Reviewed, UniProtKB/Swiss-Prot Q21181 (NAS19_CAEEL)

Names and origin

Protein names

Recommended name:
    Zinc metalloproteinase nas-19
    EC=3.4.24.21
Alternative name(s):
    Nematode astacin 19

Gene names

Name:	nas-19
ORF Names:	K03B8.5

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probable metalloprotease By similarity.
Catalytic activity	Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the peptidase M12A family. Contains 1 EGF-like domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	EGF-like domain Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 479	462	Zinc metalloproteinase nas-19		PRO_0000028923
Regions
	Domain	308 – 347	40	EGF-like
Sites
	Active site	222	1	By similarity
	Metal binding	221	1	Zinc; catalytic By similarity
	Metal binding	225	1	Zinc; catalytic By similarity
	Metal binding	231	1	Zinc; catalytic By similarity
Amino acid modifications
	Glycosylation	162	1	N-linked (GlcNAc...) Potential
	Glycosylation	393	1	N-linked (GlcNAc...) Potential
	Disulfide bond	313 ↔ 321		By similarity
	Disulfide bond	315 ↔ 335		By similarity
	Disulfide bond	337 ↔ 346		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q21181-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C1AE11ECC6951366	FASTA	479	54,849
	10 20 30 40 50 60 MYLKIFRLLL EIKSSILKIC VYFSSKNYGI LSISFAFLLI YFDVLYEKFP TDLHIPLDFS 70 80 90 100 110 120 YKNKVENGIN EHIAGGNIIQ HLEMVRLIHL IGAIILLFSY AYCGLSRLNE HDIEESYSHK 130 140 150 160 170 180 RVKRQFERLG TKWSYGVVNY YYADKNNEIK EMVESAIAYI ANHTCIRFNE DQNAVQRVQI 190 200 210 220 230 240 RMQQNWLCQS TVGAPGMSMS KPIGELSMLV QSCDTIGSIV HEFSHSLGRF HEHTRPDRDN 250 260 270 280 290 300 FMKVTTTVHE ARPRPSGMTT MYGPFEHGSV MMYHADTYGP GTMDPLDMDY KQTMGNRRVT 310 320 330 340 350 360 FYDMYKINQY YGCWCSKQLE CKNGGYTSPS DCSRCNCPKG FFGNLCDERQ QDSYELMAVN 370 380 390 400 410 420 NLWQSITIPF AYKPEPGSDG FYSTFVYITG KANSTIEITL EGLQDVICTA GCTVNGVEIK 430 440 450 460 470 FKEDSKITSP VVCCTDKPPY KNVFKSSHNP TIIELYSRTT LPSAVTFKYR FTNDKVVLG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"The astacin protein family in Caenorhabditis elegans." Moehrlen F., Hutter H., Zwilling R. Eur. J. Biochem. 270:4909-4920(2003) [PubMed: 14653817] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 186-218, NOMENCLATURE. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z74039 Genomic DNA. Translation: CAA98504.1. AJ561209 mRNA. Translation: CAD99211.1.
PIR	T23266.
RefSeq	NP_505893.1.
UniGene	Cel.34468
3D structure databases
HSSP	HSSP built from PDB template 1EGF based on UniProtKB P01132.
ModBase	Search...
Genome annotation databases
Ensembl	K03B8.5. Caenorhabditis elegans. [Contig view]
GeneID	186926.
KEGG	cel:K03B8.5.
NMPDR	fig|6239.3.peg.19719.
Organism-specific databases
WormBase	WBGene00003538. nas-19.
WormPep	K03B8.5. CE06079. [WorfDB]
Enzyme and pathway databases
BRENDA	3.4.24.21. 672.
Gene expression databases
ArrayExpress	Q21181.
Family and domain databases
InterPro	IPR013032. EGF-like_reg_CS. IPR006025. Pept_M_Zn_BS. IPR006026. Peptidase_M. IPR001506. Peptidase_M12A. IPR017050. Peptidase_M12A_nem. [Graphical view]
Pfam	PF01400. Astacin. 1 hit. [Graphical view]
PIRSF	PIRSF036365. Astacin_nematoda. 1 hit.
PRINTS	PR00480. ASTACIN.
SMART	SM00235. ZnMc. 1 hit. [Graphical view]
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. False negative. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	933484.

Entry information

Entry name

NAS19_CAEEL

Accession

Primary (citable) accession number: Q21181
Secondary accession number(s): Q7Z0N0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project