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Protein

Zinc metalloproteinase nas-16

Gene

nas-16

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Probable metalloprotease.By similarity

Catalytic activityi

Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi175 – 1751Zinc; catalyticPROSITE-ProRule annotation
Active sitei176 – 1761PROSITE-ProRule annotation
Metal bindingi179 – 1791Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi185 – 1851Zinc; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM12.A30.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc metalloproteinase nas-16 (EC:3.4.24.21)
Alternative name(s):
Nematode astacin 16
Gene namesi
Name:nas-16
ORF Names:K03B8.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiK03B8.1; CE06075; WBGene00003535; nas-16.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 451Zinc metalloproteinase nas-16PRO_0000028920
Signal peptidei1 – ?Sequence analysis

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi133 – 1331N-linked (GlcNAc...)Sequence analysis
Disulfide bondi272 ↔ 280By similarity
Disulfide bondi274 ↔ 291By similarity
Disulfide bondi296 ↔ 305By similarity
Glycosylationi363 – 3631N-linked (GlcNAc...)Sequence analysis
Glycosylationi438 – 4381N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ21180.

Interactioni

Protein-protein interaction databases

STRINGi6239.K03B8.1.

Structurei

3D structure databases

ProteinModelPortaliQ21180.
SMRiQ21180. Positions 111-276.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini267 – 30640EGF-likeAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase M12A family.Curated
Contains 1 EGF-like domain.Curated

Keywords - Domaini

EGF-like domain, Signal

Phylogenomic databases

eggNOGiKOG3714. Eukaryota.
ENOG410ZPX7. LUCA.
GeneTreeiENSGT00760000119227.
HOGENOMiHOG000022534.
InParanoidiQ21180.
KOiK08076.
OMAiEENCTIS.
PhylomeDBiQ21180.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR017050. Metallopeptidase_nem.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
[Graphical view]
PIRSFiPIRSF036365. Astacin_nematoda. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00235. ZnMc. 1 hit.
[Graphical view]
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q21180-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNLFKFSLN CIKYRSENLE KSKMFYQYFY FHLTLSIVLI YGSDIGKDAN
60 70 80 90 100
EHMSSVPKKI IIEGTRQKRQ VVTKLFSPQW SNAKVFYCNA DSFSKLQLKK
110 120 130 140 150
LKLKKNSDTV KRKLMKFAMN FISSQTCVTF EENCTISTRI KFVDSTFCAS
160 170 180 190 200
YVGMINSVQE IYFPDWCMRF GSAVHELMHA LGVLHTHARF DRDNFLNVNL
210 220 230 240 250
NKDDEDDSNF EIVSPPFSIN VVPYEYGSTL HYTADVSGTN SLLPKQMEYY
260 270 280 290 300
RTLGNRRVTF YDMLTINTAY NCKCPSELLC ANGGYTNPSN CLECICPLGY
310 320 330 340 350
GGVLCDRVVA CSVQLSADSY WKGSWISVGS SVLRDTTDPV KAFISINAPK
360 370 380 390 400
DKIIEVKIVK IENFSCDSGC NNNGVEIKYM GDPRITNPII CCENQVDPSN
410 420 430 440 450
KGYKAKLNPL LINIYTFLGK NKVTFHYRYV NERLSSYNKT TNGYDNYEYY

A
Length:451
Mass (Da):51,508
Last modified:November 1, 1996 - v1
Checksum:i3F04B96F5606A5A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74039 Genomic DNA. Translation: CAA98503.1.
PIRiT23265.
RefSeqiNP_505889.1. NM_073488.1.
UniGeneiCel.32979.

Genome annotation databases

EnsemblMetazoaiK03B8.1; K03B8.1; WBGene00003535.
GeneIDi186922.
KEGGicel:CELE_K03B8.1.
UCSCiK03B8.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74039 Genomic DNA. Translation: CAA98503.1.
PIRiT23265.
RefSeqiNP_505889.1. NM_073488.1.
UniGeneiCel.32979.

3D structure databases

ProteinModelPortaliQ21180.
SMRiQ21180. Positions 111-276.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.K03B8.1.

Protein family/group databases

MEROPSiM12.A30.

Proteomic databases

PaxDbiQ21180.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiK03B8.1; K03B8.1; WBGene00003535.
GeneIDi186922.
KEGGicel:CELE_K03B8.1.
UCSCiK03B8.1. c. elegans.

Organism-specific databases

CTDi186922.
WormBaseiK03B8.1; CE06075; WBGene00003535; nas-16.

Phylogenomic databases

eggNOGiKOG3714. Eukaryota.
ENOG410ZPX7. LUCA.
GeneTreeiENSGT00760000119227.
HOGENOMiHOG000022534.
InParanoidiQ21180.
KOiK08076.
OMAiEENCTIS.
PhylomeDBiQ21180.

Miscellaneous databases

NextBioi933468.
PROiQ21180.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR017050. Metallopeptidase_nem.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
[Graphical view]
PIRSFiPIRSF036365. Astacin_nematoda. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00235. ZnMc. 1 hit.
[Graphical view]
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "The astacin protein family in Caenorhabditis elegans."
    Moehrlen F., Hutter H., Zwilling R.
    Eur. J. Biochem. 270:4909-4920(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, NOMENCLATURE.

Entry informationi

Entry nameiNAS16_CAEEL
AccessioniPrimary (citable) accession number: Q21180
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: November 1, 1996
Last modified: November 11, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.