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Protein

Adenomatous polyposis coli protein-related protein 1

Gene

apr-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in endoderm cell specification and pharyngeal development (PubMed:9288750). Required for the migration of epithelial cells, organization of the anterior seam cells and ceh-13 expression during embryo morphogenesis. Prevents hyperactivation of the Wnt signaling pathway during endoderm development, probably by preventing hmp-2 nuclear translocation (PubMed:20805471). During larval development, apr-1 is required for expression of lin-39 in P3-8.p (PubMed:10766743). Shown to negatively regulate Wnt signaling in vulval precursor cells (PubMed:12023306). Has a role in cell division by establishing the polarity of the mother cell which forms the asymmetries of the daughter nuclei (PubMed:17276345). Thought to regulate export of wrm-1 from the nucleus possibly as part of a complex involving pry-1 (PubMed:12023307).6 Publications

GO - Molecular functioni

  • beta-catenin binding Source: WormBase
  • protein N-terminus binding Source: UniProtKB

GO - Biological processi

  • asymmetric cell division Source: UniProtKB
  • cell cycle Source: UniProtKB-KW
  • cell fate specification Source: WormBase
  • embryo development ending in birth or egg hatching Source: WormBase
  • embryonic digestive tract morphogenesis Source: UniProtKB
  • embryonic morphogenesis Source: UniProtKB
  • endodermal cell fate specification Source: WormBase
  • endoderm development Source: UniProtKB
  • engulfment of apoptotic cell Source: WormBase
  • negative regulation of catenin import into nucleus Source: UniProtKB
  • negative regulation of cell division Source: UniProtKB
  • negative regulation of vulval development Source: WormBase
  • negative regulation of Wnt signaling pathway Source: UniProtKB
  • negative regulation of Wnt signaling pathway involved in digestive tract morphogenesis Source: UniProtKB
  • nematode larval development Source: WormBase
  • positive regulation of transcription from RNA polymerase II promoter Source: WormBase
  • positive regulation of Wnt signaling pathway Source: WormBase
  • protein transport Source: UniProtKB-KW
  • regulation of cell migration Source: WormBase
  • regulation of embryonic cell shape Source: WormBase
  • reproduction Source: WormBase
  • vulval development Source: WormBase
  • Wnt signaling pathway involved in digestive tract morphogenesis Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein
Biological processCell cycle, Cell division, Protein transport, Transport, Wnt signaling pathway

Enzyme and pathway databases

ReactomeiR-CEL-195253. Degradation of beta-catenin by the destruction complex.
R-CEL-3769402. Deactivation of the beta-catenin transactivating complex.
SignaLinkiQ21227.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenomatous polyposis coli protein-related protein 1
Short name:
APC-related protein 1
Gene namesi
Name:apr-1Imported
ORF Names:K04G2.8
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiK04G2.8a; CE06102; WBGene00000156; apr-1.
K04G2.8b; CE18016; WBGene00000156; apr-1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Worms exhibit lack of endoderm, excessive pharyngeal tissue and premature division of the E daughter blastomeres during embryogenesis. Two-thirds arrest during embryogenesis and the remaining third during the L1 stage (PubMed:9288750). RNAi-mediated knockdown causes partial nuclear re-localization of hmp-2 in the embryonic epidermis and the production of supernumerary gut nuclei probably resulting from epithelial cell hyperproliferation (PubMed:20805471).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003472501 – 1188Adenomatous polyposis coli protein-related protein 1Add BLAST1188

Proteomic databases

PaxDbiQ21227.
PeptideAtlasiQ21227.

Expressioni

Tissue specificityi

During the L1 stage, expressed in vulval precursor cells (P3-8.p), seam cells and excretory cells.2 Publications

Gene expression databases

BgeeiWBGene00000156.
ExpressionAtlasiQ21227. baseline.

Interactioni

Subunit structurei

Interacts (via N-terminus) with bar-1 and hmp-2; the interaction with hmp-2 is relatively weak. Interacts (via C-terminus) with pry-1 (via N-terminus). Probably associates with bar-1, gsk-3, pry-1 in a complex.2 Publications

GO - Molecular functioni

  • beta-catenin binding Source: WormBase
  • protein N-terminus binding Source: UniProtKB

Protein-protein interaction databases

BioGridi38025. 7 interactors.
DIPiDIP-41257N.
IntActiQ21227. 19 interactors.
MINTiMINT-214629.
STRINGi6239.K04G2.8b.

Structurei

3D structure databases

ProteinModelPortaliQ21227.
SMRiQ21227.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati314 – 358ARMSequence analysisAdd BLAST45

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 486Required for interaction with bar-1 and hmp-21 PublicationAdd BLAST486
Regioni600 – 1188Required for interaction with pry-11 PublicationAdd BLAST589

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi491 – 589Gln-richSequence analysisAdd BLAST99
Compositional biasi953 – 996Asp-richSequence analysisAdd BLAST44

Sequence similaritiesi

Belongs to the adenomatous polyposis coli (APC) family.Sequence analysis

Phylogenomic databases

eggNOGiKOG2122. Eukaryota.
ENOG410XR2V. LUCA.
GeneTreeiENSGT00530000063749.
HOGENOMiHOG000034014.
InParanoidiQ21227.
OMAiGHSVENK.
OrthoDBiEOG091G00D4.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
InterProiView protein in InterPro
IPR026818. Apc_fam.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
PANTHERiPTHR12607. PTHR12607. 5 hits.
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform b1 Publication (identifier: Q21227-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSSSSDENE TTIHRTGSNT GGSGIYSQPR AGSSKRTSNV RHDVSDVDDE
60 70 80 90 100
EEHYARFRED TAIEVDDAIT VLLSSLHFEH KRDIVPTDED DNKLRELHEK
110 120 130 140 150
IFALITSESD VNRKRRLKKA LPASNCVREQ VYYLRRKPST PPASYYHRLN
160 170 180 190 200
AALHTIVKES FGEEYRKVAT VLGLVEALAE VLILEVHTFG INETNPGEHR
210 220 230 240 250
NIRKLIANAL TNLTYGQIHS KRRLCSYDGF IRCVVRIVIE SPNITQVYAG
260 270 280 290 300
LIRNLSWNAD SGMSEALQPT VHALSIAAVH AHTHRFDVTA TLSALWNLAG
310 320 330 340 350
HSVENKRTIC DTPNCLKVLA SLLSPDARFT SLVDSATGIL KYVSQYLANT
360 370 380 390 400
STHLELRSLL ITRMLTLLKS ASFTCVTNTL GAIANLIVKD PHMQQMIRQD
410 420 430 440 450
MAAVQQLNVL RNSNRDDIRT AVKSVLNTLN QPCSHRYGDM SHSVGGGATG
460 470 480 490 500
MQMLSEPQLQ MQTSHHAYHG TASPRLLSLR ATRASPGKYI QPQAQQQLIQ
510 520 530 540 550
TPQVDQRSSS LPRHFAVQRN GFVMAQSYNQ QMDQHQQQQM IYQLQQQQQI
560 570 580 590 600
MFQTEDQAQM EHHQQIMYLQ QQQQQFHQIQ QQQQMQKAQE ADPVPPTDDD
610 620 630 640 650
LDIPTSTVMG TRSNSERSLG SMNPGSVMTN WNSSLDTAAN SSRALSPVSY
660 670 680 690 700
NDIPASPTMC AQVFNLPKST ESEHHQLTSQ QQNTTHYSSG SANTMTRSDG
710 720 730 740 750
ATTVPMDNII TPTYAILNPI LVHEQTPNGT VPRKTSEELD SPDDVLPGPS
760 770 780 790 800
LEEEEGDYAI IGGAAQKTDD ELLTRSIQSE MPTSSSTPKM KVSPRLNGFF
810 820 830 840 850
SPTQKTTSSP AWSHPDTSPI PKSSSHRTQP NRRQDASDAD RLLMESIMSE
860 870 880 890 900
MPKSRIISPR LAGTQQYLEP EPERRSHSKN EEADRRDAFT ASHEPSDHNG
910 920 930 940 950
IDVARGSDWS PQQQLHRMES LESQASSEDS FGLTAEEPNS STSGAAANTM
960 970 980 990 1000
RFDDEIDASL PMDCVDDDDY DYTYDHFEDY EDEEDPDATQ FDDGVDAQLT
1010 1020 1030 1040 1050
IDCSMISSGS GSSQRNETTT TSRDSKALAT STPKGSASSL PGVRQATRVS
1060 1070 1080 1090 1100
TNGKSRLPVP KTNGSLVDKN PKPIIASRRP RLPPKPTLLK DKHYPEEDSI
1110 1120 1130 1140 1150
ENQTRDDTIY VNAPVVEAEQ ERIYMNALKQ QKNIEQSPSI GNGSPIAKSA
1160 1170 1180
IVTPYNYQKP PFTGRNNGEM SNEKSVTPNP KQMLVTIV
Note: No experimental confirmation available.Curated
Length:1,188
Mass (Da):131,990
Last modified:September 2, 2008 - v2
Checksum:iF3CDA53B5D3527A0
GO
Isoform a1 Publication (identifier: Q21227-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     552-553: Missing.

Show »
Length:1,186
Mass (Da):131,714
Checksum:iCBE672D78E2B245D
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_052859552 – 553Missing in isoform a. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013950 mRNA. Translation: AAC47747.1.
Z75712 Genomic DNA. Translation: CAB00045.1.
Z75712 Genomic DNA. Translation: CAB00048.1.
PIRiT23327.
T23330.
RefSeqiNP_001021547.1. NM_001026376.4. [Q21227-1]
NP_492217.2. NM_059816.4. [Q21227-2]
UniGeneiCel.16875.

Genome annotation databases

EnsemblMetazoaiK04G2.8b; K04G2.8b; WBGene00000156. [Q21227-1]
GeneIDi172591.
KEGGicel:CELE_K04G2.8.
UCSCiK04G2.8b. c. elegans. [Q21227-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAPR1_CAEEL
AccessioniPrimary (citable) accession number: Q21227
Secondary accession number(s): O62302
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: September 2, 2008
Last modified: November 22, 2017
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families