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Q21227 (APR1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Adenomatous polyposis coli protein-related protein 1

Short name=APC-related protein 1
Gene names
Name:apr-1
ORF Names:K04G2.8
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length1188 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has a role in endoderm cell specification and pharyngeal development. Required for the migration of epithelial cells, organization of the anterior seam cells and ceh-13 expression during embryo morphogenesis. During larval development, apr-1 is required for expression of lin-39 in P3-8.p. Shown to negatively regulate Wnt signaling in vulval precursor cells. Has a role in cell division by establishing the polarity of the mother cell which forms the asymmetries of the daughter nuclei. Thought to regulate export of wrm-1 from the nucleus possibly as part of a complex involving pry-1. Ref.1 Ref.2 Ref.3 Ref.4 Ref.5 Ref.7

Subunit structure

Interacts (via N-terminus) with bar-1 and hmp-2; the interaction with hmp-2 is relatively weak. Interacts (via C-terminus) with pry-1 (via N-terminus). Probably associates with bar-1, gsk-3, pry-1 in a complex. Ref.4 Ref.6

Subcellular location

Cell junctionadherens junction. Cytoplasm. Nucleus. Note: Found in clusters near the ends of microtubules that extend into regions of actively migrating plasma membranes. Shuttles between the cytoplasm and nucleus. Ref.2 Ref.3 Ref.7

Tissue specificity

Duing the L1 stage, expressed in vulval precursor cells (P3-8.p), seam cells and excretory cells. Ref.2 Ref.3 Ref.7

Disruption phenotype

Worms exhibit lack of endoderm, excessive pharyngeal tissue and premature division of the E daughter blastomeres during embryogenesis. Two-thirds arrest during embryogenesis and the remaining third during the L1 stage. Ref.1

Sequence similarities

Belongs to the adenomatous polyposis coli (APC) family.

Contains 1 ARM repeat.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Protein transport
Transport
Wnt signaling pathway
   Cellular componentCell junction
Cytoplasm
Nucleus
   Coding sequence diversityAlternative splicing
   Molecular functionDevelopmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processWnt signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

asymmetric cell division

Inferred from mutant phenotype Ref.7. Source: UniProtKB

cell cycle

Inferred from electronic annotation. Source: UniProtKB-KW

cell fate specification

Inferred from mutant phenotype Ref.3. Source: WormBase

embryo development ending in birth or egg hatching

Inferred from mutant phenotype Ref.3Ref.5Ref.1. Source: WormBase

embryonic morphogenesis

Inferred from mutant phenotype Ref.3. Source: UniProtKB

endoderm development

Inferred from mutant phenotype Ref.1. Source: UniProtKB

endodermal cell fate specification

Inferred from mutant phenotype Ref.1. Source: WormBase

engulfment of apoptotic cell

Inferred from mutant phenotype PubMed 20126385. Source: WormBase

negative regulation of Wnt signaling pathway

Inferred from mutant phenotype Ref.5. Source: UniProtKB

negative regulation of vulval development

Inferred from genetic interaction Ref.5. Source: WormBase

nematode larval development

Inferred from mutant phenotype Ref.3. Source: WormBase

positive regulation of Wnt signaling pathway

Inferred from genetic interaction Ref.3. Source: WormBase

positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype Ref.3. Source: WormBase

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of cell migration

Inferred from mutant phenotype Ref.3. Source: WormBase

regulation of embryonic cell shape

Inferred from mutant phenotype Ref.3. Source: WormBase

reproduction

Inferred from mutant phenotype Ref.3. Source: WormBase

vulval development

Inferred from mutant phenotype Ref.3. Source: WormBase

   Cellular_componentadherens junction

Inferred from direct assay Ref.3. Source: UniProtKB

cell cortex

Inferred from direct assay Ref.7. Source: UniProtKB

cytoplasm

Inferred from direct assay Ref.3. Source: UniProtKB

nucleus

Inferred from direct assay Ref.7. Source: WormBase

   Molecular_functionbeta-catenin binding

Inferred from physical interaction Ref.4. Source: WormBase

protein N-terminus binding

Inferred from physical interaction Ref.6. Source: UniProtKB

protein binding

Inferred from physical interaction Ref.4. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform b Ref.2 (identifier: Q21227-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform a Ref.1 (identifier: Q21227-2)

The sequence of this isoform differs from the canonical sequence as follows:
     552-553: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11881188Adenomatous polyposis coli protein-related protein 1
PRO_0000347250

Regions

Repeat314 – 35845ARM
Region1 – 486486Required for interaction with bar-1 and hmp-2 Ref.4
Region600 – 1188589Required for interaction with pry-1 Ref.6
Compositional bias491 – 58999Gln-rich
Compositional bias953 – 99644Asp-rich

Natural variations

Alternative sequence552 – 5532Missing in isoform a. Ref.1
VSP_052859

Sequences

Sequence LengthMass (Da)Tools
Isoform b [UniParc].

Last modified September 2, 2008. Version 2.
Checksum: F3CDA53B5D3527A0

FASTA1,188131,990
        10         20         30         40         50         60 
MSSSSSDENE TTIHRTGSNT GGSGIYSQPR AGSSKRTSNV RHDVSDVDDE EEHYARFRED 

        70         80         90        100        110        120 
TAIEVDDAIT VLLSSLHFEH KRDIVPTDED DNKLRELHEK IFALITSESD VNRKRRLKKA 

       130        140        150        160        170        180 
LPASNCVREQ VYYLRRKPST PPASYYHRLN AALHTIVKES FGEEYRKVAT VLGLVEALAE 

       190        200        210        220        230        240 
VLILEVHTFG INETNPGEHR NIRKLIANAL TNLTYGQIHS KRRLCSYDGF IRCVVRIVIE 

       250        260        270        280        290        300 
SPNITQVYAG LIRNLSWNAD SGMSEALQPT VHALSIAAVH AHTHRFDVTA TLSALWNLAG 

       310        320        330        340        350        360 
HSVENKRTIC DTPNCLKVLA SLLSPDARFT SLVDSATGIL KYVSQYLANT STHLELRSLL 

       370        380        390        400        410        420 
ITRMLTLLKS ASFTCVTNTL GAIANLIVKD PHMQQMIRQD MAAVQQLNVL RNSNRDDIRT 

       430        440        450        460        470        480 
AVKSVLNTLN QPCSHRYGDM SHSVGGGATG MQMLSEPQLQ MQTSHHAYHG TASPRLLSLR 

       490        500        510        520        530        540 
ATRASPGKYI QPQAQQQLIQ TPQVDQRSSS LPRHFAVQRN GFVMAQSYNQ QMDQHQQQQM 

       550        560        570        580        590        600 
IYQLQQQQQI MFQTEDQAQM EHHQQIMYLQ QQQQQFHQIQ QQQQMQKAQE ADPVPPTDDD 

       610        620        630        640        650        660 
LDIPTSTVMG TRSNSERSLG SMNPGSVMTN WNSSLDTAAN SSRALSPVSY NDIPASPTMC 

       670        680        690        700        710        720 
AQVFNLPKST ESEHHQLTSQ QQNTTHYSSG SANTMTRSDG ATTVPMDNII TPTYAILNPI 

       730        740        750        760        770        780 
LVHEQTPNGT VPRKTSEELD SPDDVLPGPS LEEEEGDYAI IGGAAQKTDD ELLTRSIQSE 

       790        800        810        820        830        840 
MPTSSSTPKM KVSPRLNGFF SPTQKTTSSP AWSHPDTSPI PKSSSHRTQP NRRQDASDAD 

       850        860        870        880        890        900 
RLLMESIMSE MPKSRIISPR LAGTQQYLEP EPERRSHSKN EEADRRDAFT ASHEPSDHNG 

       910        920        930        940        950        960 
IDVARGSDWS PQQQLHRMES LESQASSEDS FGLTAEEPNS STSGAAANTM RFDDEIDASL 

       970        980        990       1000       1010       1020 
PMDCVDDDDY DYTYDHFEDY EDEEDPDATQ FDDGVDAQLT IDCSMISSGS GSSQRNETTT 

      1030       1040       1050       1060       1070       1080 
TSRDSKALAT STPKGSASSL PGVRQATRVS TNGKSRLPVP KTNGSLVDKN PKPIIASRRP 

      1090       1100       1110       1120       1130       1140 
RLPPKPTLLK DKHYPEEDSI ENQTRDDTIY VNAPVVEAEQ ERIYMNALKQ QKNIEQSPSI 

      1150       1160       1170       1180 
GNGSPIAKSA IVTPYNYQKP PFTGRNNGEM SNEKSVTPNP KQMLVTIV 

« Hide

Isoform a [UniParc].

Checksum: CBE672D78E2B245D
Show »

FASTA1,186131,714

References

« Hide 'large scale' references
[1]"Wnt signaling and an APC-related gene specify endoderm in early C. elegans embryos."
Rocheleau C.E., Downs W.D., Lin R., Wittmann C., Bei Y., Cha Y.-H., Ali M., Priess J.R., Mello C.C.
Cell 90:707-716(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, DISRUPTION PHENOTYPE.
Strain: Bristol N2.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
[3]"The Caenorhabditis elegans APC-related gene apr-1 is required for epithelial cell migration and Hox gene expression."
Hoier E.F., Mohler W.A., Kim S.K., Hajnal A.
Genes Dev. 14:874-886(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[4]"The divergent Caenorhabditis elegans beta-catenin proteins BAR-1, WRM-1 and HMP-2 make distinct protein interactions but retain functional redundancy in vivo."
Natarajan L., Witwer N.E., Eisenmann D.M.
Genetics 159:159-172(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH BAR-1 AND HMP-2.
[5]"Activation of Wnt signaling bypasses the requirement for RTK/Ras signaling during C. elegans vulval induction."
Gleason J.E., Korswagen H.C., Eisenmann D.M.
Genes Dev. 16:1281-1290(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"The axin-like protein PRY-1 is a negative regulator of a canonical Wnt pathway in C. elegans."
Korswagen H.C., Coudreuse D.Y.M., Betist M.C., van de Water S., Zivkovic D., Clevers H.C.
Genes Dev. 16:1291-1302(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PRY-1.
[7]"Cortical beta-catenin and APC regulate asymmetric nuclear beta-catenin localization during asymmetric cell division in C. elegans."
Mizumoto K., Sawa H.
Dev. Cell 12:287-299(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF013950 mRNA. Translation: AAC47747.1.
Z75712 Genomic DNA. Translation: CAB00045.1.
Z75712 Genomic DNA. Translation: CAB00048.1.
PIRT23327.
T23330.
RefSeqNP_001021547.1. NM_001026376.4. [Q21227-1]
NP_492217.2. NM_059816.4. [Q21227-2]
UniGeneCel.16875.

3D structure databases

ProteinModelPortalQ21227.
SMRQ21227. Positions 69-432.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid38025. 7 interactions.
DIPDIP-41257N.
IntActQ21227. 5 interactions.
MINTMINT-214629.

Proteomic databases

PaxDbQ21227.
PRIDEQ21227.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaK04G2.8b; K04G2.8b; WBGene00000156. [Q21227-1]
GeneID172591.
KEGGcel:CELE_K04G2.8.
UCSCK04G2.8b. c. elegans. [Q21227-1]

Organism-specific databases

CTD172591.
WormBaseK04G2.8a; CE06102; WBGene00000156; apr-1.
K04G2.8b; CE18016; WBGene00000156; apr-1.

Phylogenomic databases

eggNOGNOG259696.
GeneTreeENSGT00530000063749.
HOGENOMHOG000034014.
InParanoidQ21227.
OMADGFIRCV.
OrthoDBEOG7BCN9S.

Enzyme and pathway databases

SignaLinkQ21227.

Family and domain databases

Gene3D1.25.10.10. 1 hit.
InterProIPR026818. Apc_fam.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
[Graphical view]
PANTHERPTHR12607. PTHR12607. 1 hit.
SMARTSM00185. ARM. 3 hits.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
ProtoNetSearch...

Other

NextBio876155.

Entry information

Entry nameAPR1_CAEEL
AccessionPrimary (citable) accession number: Q21227
Secondary accession number(s): O62302
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: September 2, 2008
Last modified: June 11, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase