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Q21227

- APR1_CAEEL

UniProt

Q21227 - APR1_CAEEL

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Protein

Adenomatous polyposis coli protein-related protein 1

Gene

apr-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Has a role in endoderm cell specification and pharyngeal development. Required for the migration of epithelial cells, organization of the anterior seam cells and ceh-13 expression during embryo morphogenesis. During larval development, apr-1 is required for expression of lin-39 in P3-8.p. Shown to negatively regulate Wnt signaling in vulval precursor cells. Has a role in cell division by establishing the polarity of the mother cell which forms the asymmetries of the daughter nuclei. Thought to regulate export of wrm-1 from the nucleus possibly as part of a complex involving pry-1.4 Publications

GO - Molecular functioni

  1. beta-catenin binding Source: WormBase
  2. protein N-terminus binding Source: UniProtKB

GO - Biological processi

  1. asymmetric cell division Source: UniProtKB
  2. cell cycle Source: UniProtKB-KW
  3. cell fate specification Source: WormBase
  4. embryo development ending in birth or egg hatching Source: WormBase
  5. embryonic morphogenesis Source: UniProtKB
  6. endodermal cell fate specification Source: WormBase
  7. endoderm development Source: UniProtKB
  8. engulfment of apoptotic cell Source: WormBase
  9. negative regulation of vulval development Source: WormBase
  10. negative regulation of Wnt signaling pathway Source: UniProtKB
  11. nematode larval development Source: WormBase
  12. positive regulation of transcription from RNA polymerase II promoter Source: WormBase
  13. positive regulation of Wnt signaling pathway Source: WormBase
  14. protein transport Source: UniProtKB-KW
  15. regulation of cell migration Source: WormBase
  16. regulation of embryonic cell shape Source: WormBase
  17. reproduction Source: WormBase
  18. vulval development Source: WormBase
  19. Wnt signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Cell cycle, Cell division, Protein transport, Transport, Wnt signaling pathway

Enzyme and pathway databases

SignaLinkiQ21227.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenomatous polyposis coli protein-related protein 1
Short name:
APC-related protein 1
Gene namesi
Name:apr-1Imported
ORF Names:K04G2.8
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome I

Organism-specific databases

WormBaseiK04G2.8a; CE06102; WBGene00000156; apr-1.
K04G2.8b; CE18016; WBGene00000156; apr-1.

Subcellular locationi

Cell junctionadherens junction 2 Publications. Cytoplasm 2 Publications. Nucleus 2 Publications
Note: Found in clusters near the ends of microtubules that extend into regions of actively migrating plasma membranes. Shuttles between the cytoplasm and nucleus.2 Publications

GO - Cellular componenti

  1. adherens junction Source: UniProtKB
  2. cell cortex Source: UniProtKB
  3. cytoplasm Source: UniProtKB
  4. nucleus Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Worms exhibit lack of endoderm, excessive pharyngeal tissue and premature division of the E daughter blastomeres during embryogenesis. Two-thirds arrest during embryogenesis and the remaining third during the L1 stage.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11881188Adenomatous polyposis coli protein-related protein 1PRO_0000347250Add
BLAST

Proteomic databases

PaxDbiQ21227.
PRIDEiQ21227.

Expressioni

Tissue specificityi

Duing the L1 stage, expressed in vulval precursor cells (P3-8.p), seam cells and excretory cells.2 Publications

Interactioni

Subunit structurei

Interacts (via N-terminus) with bar-1 and hmp-2; the interaction with hmp-2 is relatively weak. Interacts (via C-terminus) with pry-1 (via N-terminus). Probably associates with bar-1, gsk-3, pry-1 in a complex.2 Publications

Protein-protein interaction databases

BioGridi38025. 7 interactions.
DIPiDIP-41257N.
IntActiQ21227. 5 interactions.
MINTiMINT-214629.

Structurei

3D structure databases

ProteinModelPortaliQ21227.
SMRiQ21227. Positions 69-432.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati314 – 35845ARMSequence AnalysisAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 486486Required for interaction with bar-1 and hmp-21 PublicationAdd
BLAST
Regioni600 – 1188589Required for interaction with pry-11 PublicationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi491 – 58999Gln-richSequence AnalysisAdd
BLAST
Compositional biasi953 – 99644Asp-richSequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the adenomatous polyposis coli (APC) family.Sequence Analysis
Contains 1 ARM repeat.Sequence Analysis

Phylogenomic databases

eggNOGiNOG259696.
GeneTreeiENSGT00530000063749.
HOGENOMiHOG000034014.
InParanoidiQ21227.
OMAiDGFIRCV.
OrthoDBiEOG7BCN9S.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR026818. Apc_fam.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
[Graphical view]
PANTHERiPTHR12607. PTHR12607. 1 hit.
SMARTiSM00185. ARM. 3 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform b1 Publication (identifier: Q21227-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSSSSDENE TTIHRTGSNT GGSGIYSQPR AGSSKRTSNV RHDVSDVDDE
60 70 80 90 100
EEHYARFRED TAIEVDDAIT VLLSSLHFEH KRDIVPTDED DNKLRELHEK
110 120 130 140 150
IFALITSESD VNRKRRLKKA LPASNCVREQ VYYLRRKPST PPASYYHRLN
160 170 180 190 200
AALHTIVKES FGEEYRKVAT VLGLVEALAE VLILEVHTFG INETNPGEHR
210 220 230 240 250
NIRKLIANAL TNLTYGQIHS KRRLCSYDGF IRCVVRIVIE SPNITQVYAG
260 270 280 290 300
LIRNLSWNAD SGMSEALQPT VHALSIAAVH AHTHRFDVTA TLSALWNLAG
310 320 330 340 350
HSVENKRTIC DTPNCLKVLA SLLSPDARFT SLVDSATGIL KYVSQYLANT
360 370 380 390 400
STHLELRSLL ITRMLTLLKS ASFTCVTNTL GAIANLIVKD PHMQQMIRQD
410 420 430 440 450
MAAVQQLNVL RNSNRDDIRT AVKSVLNTLN QPCSHRYGDM SHSVGGGATG
460 470 480 490 500
MQMLSEPQLQ MQTSHHAYHG TASPRLLSLR ATRASPGKYI QPQAQQQLIQ
510 520 530 540 550
TPQVDQRSSS LPRHFAVQRN GFVMAQSYNQ QMDQHQQQQM IYQLQQQQQI
560 570 580 590 600
MFQTEDQAQM EHHQQIMYLQ QQQQQFHQIQ QQQQMQKAQE ADPVPPTDDD
610 620 630 640 650
LDIPTSTVMG TRSNSERSLG SMNPGSVMTN WNSSLDTAAN SSRALSPVSY
660 670 680 690 700
NDIPASPTMC AQVFNLPKST ESEHHQLTSQ QQNTTHYSSG SANTMTRSDG
710 720 730 740 750
ATTVPMDNII TPTYAILNPI LVHEQTPNGT VPRKTSEELD SPDDVLPGPS
760 770 780 790 800
LEEEEGDYAI IGGAAQKTDD ELLTRSIQSE MPTSSSTPKM KVSPRLNGFF
810 820 830 840 850
SPTQKTTSSP AWSHPDTSPI PKSSSHRTQP NRRQDASDAD RLLMESIMSE
860 870 880 890 900
MPKSRIISPR LAGTQQYLEP EPERRSHSKN EEADRRDAFT ASHEPSDHNG
910 920 930 940 950
IDVARGSDWS PQQQLHRMES LESQASSEDS FGLTAEEPNS STSGAAANTM
960 970 980 990 1000
RFDDEIDASL PMDCVDDDDY DYTYDHFEDY EDEEDPDATQ FDDGVDAQLT
1010 1020 1030 1040 1050
IDCSMISSGS GSSQRNETTT TSRDSKALAT STPKGSASSL PGVRQATRVS
1060 1070 1080 1090 1100
TNGKSRLPVP KTNGSLVDKN PKPIIASRRP RLPPKPTLLK DKHYPEEDSI
1110 1120 1130 1140 1150
ENQTRDDTIY VNAPVVEAEQ ERIYMNALKQ QKNIEQSPSI GNGSPIAKSA
1160 1170 1180
IVTPYNYQKP PFTGRNNGEM SNEKSVTPNP KQMLVTIV

Note: No experimental confirmation available.Curated

Length:1,188
Mass (Da):131,990
Last modified:September 2, 2008 - v2
Checksum:iF3CDA53B5D3527A0
GO
Isoform a1 Publication (identifier: Q21227-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     552-553: Missing.

Show »
Length:1,186
Mass (Da):131,714
Checksum:iCBE672D78E2B245D
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei552 – 5532Missing in isoform a. 1 PublicationVSP_052859

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF013950 mRNA. Translation: AAC47747.1.
Z75712 Genomic DNA. Translation: CAB00045.1.
Z75712 Genomic DNA. Translation: CAB00048.1.
PIRiT23327.
T23330.
RefSeqiNP_001021547.1. NM_001026376.4. [Q21227-1]
NP_492217.2. NM_059816.4. [Q21227-2]
UniGeneiCel.16875.

Genome annotation databases

EnsemblMetazoaiK04G2.8b; K04G2.8b; WBGene00000156. [Q21227-1]
GeneIDi172591.
KEGGicel:CELE_K04G2.8.
UCSCiK04G2.8b. c. elegans. [Q21227-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF013950 mRNA. Translation: AAC47747.1 .
Z75712 Genomic DNA. Translation: CAB00045.1 .
Z75712 Genomic DNA. Translation: CAB00048.1 .
PIRi T23327.
T23330.
RefSeqi NP_001021547.1. NM_001026376.4. [Q21227-1 ]
NP_492217.2. NM_059816.4. [Q21227-2 ]
UniGenei Cel.16875.

3D structure databases

ProteinModelPortali Q21227.
SMRi Q21227. Positions 69-432.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 38025. 7 interactions.
DIPi DIP-41257N.
IntActi Q21227. 5 interactions.
MINTi MINT-214629.

Proteomic databases

PaxDbi Q21227.
PRIDEi Q21227.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai K04G2.8b ; K04G2.8b ; WBGene00000156 . [Q21227-1 ]
GeneIDi 172591.
KEGGi cel:CELE_K04G2.8.
UCSCi K04G2.8b. c. elegans. [Q21227-1 ]

Organism-specific databases

CTDi 172591.
WormBasei K04G2.8a ; CE06102 ; WBGene00000156 ; apr-1.
K04G2.8b ; CE18016 ; WBGene00000156 ; apr-1.

Phylogenomic databases

eggNOGi NOG259696.
GeneTreei ENSGT00530000063749.
HOGENOMi HOG000034014.
InParanoidi Q21227.
OMAi DGFIRCV.
OrthoDBi EOG7BCN9S.

Enzyme and pathway databases

SignaLinki Q21227.

Miscellaneous databases

NextBioi 876155.

Family and domain databases

Gene3Di 1.25.10.10. 1 hit.
InterProi IPR026818. Apc_fam.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
[Graphical view ]
PANTHERi PTHR12607. PTHR12607. 1 hit.
SMARTi SM00185. ARM. 3 hits.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Wnt signaling and an APC-related gene specify endoderm in early C. elegans embryos."
    Rocheleau C.E., Downs W.D., Lin R., Wittmann C., Bei Y., Cha Y.-H., Ali M., Priess J.R., Mello C.C.
    Cell 90:707-716(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, DISRUPTION PHENOTYPE.
    Strain: Bristol N2Imported.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  3. "The Caenorhabditis elegans APC-related gene apr-1 is required for epithelial cell migration and Hox gene expression."
    Hoier E.F., Mohler W.A., Kim S.K., Hajnal A.
    Genes Dev. 14:874-886(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  4. "The divergent Caenorhabditis elegans beta-catenin proteins BAR-1, WRM-1 and HMP-2 make distinct protein interactions but retain functional redundancy in vivo."
    Natarajan L., Witwer N.E., Eisenmann D.M.
    Genetics 159:159-172(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BAR-1 AND HMP-2.
  5. "Activation of Wnt signaling bypasses the requirement for RTK/Ras signaling during C. elegans vulval induction."
    Gleason J.E., Korswagen H.C., Eisenmann D.M.
    Genes Dev. 16:1281-1290(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "The axin-like protein PRY-1 is a negative regulator of a canonical Wnt pathway in C. elegans."
    Korswagen H.C., Coudreuse D.Y.M., Betist M.C., van de Water S., Zivkovic D., Clevers H.C.
    Genes Dev. 16:1291-1302(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PRY-1.
  7. "Cortical beta-catenin and APC regulate asymmetric nuclear beta-catenin localization during asymmetric cell division in C. elegans."
    Mizumoto K., Sawa H.
    Dev. Cell 12:287-299(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiAPR1_CAEEL
AccessioniPrimary (citable) accession number: Q21227
Secondary accession number(s): O62302
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: September 2, 2008
Last modified: October 29, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3