Reviewed, UniProtKB/Swiss-Prot Q21179 (NAS18_CAEEL)

Names and origin

Protein names

Recommended name:
    Zinc metalloproteinase nas-18
    EC=3.4.24.21
Alternative name(s):
    Nematode astacin 18

Gene names

Name:	nas-18
ORF Names:	K03B8.3

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probable metalloprotease By similarity.
Catalytic activity	Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the peptidase M12A family. Contains 1 EGF-like domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	EGF-like domain Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – ?		Potential
	Chain	? – 410		Zinc metalloproteinase nas-18		PRO_0000028922
Regions
	Domain	256 – 295	40	EGF-like
Sites
	Active site	169	1	By similarity
	Metal binding	168	1	Zinc; catalytic By similarity
	Metal binding	172	1	Zinc; catalytic By similarity
	Metal binding	178	1	Zinc; catalytic By similarity
Amino acid modifications
	Glycosylation	37	1	N-linked (GlcNAc...) Potential
	Glycosylation	126	1	N-linked (GlcNAc...) Potential
	Glycosylation	157	1	N-linked (GlcNAc...) Potential
	Glycosylation	192	1	N-linked (GlcNAc...) Potential
	Glycosylation	195	1	N-linked (GlcNAc...) Potential
	Glycosylation	208	1	N-linked (GlcNAc...) Potential
	Glycosylation	227	1	N-linked (GlcNAc...) Potential
	Glycosylation	397	1	N-linked (GlcNAc...) Potential
	Disulfide bond	261 ↔ 269		By similarity
	Disulfide bond	263 ↔ 283		By similarity
	Disulfide bond	285 ↔ 294		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q21179-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 0101D9E1D151EA6C	FASTA	410	46,271
	10 20 30 40 50 60 MCASCLHNAN AASFLMETFS RHRLNFICIG KMNIFYNVTS LDNKYSFHNP SAPVPHSSHA 70 80 90 100 110 120 LSSRQAHFTS FRAPVAPPSR PRRSQLPSSA KLLYCFDSSF STEKRELMLF SMNFISSQTC 130 140 150 160 170 180 VTFEENCSIP NRVQLVNGKG CSSYIGMNNI VQHLTFNDTC IDFGTAVHEL MHALGVIHTH 190 200 210 220 230 240 SRLDRDNFLN INLTNVSKEM MHNYAIFNQS TNVVPYEYGS TMHYYANIST MFPKKSEYSA 250 260 270 280 290 300 TLGIGRVTFY DMVILNTAYN CKCPNELLCG NGGYTNPSKC SECICPLGYG GVLCDRPSLI 310 320 330 340 350 360 GKDTGLPLEV KDKIIEVKVV GIDNFFSYPT CLINGLEIKY MGDPRITNPI YCSADLINGK 370 380 390 400 410 SFRSKLNPLL MNIHTLFGKN KVTFHYRYVT ERLSGYNKTT NGYDNYEYYD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"The astacin protein family in Caenorhabditis elegans." Moehrlen F., Hutter H., Zwilling R. Eur. J. Biochem. 270:4909-4920(2003) [PubMed: 14653817] [Abstract] Cited for: NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z74039 Genomic DNA. Translation: CAA98502.1.
PIR	T23264.
RefSeq	NP_505891.1.
UniGene	Cel.27176
3D structure databases
HSSP	HSSP built from PDB template 1IAE based on UniProtKB P07584.
ModBase	Search...
Protein family/group databases
MEROPS	M12.310.
Genome annotation databases
Ensembl	K03B8.3. Caenorhabditis elegans. [Contig view]
GeneID	186924.
KEGG	cel:K03B8.3.
NMPDR	fig|6239.3.peg.19717.
Organism-specific databases
WormBase	WBGene00003537. nas-18.
WormPep	K03B8.3. CE06077. [WorfDB]
Enzyme and pathway databases
BRENDA	3.4.24.21. 672.
Gene expression databases
ArrayExpress	Q21179.
Family and domain databases
InterPro	IPR013032. EGF-like_reg_CS. IPR006025. Pept_M_Zn_BS. IPR006026. Peptidase_M. IPR001506. Peptidase_M12A. IPR017050. Peptidase_M12A_nem. [Graphical view]
Pfam	PF01400. Astacin. 1 hit. [Graphical view]
PIRSF	PIRSF036365. Astacin_nematoda. 1 hit.
PRINTS	PR00480. ASTACIN.
SMART	SM00235. ZnMc. 1 hit. [Graphical view]
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. False negative. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	933476.

Entry information

Entry name

NAS18_CAEEL

Accession

Primary (citable) accession number: Q21179

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project