Reviewed, UniProtKB/Swiss-Prot Q21180 (NAS16_CAEEL)

Names and origin

Protein names

Recommended name:
    Zinc metalloproteinase nas-16
    EC=3.4.24.21
Alternative name(s):
    Nematode astacin 16

Gene names

Name:	nas-16
ORF Names:	K03B8.1

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	451 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probable metalloprotease By similarity.
Catalytic activity	Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the peptidase M12A family. Contains 1 EGF-like domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	EGF-like domain Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – ?		Potential
	Chain	? – 451		Zinc metalloproteinase nas-16		PRO_0000028920
Regions
	Domain	267 – 306	40	EGF-like
Sites
	Active site	176	1	By similarity
	Metal binding	175	1	Zinc; catalytic By similarity
	Metal binding	179	1	Zinc; catalytic By similarity
	Metal binding	185	1	Zinc; catalytic By similarity
Amino acid modifications
	Glycosylation	133	1	N-linked (GlcNAc...) Potential
	Glycosylation	363	1	N-linked (GlcNAc...) Potential
	Glycosylation	438	1	N-linked (GlcNAc...) Potential
	Disulfide bond	272 ↔ 280		By similarity
	Disulfide bond	274 ↔ 291		By similarity
	Disulfide bond	296 ↔ 305		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q21180-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 3F04B96F5606A5A6	FASTA	451	51,508
	10 20 30 40 50 60 MGNLFKFSLN CIKYRSENLE KSKMFYQYFY FHLTLSIVLI YGSDIGKDAN EHMSSVPKKI 70 80 90 100 110 120 IIEGTRQKRQ VVTKLFSPQW SNAKVFYCNA DSFSKLQLKK LKLKKNSDTV KRKLMKFAMN 130 140 150 160 170 180 FISSQTCVTF EENCTISTRI KFVDSTFCAS YVGMINSVQE IYFPDWCMRF GSAVHELMHA 190 200 210 220 230 240 LGVLHTHARF DRDNFLNVNL NKDDEDDSNF EIVSPPFSIN VVPYEYGSTL HYTADVSGTN 250 260 270 280 290 300 SLLPKQMEYY RTLGNRRVTF YDMLTINTAY NCKCPSELLC ANGGYTNPSN CLECICPLGY 310 320 330 340 350 360 GGVLCDRVVA CSVQLSADSY WKGSWISVGS SVLRDTTDPV KAFISINAPK DKIIEVKIVK 370 380 390 400 410 420 IENFSCDSGC NNNGVEIKYM GDPRITNPII CCENQVDPSN KGYKAKLNPL LINIYTFLGK 430 440 450 NKVTFHYRYV NERLSSYNKT TNGYDNYEYY A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"The astacin protein family in Caenorhabditis elegans." Moehrlen F., Hutter H., Zwilling R. Eur. J. Biochem. 270:4909-4920(2003) [PubMed: 14653817] [Abstract] Cited for: IDENTIFICATION, NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z74039 Genomic DNA. Translation: CAA98503.1.
PIR	T23265.
RefSeq	NP_505889.1.
UniGene	Cel.32979
3D structure databases
HSSP	HSSP built from PDB template 1IAE based on UniProtKB P07584.
ModBase	Search...
Protein family/group databases
MEROPS	M12.310.
Genome annotation databases
Ensembl	K03B8.1. Caenorhabditis elegans. [Contig view]
GeneID	186922.
KEGG	cel:K03B8.1.
NMPDR	fig|6239.3.peg.19715.
Organism-specific databases
WormBase	WBGene00003535. nas-16.
WormPep	K03B8.1. CE06075. [WorfDB]
Phylogenomic databases
OMA	Q21180. CTISTRI.
Enzyme and pathway databases
BRENDA	3.4.24.21. 672.
Gene expression databases
ArrayExpress	Q21180.
Family and domain databases
InterPro	IPR013032. EGF-like_reg_CS. IPR006025. Pept_M_Zn_BS. IPR006026. Peptidase_M. IPR001506. Peptidase_M12A. IPR017050. Peptidase_M12A_nem. [Graphical view]
Pfam	PF01400. Astacin. 1 hit. [Graphical view]
PIRSF	PIRSF036365. Astacin_nematoda. 1 hit.
PRINTS	PR00480. ASTACIN.
SMART	SM00235. ZnMc. 1 hit. [Graphical view]
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. False negative. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	933468.

Entry information

Entry name

NAS16_CAEEL

Accession

Primary (citable) accession number: Q21180

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project