANAL TNLTYGQIHS KRRLCSYDGF IRCVVRIVIE SPNITQVYAG 250
    LIRNLSWNAD SGMSEALQPT VHALSIAAVH AHTHRFDVTA TLSALWNLAG 300
    HSVENKRTIC DTPNCLKVLA SLLSPDARFT SLVDSATGIL KYVSQYLANT 350
    STHLELRSLL ITRMLTLLKS ASFTCVTNTL GAIANLIVKD PHMQQMIRQD 400
    MAAVQQLNVL RNSNRDDIRT AVKSVLNTLN QPCSHRYGDM SHSVGGGATG 450
    MQMLSEPQLQ MQTSHHAYHG TASPRLLSLR ATRASPGKYI QPQAQQQLIQ 500
    TPQVDQRSSS LPRHFAVQRN GFVMAQSYNQ QMDQHQQQQM IYQLQQQQQI 550
    MFQTEDQAQM EHHQQIMYLQ QQQQQFHQIQ QQQQMQKAQE ADPVPPTDDD 600
    LDIPTSTVMG TRSNSERSLG SMNPGSVMTN WNSSLDTAAN SSRALSPVSY 650
    NDIPASPTMC AQVFNLPKST ESEHHQLTSQ QQNTTHYSSG SANTMTRSDG 700
    ATTVPMDNII TPTYAILNPI LVHEQTPNGT VPRKTSEELD SPDDVLPGPS 750
    LEEEEGDYAI IGGAAQKTDD ELLTRSIQSE MPTSSSTPKM KVSPRLNGFF 800
    SPTQKTTSSP AWSHPDTSPI PKSSSHRTQP NRRQDASDAD RLLMESIMSE 850
    MPKSRIISPR LAGTQQYLEP EPERRSHSKN EEADRRDAFT ASHEPSDHNG 900
    IDVARGSDWS PQQQLHRMES LESQASSEDS FGLTAEEPNS STSGAAANTM 950
    RFDDEIDASL PMDCVDDDDY DYTYDHFEDY EDEEDPDATQ FDDGVDAQLT 1000
    IDCSMISSGS GSSQRNETTT TSRDSKALAT STPKGSASSL PGVRQATRVS 1050
    TNGKSRLPVP KTNGSLVDKN PKPIIASRRP RLPPKPTLLK DKHYPEEDSI 1100
    ENQTRDDTIY VNAPVVEAEQ ERIYMNALKQ QKNIEQSPSI GNGSPIAKSA 1150
    IVTPYNYQKP PFTGRNNGEM SNEKSVTPNP KQMLVTIV 1188

    Note: No experimental confirmation available.Curated

    Length:1,188
    Mass (Da):131,990
    Last modified:September 2, 2008 - v2
    Checksum:iF3CDA53B5D3527A0
    GO
    Isoform a1 Publication (identifier: Q21227-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         552-553: Missing.

    Show »
    Length:1,186
    Mass (Da):131,714
    Checksum:iCBE672D78E2B245D
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei552 – 5532Missing in isoform a. 1 PublicationVSP_052859

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF013950 mRNA. Translation: AAC47747.1.
    Z75712 Genomic DNA. Translation: CAB00045.1.
    Z75712 Genomic DNA. Translation: CAB00048.1.
    PIRiT23327.
    T23330.
    RefSeqiNP_001021547.1. NM_001026376.4. [Q21227-1]
    NP_492217.2. NM_059816.4. [Q21227-2]
    UniGeneiCel.16875.

    Genome annotation databases

    EnsemblMetazoaiK04G2.8b; K04G2.8b; WBGene00000156. [Q21227-1]
    GeneIDi172591.
    KEGGicel:CELE_K04G2.8.
    UCSCiK04G2.8b. c. elegans. [Q21227-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF013950 mRNA. Translation: AAC47747.1 .
    Z75712 Genomic DNA. Translation: CAB00045.1 .
    Z75712 Genomic DNA. Translation: CAB00048.1 .
    PIRi T23327.
    T23330.
    RefSeqi NP_001021547.1. NM_001026376.4. [Q21227-1 ]
    NP_492217.2. NM_059816.4. [Q21227-2 ]
    UniGenei Cel.16875.

    3D structure databases

    ProteinModelPortali Q21227.
    SMRi Q21227. Positions 69-432.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 38025. 7 interactions.
    DIPi DIP-41257N.
    IntActi Q21227. 5 interactions.
    MINTi MINT-214629.

    Proteomic databases

    PaxDbi Q21227.
    PRIDEi Q21227.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai K04G2.8b ; K04G2.8b ; WBGene00000156 . [Q21227-1 ]
    GeneIDi 172591.
    KEGGi cel:CELE_K04G2.8.
    UCSCi K04G2.8b. c. elegans. [Q21227-1 ]

    Organism-specific databases

    CTDi 172591.
    WormBasei K04G2.8a ; CE06102 ; WBGene00000156 ; apr-1.
    K04G2.8b ; CE18016 ; WBGene00000156 ; apr-1.

    Phylogenomic databases

    eggNOGi NOG259696.
    GeneTreei ENSGT00530000063749.
    HOGENOMi HOG000034014.
    InParanoidi Q21227.
    OMAi DGFIRCV.
    OrthoDBi EOG7BCN9S.

    Enzyme and pathway databases

    SignaLinki Q21227.

    Miscellaneous databases

    NextBioi 876155.

    Family and domain databases

    Gene3Di 1.25.10.10. 1 hit.
    InterProi IPR026818. Apc_fam.
    IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR000225. Armadillo.
    [Graphical view ]
    PANTHERi PTHR12607. PTHR12607. 1 hit.
    SMARTi SM00185. ARM. 3 hits.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Wnt signaling and an APC-related gene specify endoderm in early C. elegans embryos."
      Rocheleau C.E., Downs W.D., Lin R., Wittmann C., Bei Y., Cha Y.-H., Ali M., Priess J.R., Mello C.C.
      Cell 90:707-716(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, DISRUPTION PHENOTYPE.
      Strain: Bristol N2Imported.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: Bristol N2.
    3. "The Caenorhabditis elegans APC-related gene apr-1 is required for epithelial cell migration and Hox gene expression."
      Hoier E.F., Mohler W.A., Kim S.K., Hajnal A.
      Genes Dev. 14:874-886(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    4. "The divergent Caenorhabditis elegans beta-catenin proteins BAR-1, WRM-1 and HMP-2 make distinct protein interactions but retain functional redundancy in vivo."
      Natarajan L., Witwer N.E., Eisenmann D.M.
      Genetics 159:159-172(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH BAR-1 AND HMP-2.
    5. "Activation of Wnt signaling bypasses the requirement for RTK/Ras signaling during C. elegans vulval induction."
      Gleason J.E., Korswagen H.C., Eisenmann D.M.
      Genes Dev. 16:1281-1290(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "The axin-like protein PRY-1 is a negative regulator of a canonical Wnt pathway in C. elegans."
      Korswagen H.C., Coudreuse D.Y.M., Betist M.C., van de Water S., Zivkovic D., Clevers H.C.
      Genes Dev. 16:1291-1302(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PRY-1.
    7. "Cortical beta-catenin and APC regulate asymmetric nuclear beta-catenin localization during asymmetric cell division in C. elegans."
      Mizumoto K., Sawa H.
      Dev. Cell 12:287-299(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiAPR1_CAEEL
    AccessioniPrimary (citable) accession number: Q21227
    Secondary accession number(s): O62302
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 2, 2008
    Last sequence update: September 2, 2008
    Last modified: October 1, 2014
    This is version 98 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3