Q211D8 (RPOB_RHOPB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta
Short name=RNAP subunit beta
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta

Gene names

Name:	rpoB
Ordered Locus Names:	RPC_3458

Organism

Rhodopseudomonas palustris (strain BisB18) [Complete proteome] [HAMAP]

Taxonomic identifier

316056 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	1373 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01321
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01321
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1373	1373	DNA-directed RNA polymerase subunit beta HAMAP MF_01321		PRO_0000300388

Sequences

	Sequence		Length	Mass (Da)
	Q211D8 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: 5B56D2B1169AF3FB	FASTA	1,373	153,687
	10 20 30 40 50 60 MAQQTFTGRK RVRKFFGHIR EVAEMPNLIE VQKASYDQFL MVDEPVGGRL DEGLQAVFRS 70 80 90 100 110 120 VFPISDFSNT SMLEFVRYEF EPPKYDVDEC RQRGMTYAAP LKVTLRLIVF DIDEETGARS 130 140 150 160 170 180 VKDIKEQDVY MGDIPLMTMN GTFVVNGTER VIVSQMHRSP GVFFDHDKGK THSSGKLLFA 190 200 210 220 230 240 ARVIPYRGSW LDIEFDAKDI VFARIDRRRK IPVTSLMFAL GLDGEEILST FYKKIQYKKI 250 260 270 280 290 300 KEGWRVPFDS NRFRGYSTIN DLIDADTGKV VLEAGKKLTV RAARQLQEKG LKALRLSDEE 310 320 330 340 350 360 LVGNYLAEDL VNPKTGEIYA EAGEEITEKS LKGLNEQGYK ELPLLDIDHV NIGAYIRNTL 370 380 390 400 410 420 AADKNMTRED ALFDIYRVMR PGEPPTIDSA QNMFQSLFFD SERYDLSAVG RVKMNMRLEL 430 440 450 460 470 480 DAPDTHRTLR KEDILAVIKT LVGLRDGRGE IDDIDHLGNR RVRSVGELME NQYRIGLLRM 490 500 510 520 530 540 ERAIKERMSS VDIDTVMPQD LINAKPAAAA VREFFGSSQL SQFMDQTNPL SEITHKRRLS 550 560 570 580 590 600 ALGPGGLTRE RAGFEVRDVH PTHYGRICPI ETPEGPNIGL INSLATFARV NKYGFVETPY 610 620 630 640 650 660 RRCKDGRVTD EVVYLSAMEE GRYSVAQANV QLDPKGRFVE DLIVCRDGGT RDVVLIPADK 670 680 690 700 710 720 VDFMDVSPKQ LVSVAAALIP FLENDDANRA LMGSNMQRQA VPLVRAEAPF VGTGMEGVVA 730 740 750 760 770 780 RDSGAAIAAR RTGVIDQIDA TRIVIRATDD LDPTKSGVDI YRLMKYQRSN QSTCINQRPL 790 800 810 820 830 840 VKVGDMVAKG DIIADGPSTD LGELALGRNV LVAFMPWNGY NFEDSILLSE RIVKDDVFTS 850 860 870 880 890 900 IHIEEFEVMA RDTKLGPEEI TRDIPNVSEE ALKNLDEAGI VYIGAEVRAG DILVGKITPK 910 920 930 940 950 960 GESPMTPEEK LLRAIFGEKA SDVRDTSLRV PPGVQGTIVE VRVFNRHGVD KDERALAIER 970 980 990 1000 1010 1020 EEIERLAKDR DDEQAILDRN VYGRLADLLE NRQGIAGPKG FKKDTKITRA VIEEYPKSQW 1030 1040 1050 1060 1070 1080 WLFASPNDKL MAEIEAMRKQ YDESKKGLEQ RFLDKVEKLQ RGDELPPGVM KMVKVFVAVK 1090 1100 1110 1120 1130 1140 RKIQPGDKMA GRHGNKGVVS KIVPIEDMPF LEDGTHADIV LNPLGVPSRM NVGQILETHL 1150 1160 1170 1180 1190 1200 GWACAGLGKR IGQTIDAYYQ KQDLKPMRET LRKIYGEDET IKSLNDAELL ELARNLKPGV 1210 1220 1230 1240 1250 1260 PIATPVFDGA KEADIEEMLK LAGLDASGQS TVYDGRTGDT FDRKVTVGYI YMLKLHHLVD 1270 1280 1290 1300 1310 1320 DKIHARSIGP YSLVTQQPLG GKAQFGGQRF GEMEVWALEA YGAAYTLQEM LTVKSDDVAG 1330 1340 1350 1360 1370 RTKVYEAIVR GDDTFEAGIP ESFNVLVKEM RSLGLNVDLH NSKLATPTSE AAE

References

[1]

"Complete sequence of Rhodopseudomonas palustris BisB18." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Pelletier D.A., Kyrpides N., Anderson I., Oda Y., Harwood C.S., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BisB18.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000301 Genomic DNA. Translation: ABD88998.1.
RefSeq	YP_533317.1. NC_007925.1.
3D structure databases
HSSP	HSSP built from PDB template 2GHO based on UniProtKB Q9KWU7.
ProteinModelPortal	Q211D8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q211D8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3972126.
GenomeReviews	Gene locus RPC_3458 in contig CP000301_GR.
KEGG	rpc:RPC_3458.
PATRIC	23271742. VBIRhoPal29154_3553.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0085.
HOGENOM	HBG285547.
OMA	QRDLTYA.
PhylomeDB	Q211D8.
ProtClustDB	PRK00405.
Enzyme and pathway databases
BioCyc	RPAL316056:RPC_3458-MONOMER.
Family and domain databases
HAMAP	MF_01321. RNApol_bact_RpoB. [Tree]
InterPro	IPR010243. DNA-dir_RNA_pol_bsu_bac-type. IPR019462. DNA-dir_RNA_pol_bsu_external_1. IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
Gene3D	G3DSA:2.30.150.10. G3DSA:2.30.150.10. 1 hit. G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits. G3DSA:2.40.50.150. Ribosomal_L2. 2 hits.
KO	K03043.
PANTHER	PTHR20856:SF3. PTHR20856:SF3. 1 hit. PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 2 hits. PF04565. RNA_pol_Rpb2_3. 1 hit. PF10385. RNA_pol_Rpb2_45. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR02013. RpoB. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB_RHOPB

Accession

Primary (citable) accession number: Q211D8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	April 18, 2006
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program